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Ultrastructure of the Fertilized Egg Envelope in Cichlasoma managuensis, Cichlidae, Teleost (경골어류 시클리드과 Cichlasoma managuensis의 수정란 난막 미세구조)

  • Kim, Dong-Heui;Chang, Byung-Soo;Teng, Yung-Chien;Kim, Seok;Joo, Kyung-Bok;Lee, Kyu-Jae
    • Applied Microscopy
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    • v.39 no.1
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    • pp.9-15
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    • 2009
  • The ultrastructure of the fertilized egg envelope in Cichlasoma managuensis belonging to Cichlidae were investigated by routine light and electron microscopes. The fertilized eggs of Cichlasoma managuensis was of the light yellow, non-transparent, ellipsoidal, adhesive and nonfloted type. The size of fertilized egg was the major axis $1.92{\pm}0.08\;mm$, the minor axis $1.43{\pm}0.04\;mm$. The egg envelopes have a single micropyle, which is thought to the pathway of sperm in the area of the animal pole. An outer surface of fertilized egg envelope was covered by a adhesive reticular structures and the fertilized egg envelopes consisted of two distinct layers; an outer adhesive layer and an inner layer of $13{\sim}15$ horizontal lower electron density lamellae alternating with interlamellae of higher electron density. The external shape of fertilized egg is common trait of fishes belonging to Cichlidae and these ultrastructural characters of fertilized egg envelope can be utilized in taxonomy of teleost.

Comparative Ultranstructures of the Fertilized Egg Envelopes from Three-spot gourami, Pearl gourami and Marble gourami, Belontiidae, Teleost (경골어류 등목어과 Three-spot gourami, Pearl gourami 및 Marble gourami의 수정란 난막 미세구조 비교)

  • Kim, Dong-Heui;Deung, Young-Kun;Kim, Wan-Jong;Reu, Dong-Suck;Kang, Song Jian
    • Applied Microscopy
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    • v.29 no.3
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    • pp.343-351
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    • 1999
  • The structures of the fertilized egg envelope from three species, three-spot gourami (Trichogaster trichopterus), pearl gourami (Trichogaster leeri) and marble gourami (Trichogaster trichopterus trichopterus) belong to Belontiidae were observed, utilizing light, scanning and transmission electron microscopes. In all three species, the fertilized eggs were the colorless, transparent, spherical, adhesive and pelagic type. A large oil droplet was located in vitelline membrane of the fertilized egg. The egg envelopes have a single micropyle, which is thought to the pathway of sperm in the area of the animal pole. Specially, the micropyle of three-spot gourami was similar to that of marble gourami which is subspecies of three-spot gourami. An outer surface of the fertilized egg envelope was arranged by grooves in all three species. The fertilized egg envelopes consists of two distinct layers; an adhesive outer layer and an inner layer with high electron density. In conclusion, the morphological similarity of the fertilized egg, micropyle, outer surface and transverse section of the fertilized e9g envelope seems to be an indication of the Belontiidae.

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Ultrastructure of the Fertilized Egg Envelope from Hyphessobrycon serpae, Characidae, Teleost (경골어류 카라신과 Hyphessobrycon serpae의 수정란 난막 미세구조)

  • Kim, Dong-Heui;Deung, Young-Kun;Lee, Kyu-Jae
    • Applied Microscopy
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    • v.35 no.2
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    • pp.89-96
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    • 2005
  • The ultrastructures of the fertilized egg envelope from Hyphessobrycon serpae belonging to Characidae was studied using scanning and transmission electron microscopes to get systematic fundamental data for classification of species and to confirm whether micropyle is a common trait of Characidae or not. The fertilized egg was of colorless, transparent, spherical, adhesive and demersal type. There were not oil droplets in vitelline membrane and attached structures in the outside of fertilize egg envelope. The egg envelope had a single micropyle resembling the pathway of sperm in the area of the animal pole. The micropyle was surrounded by 13 to 15 protruded lines of the egg envelope in a radiated form. The outer surface of fertilized egg envelope was covered by reticular adhesive fibrous structures and irregularly arranged by pore canals. The fertilized egg envelope consisted of three distinct layers an outer adhesive fibrous layer with high electron density, a middle layer with pore canals, and an inner layer consisting of 6 to 7 lamellae alternating layers with interlamellae of lower electron density. These ultrastructural characters of fertilized egg envelope form Hyphessobrycon serpae can be utilized in taxonomy of teleost, and as fundamental data for study on early development of fertilized egg. It seems that the morphology of micropyle is a common trait of Characidae

A Study on the Development of an Oil Sweep System for Small Patrol Vessels (소형 경비함정 장착용 유흡착장비 개발에 관한 연구)

