• Animal cells and systems
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• v.11 no.2
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• pp.129-134
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• 2007

The initiation of eukaryotic DNA replication requires assembly of the pre-replicative complex (Pre-RC) through the concerted action of Orc, Cdc6, Cdt1 and Mcm2-7 complex during G1 phase. The pre-RC assembly licenses individual replication origins for the initiation of DNA replication and sufficient number of the pre-RC is essential for proper progression of S phase. However, it is not well known how cells recognize the completion of the pre-RC assembly before G1-S transition. In order to understand the cellular responses to the defects in pre-RC assembly, we depleted the known components of pre-RC proteins using the small interference RNAs in HeLa cells. Although the defects of pre-RC assembly by the depletion of the pre-RC proteins such as Orc2, Cdt1, Mcm2 & Mcm10 did not elicit the activation of Chk1- or Chk2-dependent checkpoint pathways, these cells still showed significant decrease in the cellular level of Cdc25A proteins. These results suggests that a novel checkpoint pathway exist in HeLa cells, which is not dependent upon Chk1 or Chk2 proteins and play essential roles in the cellular responses to the defects in the pre-RC assembly. Also, among those four proteins tested in this study, the depletion of Mcm10 and Cdt1 proteins significantly increased the apoptotic cell death in HeLa cells, suggesting that these proteins not only play roles in the pre-RC assembly, but also are involved in the checkpoint responses to the defects in the pre-RC assembly.

• Journal of the Korea Concrete Institute
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• v.16 no.1 s.79
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• pp.88-93
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• 2004

The objective of this study was to investigate applicability of epoxy injection method to cracked RC beams and structural behavior of repaired RC beams considering pre-loading conditions. For this purpose, five test beams were fabricated under two experimental variables. The main variables of this experimental study were pre-loading conditions and repair methods. The two pre-loading conditions were selected as $70\%$ and $90\%$ of nominal strength and the repair methods were to repair the cracked RC beams under free loading after crack and sustained loading. The comparative study was executed to evaluate effects of pre-loading conditions on the structural behavior of the cracked RC beams after crack-repair. The strains of reinforcement and concrete and deflections of beams at each loading step were measured and evaluated. As the results of this study, repair methods have much influence on structural behavior of epoxy injected RC beams and epoxy injection method for cracks of RC structures is appeared to be efficient.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.35 no.1
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• pp.45-55
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• 2022

Because boundary confinement details proposed in the current design standards are significantly inferior in workability and production quality, it is necessary to develop boundary confinement details of RC structural walls that are capable of ensuring seismic performance and workability. With the recent development of the wire rod manufacturing technology, various pre-fabricated continuous hoop details can be manufactured. In this study, an analysis was conducted on the moment-curvature relationship of RC structural walls to which the pre-fabricated continuous hoop details were applied. According to the nonlinear cross-section analysis, the RC structure wall to which the details of the pre-fabricated continuous hoop details are applied can ensure seismic performance as the area of the pre-fabricated continuous hoop increases. Based on these research results, when applying the pre-fabricated continuous hoop in detail, it is necessary to secure the area of the pre-fabricated continuous hoop as much as the area of the existing boundary confinement.

• Journal of the Architectural Institute of Korea Structure & Construction
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• v.34 no.11
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• pp.19-26
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• 2018

This paper presents the shear strengthening effect of externally post-tensioning (EPT) method using high-strength steel rod in pre-cracked reinforced concrete (RC) beams. Three- and four-point bending tests were performed on a total of 8 specimens by adjusting the strengthening depths in the deviator position of EPT. The effective strengthening depths were 435, 535, and 610 mm. The pre-loading up to about 2/3 of ultimate load capacity measured in unstrengthened RC beam were applied in the beam to be post-tensioned. The EPT method was then applied to the pre-damaged RC beams and re-loading was added until the end of the test. EPT restored deflections of 3 mm or more, which account for about 40% of deflection when the pre-loading was applied. The shear strengthening increases more than 3 times and 36~107% in terms of the stiffness and load-carrying capacity compared to unstrengthening RC beams. The increased load-carrying capacities of the post-tensioned beam with strengthening depths of 435 and 535 mm are almost the same as 36~61%, and those of 610 mm are 84~107%, which shows the greatest shear strengthening effect.

• Journal of the Institute of Electronics Engineers of Korea CI
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• v.42 no.6
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• pp.29-36
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• 2005

RFID(Radio Frequency Identification) will be used for recognizing things and offering distinctive information in Ubiquitous environment. But we are not ready to provide security service to RFID. Therefore we propose the approach of safe RFID system which provides the solution for eavesdrop, forgery, and modification based on Pre-Distribution protocol and RC5 security algorithm. First We distributes the secret key to the Tag with the Reader that is major subject on RFID system using Pre-Distribution protocol. Then we will provide to various security services and privacy protection using RC5 security algorithm. The proposed safe RFID system simulated on ATmega128 evaluate environment.

