• Reproductive and Developmental Biology
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• 제30권4호
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• pp.279-285
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• 2006

Vitrification has been suggested to be an effective method for the cryopreservation of human ES cells. However, the efficiency of vitrification with different vehicles remains a matter of ongoing controversy. The objective of this study was to assess the efficiency of cryopreservation in human ES cells by vitrification using different vehicles. A human ES cell line and a variety of vehicles, including micro-droplet (MD), open-pulled straw (OPS) and electron microscopic grid (EM-grid), were employed in an attempt to assess vitrification efficiency. In order to evaluate the survivability and the undifferentiated state of the post-vitrified human ES cells, we conducted alkaline phosphatase staining and characterization via both RT-PCR and immunofluorescence assays. The survival rates of the post-vitrified human ES cells using MD, OPS and EM-grid were determined to be 61.5%, 66.6% and 53.8%, respectively. There also exist significant differences between slow-freezing and vitrification (p<0.01). However, no significant differences were detected between the vehicle types. Finally, the pluripotency of human ES cells after thawing was verified by teratoma formation. Cryopreservation using vitrification is more effective than slow-freezing, and the efficiency of vehicles proved effective with regard to the preservation of human ES cells.

• Acta Via Serica
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• 제5권1호
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• pp.1-22
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• 2020

The paper discusses how the new EU Strategy towards Central Asia issued in May 2019 might be analyzed through the lens of the intensely debated transformations from the liberal to a post-liberal international order. The author claims that the EU's normative power is transforming from the post-Cold War predominantly liberal/ value-based approach, with democracy and human rights at its core, to a set of more technical tools and principles of good governance and effective management of public administration. The paper problematizes a nexus between the dynamics of the EU's nascent post-liberalism and the geopolitical challenges of the EU's growing engagement with illiberal regimes, focuses on direct encounters between the post-liberal EU and the illiberal elites in Central Asia, and seeks to find out the impact of these connections upon the EU's international subjectivity. In this context geopolitical dimensions of EU foreign and security policies, along with the specificity of the EU's geopolitical actorship in Central Asia, are discussed.

• 한국동물학회:학술대회논문집
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• 한국동물학회 2001년도 한국생물과학협회 학술발표대회
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• pp.81-85
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• 2001

No Abstract, See Full Text

• 한국실내디자인학회논문집
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• 제41호
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• pp.246-255
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• 2003

All art forms are the results of the artist's response to the philosophical, sociological, and cultural tendencies of the period in which the artist lives. Post-modern period differentiates itself from Modern period in many aspects. One of these is the emphasis on phenomena which are ephemeral and changing. This paper looks at the perceptive and cognitive bases of these expressions of change in space design and their affordances on human behavior The first chapter provides the purpose and the background of the research. It studies the philosophical, sociological and cultural characteristics of the Post-modern era which bring forth expressions of time ＆ change in space design. The second chapter explains two basic approaches which can be applied to the perception and cognition of the environment. The third chapter deals with the perceptional and cognitive bases, and the human affordances of the expressions of change in space design. Finally this paper will analyze the types of space design which show this tendency : change by the technology, change by the participation of viewers, and change by the natural and chance. In each type, there will be analyses of examples. this will lead us to the understanding of how the space design including time & change expresses the spirit of the age and how it can play positive roles in human psychology.

• 복식
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• 제65권6호
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• pp.99-111
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• 2015

The purpose of this study is to identify the expression methods of uncanny images shown in contemporary fashion through the visual expression methods of plastic arts. The study results are as following. First, there is a concept-forming element of 'The return of Repressed', which is a psychological mechanism for generating uncanny sets to the component of 'repression' and 'return'. 'Repression' herein consisted of 'unconsciousness', 'the other', and 'anxiety', while the return was composed of 'trauma' and 'repetition compulsion'. Second, there are visual expression methods of the uncanny in contemporary art. The subject of 'repressed things' was expressed as grotesque and having a terrible look. 'Fragmented body' was analyzed as 'dismantling' by mutilation, 'combination' placing cut body parts in other places, and 'damage' suffering from an illness or accident. 'Fear of death' was shown as 'anatomy' reminiscent of death and 'iconography of death' meaning return of the dead. 'Post-human' was a surrealistic being such as a monster and mechanized human, and was analyzed as 'gene variation' and 'cyborg'. Third, the methods of visual expression of the uncanny reflected on contemporary fashion was analyzed as 'fragmented body', 'fear of death', and 'post-human'. 'Fragmented body' appeared as the object reminiscent of the fragmented body, 'dismantling and combination of the body', and 'damage of the body' through the distortion of the clothing construction principle. 'Fear of death' visualized the characteristic elements of the subject reminiscent of death as 'iconography of death' and 'symbolic object of death' through the motif. 'Post-human' was the description of the subject beyond the body's function and form, and was shown as 'gene variation' and 'cyborg'.