  • Lee, Si Chan;Kim, Hyung Gyu;Park, Yong Hee;Kim, Jong Ho;Lee, Yong Jun;Kim, Dae Il
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.24 no.2
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    • pp.282-287
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    • 2018
  • Due to limitations in responding to oil spill incidents that are urgent or that cover a wide area with only oil spill response vessels, Korea Coast Guard (KCG) patrol vessels are utilized. In this study, we have developed an oil sweep system suitable for small patrol vessels. The equipment consists of three components - fixed support, poles, and slide fixers - and can be fastened with a toggle pin to a fall prevention bar on small patrol vessels without welding or additional efforts for installation. The respective weights of each component - fixed support, poles (2 pcs), and slide fixers (4 pcs) - are approximately 9.2 kg, 6.5 kg, and 3.5 kg. The 3 m-pole, which is rotatable 180 degrees horizontally, is folded deckward when replacing oil absorbents. As the development of this equipment has improved the method of deploying and retrieving oil absorbents on KCG small patrol vessels, we expect that more efficient oil spill cleanup will be possible.

Validation of a New Design of Tellurium Dioxide-Irradiated Target

  • Fllaoui, Aziz;Ghamad, Younes;Zoubir, Brahim;Ayaz, Zinel Abidine;Morabiti, Aissam El;Amayoud, Hafid;Chakir, El Mahjoub
    • Nuclear Engineering and Technology
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    • v.48 no.5
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    • pp.1273-1279
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    • 2016
  • Production of iodine-131 by neutron activation of tellurium in tellurium dioxide ($TeO_2$) material requires a target that meets the safety requirements. In a radiopharmaceutical production unit, a new lid for a can was designed, which permits tight sealing of the target by using tungsten inert gaswelding. The leakage rate of all prepared targets was assessed using a helium mass spectrometer. The accepted leakage rate is ${\leq}10^{-4}mbr.L/s$, according to the approved safety report related to iodine-131 production in the TRIGA Mark II research reactor (TRIGA: Training, Research, Isotopes, General Atomics). To confirm the resistance of the new design to the irradiation conditions in the TRIGA Mark II research reactor's central thimble, a study of heat effect on the sealed targets for 7 hours in an oven was conducted and the leakage rates were evaluated. The results show that the tightness of the targets is ensured up to $600^{\circ}C$ with the appearance of deformations on lids beyond $450^{\circ}C$. The study of heat transfer through the target was conducted by adopting a one-dimensional approximation, under consideration of the three transfer modes-convection, conduction, and radiation. The quantities of heat generated by gamma and neutron heating were calculated by a validated computational model for the neutronic simulation of the TRIGA Mark II research reactor using the Monte Carlo N-Particle transport code. Using the heat transfer equations according to the three modes of heat transfer, the thermal study of I-131 production by irradiation of the target in the central thimble showed that the temperatures of materials do not exceed the corresponding melting points. To validate this new design, several targets have been irradiated in the central thimble according to a preplanned irradiation program, going from4 hours of irradiation at a power level of 0.5MWup to 35 hours (7 h/d for 5 days a week) at 1.5MW. The results showthat the irradiated targets are tight because no iodine-131 was released in the atmosphere of the reactor building and in the reactor cooling water of the primary circuit.

Interpretation of Airborne Magnetic and Radioactive Data for the Uranium Deposit in Geumsan Area (금산 함우라늄광상 분포지역의 항공자력/방사능 탐사자료 해석)

  • Shin, Eun-Ju;Ko, Kwangbeom;An, Dongkuk;Han, Kyeongsoo
    • Geophysics and Geophysical Exploration
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    • v.16 no.1
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    • pp.36-44
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    • 2013
  • We conducted the airborne magnetic and radiometric survey for the characterization of the black shale related and pyrometamorphic uranium deposits distributed in Geumsan area. For the successful characterization of the uranium deposits, the general geological and structural geological features were investigated based on the lithological and linear feature analysis to individual magnetic and radiometric data as the first step. Lithological analysis from the magnetic reduction to the pole and downward continuation map revealed that prominent positive anomalies caused by black and dark gray slate member were clearly recognized as magnetic sources. These results indicate that magnetic survey, even though it is not a direct method for the detection of uranium, can be a useful tool in uranium detection. By the linear feature analysis based on 2nd vertical derivative and curvature map, two linearments corresponded the gray hornfels and black slate member were extracted and in succession, the additional uranium potential zone was inferred. Final discrimination whether uranium-rich or not was confirmed by radiometric and uranium anomaly map. From these analysis, we finally concluded that uranium deposit originated by pyrometamorphic process was confined near the intrusive area only. On the contrary, it was found that black shale related uranium deposit is distributed and extended through out the entire survey area with south-west to north-east direction. In addition, from the linear feature analysis based on radiometric total anomaly map, the typical discontinuous characteristics were recognized in areas where uranium-contained linearments cross the faults. From the above discussion, we concluded that airborne magnetic and radiometric survey are complementary to each other. So it is preferable to carry out simultaneously for the efficient data processing and fruitful interpretation.

