Clinical and Experimental Reproductive Medicine

  • 제45권4호
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  • Pages.177-182
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  • 2018
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  • 2233-8233(pISSN)
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  • 2233-8241(eISSN)
대한생식의학회 (The Korean Society for Reproductive Medicine)
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Increased cryo-survival rate in ejaculated human sperm from infertile men following pre-freeze in vitro myo-inositol supplementation

  • Saleh, Ramadan (Department of Dermatology, Venereology and Andrology, Faculty of Medicine, Sohag University) ;
  • Assaf, Hanan (Department of Dermatology, Venereology and Andrology, Faculty of Medicine, Sohag University) ;
  • Abd El Maged, Wafaa M. (Department of Dermatology, Venereology and Andrology, Faculty of Medicine, Sohag University) ;
  • Elsuity, Mohamed (Department of Dermatology, Venereology and Andrology, Faculty of Medicine, Sohag University) ;
  • Fawzy, Mohamed (Ajyal IVF Center, Ajyal Hospital)
  • 투고 : 2018.05.23
  • 심사 : 2018.10.23
  • 발행 : 2018.12.31
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초록

Objective: To investigate the effects of in vitro myo-inositol (Myo-Ins) supplementation of cryopreserved human semen on the cryo-survival rate (CSR). Methods: Semen samples were obtained from 41 infertile men. Following routine semen analysis, each sample was divided into two equal aliquots (0.5 mL each). One aliquot was treated with 1 mg of Myo-Ins dissolved in $10{\mu}L$ of sperm preparation medium. The second aliquot was treated with $10{\mu}L$ of the same medium (control). Both aliquots were incubated for 20 minutes prior to freezing to slow the freezing process. The frozen samples were examined for post-thaw percentages of total motility (TM), progressive motility (PM), and the CSR, defined as the percentage of post-thaw TM divided by the percentage of pre-freeze TM and multiplied in 100. The results were expressed as median and interquartile range (25th and 75th percentiles). Results: The pre-freeze TM (50% [30%-50%]) and PM (35% [20%-35%]) were significantly higher than the post-thaw TM and PM in the MyoIns group (15% [10%-35%] and 10% [5%-20%]; p<0.001 and p<0.001, respectively) and the control group (10% [6%-30%] and 5% [3%-15%]; p<0.001 and p<0.001, respectively). The CSR of the 41 semen aliquots supplemented with Myo-Ins (40% [25%-70%]) was significantly higher than that of the control samples (30% [13%-58%], p=0.041). The CSR of the 26 abnormal semen samples that were supplemented with Myo-Ins (38% [20%-50%]) was significantly higher than that of the control samples (23% [12%-30%], p=0.031). Conclusion: In vitro Myo-Ins supplementation of ejaculated human sperm from infertile men resulted in a significant increase in the CSR in samples with abnormal pre-freeze sperm parameters.

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참고문헌

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