• Steel and Composite Structures
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• v.17 no.4
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• pp.371-386
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• 2014

This paper presents a tri-linear restoring force model based on the test results of 12 circular RC columns strengthened by CFRP strips under low cyclic loading. The pre-stress of CFRP strips and axial load ratio of specimens are considered as the affect parameters of the proposed model. All essential characteristics of the hysteretic behavior of the proposed model, including the hysteretic rules, main performance points, strength degradation, stiffness degradation and confinement effects are explicitly analyzed. The calculated results from the proposed model are in good agreement with the experimental results, which shows that the recommended model can be reliably used for seismic behavior predictions of circular RC columns strengthened by pre-stressed CFRP strips.

• Journal of the Korean Society of Safety
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• v.31 no.5
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• pp.102-108
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• 2016

This paper presents a new strength method of underground box structures under additional surface load. An L-bracing using pre-flexed steel member threads called the "Pre-flex strength method" is used to improve capacity of the RC box structure under earth pressure due to additional surface load. The pre-flexed steel member is fixed the top and bottom of the structure after chemical anchor was installed by drilling hole on the box structure. The structural performance was evaluated analytically. 3 types of underground RC box structure were used; $2.0m{\times}2.0m$, $3.0m{\times}3.0m$ and $4.0m{\times}4.0m$. For the performance evaluation, structure analysis were performed on moment and shear resisting structures with and without pre-flex strength method. Numerical results confirmed that the proposed strength member system installed on underground RC box structures enhanced the strength capacity. The feasible region of the proposed pre-flex strength method in accordance with the earth pressure due to additional surface depth was evaluated.

• Structural Monitoring and Maintenance
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• v.5 no.2
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• pp.189-204
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• 2018

This paper examines the seismic responses of a reinforced concrete (RC) frame core-tube building with pre-pressed spring self-centering energy dissipation (PS-SCED) braces. The PS-SCED brace system consists of friction devices for energy dissipation, pre-pressed combination disc springs for self-centering and tube members as guiding elements. A constitutive model of self-centering flag-shaped hysteresis for PS-SCED brace is developed to better simulate the seismic responses of the RC frame core-tube building with PS-SCED braces, which is also verified by the tests of two braces under low cyclic reversed loading. Results indicate that the self-centering and energy dissipation capabilities are well predicted by the proposed constitutive model of the PS-SCED brace. The structure with PS-SCED braces presents similar peak story drift ratio, smaller peak acceleration, smaller base shear force and much smaller residual deformations as compared to the RC frame core-tube building with bucking-restrained braces (BRBs).

• International Journal of Concrete Structures and Materials
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• v.11 no.1
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• pp.171-183
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• 2017

Strengthening reinforced concrete (RC) beams with openings by using aramid fiber reinforcement polymers (AFRP) on the beams' surfaces offers a useful solution for upgrading concrete structures to carry heavy loads. This paper presents a repairing technique of the AFRP sheets that effectively strengthens RC beams, controls both the failure modes and the stress distribution around the beam chords and enhances the serviceability (deflection produced under working loads be sufficiently small and cracking be controlled) of pre-cracked RC beams with openings. To investigate the possible damage that was caused by the service load and to simulate the structure behavior in the site, a comprehensive experimental study was performed. Two unstrengthened control beams, four beams that were pre-cracked before the application of the AFRP sheets and one beam that was strengthened without pre-cracking were tested. Cracking was first induced, followed by repair using various orientations of AFRP sheets, and then the beams were tested to failure. This load was kept constant during the strengthening process. The results show that both the preexisting damage level and the FRP orientation have a significant effect on strengthening effectiveness and failure mode. All of the strengthened specimens exhibited higher capacities with capacity enhancements ranging from 21.8 to 66.4%, and the crack width reduced by 25.6-82.7% at failure load compared to the control beam. Finally, the authors present a comparison between the experimental results and the predictions using the ACI 440.2R-08 guidelines.

• Journal of the Korea institute for structural maintenance and inspection
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• v.10 no.5
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• pp.121-129
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• 2006

This study look into the mechanisms of growth and magnification of the cracks and delamination in the pre-cracked RC beams repaired with carbon fiber sheets. The experimental parameters were loading type, loading speed and crack. In the experiments, it was confirmed that a failure of beams began with development and propagation of the stepped delamination in the below the loading point due to the rapid change of shear force, but mechanisms of the failure were not influenced with loading type, loading speed and pre-cracks. Particularly, in the case of beams having the pre-cracks, growth of crack concentrated at the special crack below the loading point and led to failure of the beam by delamination due to magnification of crack.