• Clinical and Experimental Reproductive Medicine
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• 제45권4호
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• pp.177-182
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• 2018

Objective: To investigate the effects of in vitro myo-inositol (Myo-Ins) supplementation of cryopreserved human semen on the cryo-survival rate (CSR). Methods: Semen samples were obtained from 41 infertile men. Following routine semen analysis, each sample was divided into two equal aliquots (0.5 mL each). One aliquot was treated with 1 mg of Myo-Ins dissolved in $10{\mu}L$ of sperm preparation medium. The second aliquot was treated with $10{\mu}L$ of the same medium (control). Both aliquots were incubated for 20 minutes prior to freezing to slow the freezing process. The frozen samples were examined for post-thaw percentages of total motility (TM), progressive motility (PM), and the CSR, defined as the percentage of post-thaw TM divided by the percentage of pre-freeze TM and multiplied in 100. The results were expressed as median and interquartile range (25th and 75th percentiles). Results: The pre-freeze TM (50% [30%-50%]) and PM (35% [20%-35%]) were significantly higher than the post-thaw TM and PM in the MyoIns group (15% [10%-35%] and 10% [5%-20%]; p<0.001 and p<0.001, respectively) and the control group (10% [6%-30%] and 5% [3%-15%]; p<0.001 and p<0.001, respectively). The CSR of the 41 semen aliquots supplemented with Myo-Ins (40% [25%-70%]) was significantly higher than that of the control samples (30% [13%-58%], p=0.041). The CSR of the 26 abnormal semen samples that were supplemented with Myo-Ins (38% [20%-50%]) was significantly higher than that of the control samples (23% [12%-30%], p=0.031). Conclusion: In vitro Myo-Ins supplementation of ejaculated human sperm from infertile men resulted in a significant increase in the CSR in samples with abnormal pre-freeze sperm parameters.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2005년도 제36회 하계학술대회 논문집 D
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• pp.2959-2961
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• 2005

This study presents a human sensibility evaluation algorithm based on the personality-group templates of EEGs. For this objective, 16-channel EEG signals of 10 adults are collected. After Preprocessing of EEG, various EEG Parameters are estimated and compared. The proposed algorithm uses LP coefficients, neural network and pre-/post-processing techniques. The results showed good performance in human sensibility evaluation.

• Biotechnology and Bioprocess Engineering:BBE
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• 제5권5호
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• pp.307-312
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• 2000

As the first assembly of the human genome was announced on June 26, 2000, we have entered post genome era. The genome sequence represents a new starting point for science and medicine with possible impact on research across the life sciences. In this review I tried to offer brief summaries of history and progress of the Human Genome Project and two major challenges ahead, functional genomics and DNA sequence variation research.

• 반도체디스플레이기술학회지
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• 제20권3호
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• pp.120-124
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• 2021

Recently, human attribute recognition (HAR) attracts a lot of attention due to its wide application in video surveillance systems. Recent deep-learning-based solutions for HAR require time-consuming training processes. In this paper, we propose a post-processing technique that utilizes the historical video frames to improve prediction results without invoking re-training or modifying existing deep-learning-based classifiers. Experiment results on a large-scale benchmark dataset show the effectiveness of our proposed method.

• 가족자원경영과 정책
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• 제8권1호
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• pp.61-78
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• 2004

The purpose of this study was to compare the consumers' satisfactions between those who prefer internet shopping and those who prefer CATV home shopping. This study examined both on-purchase satisfactions and post-purchase satisfactions. For on-purchase satisfactions, this study investigated informational satisfactions, convenience satisfactions, and price satisfactions, and for the post-purchase satisfactions, it examined overall satisfactions, delivery-related, and exchange or refund related satisfactions. The data(n=376) were obtained from a questionnaire. The data included 185 consumers of internet shopping, and 182 consumers of TV home shopping, and were analyzed by t-tests or chi-square test to compare those consumers' characteristics and satisfactions. The findings of this study are as follows: First, men aged 20s and those who had higher education, higher incomes were more likely to prefer internet shopping. Second, those who like TV home shopping were likely to search just one channel and to use less than 30 minutes to decide to purchase. Third, generally, those who prefer internet shopping showed higher scores of on-purchase satisfactions, and overall post-purchase satisfactions. However, TV home shopping consumers had higher scores of delivery-related satisfactions.