Landscape Changes Assessment of the National Road-Focused on the National Road in Suburb Areas- (국도의 경관변화 평가-교외지역 국도를 중심으로-)

  • 홍영록;권상준;조태동
    • Korean Journal of Environment and Ecology
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    • v.18 no.1
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    • pp.92-102
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    • 2004
  • In this study, landscape changes were examined by periods through the analysis of visual quantity on the landscape components of national road including the mountains, the sky, road pavements, street trees, paddy fields and dry fields, billboard, telegraph pole, building, structure, slope, guard fence, soundproof wall, and median strip etc. For this, subject national road in suburb arreas was selected to grasp its landscape characteristics, and divisional criteria of landscape type of the national road were suggested to look into the trend of landscape changes by periods. Findings of this study are as follows:1.Divisional criteria of landscape type of the national road include location and geometry, structure, land use, perspective, and surrounding buildings of the national road. 2.The highest visual quantity was found in road pavements by periods among landscape components of the national road. It was found that this result has a thread of connection with previous study emphasizing the importance of road geometry as one of landscape components, and road geometry shall be regarded as a visually significant component in analyzing and evaluating landscape of the national road. 3.Most distinguished factors of landscape changes of the national road by periods were median strip(F=33.296) and street tree(F=32.881), and then the sky(F=24.735) and mountain(F=23.477) showed similar level of statistically significant difference. However, statistically significance difference was not found in buildings and structures. 4.It was shown that natural elements decreased gradually but structural landscape made of artificial elements became the main in construction of the national road. This result implies that although the scenic and ecological soundness is recognized in construction of the national road, it is not out of the limitation of functionalities that is, access and mobility.

Neuroprotective Mechanism of Acupuncture at GB34 for Dopaminergic Neurons in the Striatum of a Parkinson's Disease Mouse Model (파킨슨병 동물 모델을 이용한 양릉천(GB34)의 선조체 내 도파민성신경세포 보호 기전 연구)

  • Jeon, Hyongjun;Yoo, Tae-Won;Kim, Dongsoo;Kwon, Sunoh;Kim, Seungtae
    • Korean Journal of Acupuncture
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    • v.32 no.3
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    • pp.108-115
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    • 2015
  • Objectives : Acupuncture is frequently used as an alternative therapy for Parkinson's disease(PD) in Korea. Using 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP)-induced Parkinson's disease mouse model, the present study investigated a possible role of acupuncture stimulation at GB34 in suppressing dopaminergic neuronal death and regulating the phosphorylation of protein kinase B(Akt) in substantia nigra(SN) and striatum(ST). Methods : Eight-week-old male C57BL/6 mice were administered intraperitoneally with 30 mg/kg of MPTP at 24-h intervals for 5 days. Acupuncture stimulation at GB34 or SI3 was performed once a day for 12 days consecutively from the first MPTP injection. After the last acupuncture stimulation, pole test was performed to assess the effect of the acupuncture stimulations. Dopaminergic neuronal survival in the SN and the ST, dopamine transporter( DAT) and caspase-3 expression in the ST were evaluated by immunohistochemistry. The phosphorylations of Akt in the SN and the ST were measured by Western blotting. Results : MPTP administration caused behavioral impairment and dopaminergic neuronal death in the SN and the ST. It also decreased DAT expression and increased caspase-3 expression in the ST. Acupuncture stimulation at GB34 alleviated these MPTP-induced impairments. Moreover, MPTP suppressed Akt phosphorylation in the SN and the ST, whereas acupuncture stimulation at GB34 alleviated the phosphorylation in the SN. Conclusions : These results indicate that acupuncture stimulation at GB34 can inhibit MPTP-induced dopaminergic neuronal death and alleviate the Akt phosphorylation in the SN, suggesting a possible role for acupuncture in the treatment of PD.

The Effects of Hiking Poles and Steady Walking Time on Up-hill Walking (폴 사용 여부와 걷는 지속시간 경과가 오르막 걷기에 미치는 영향)

  • Seo, Jung-Suk
    • Korean Journal of Applied Biomechanics
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    • v.19 no.2
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    • pp.227-235
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    • 2009
  • The purpose of this study was to analyze differences of 45minutes up-hill walking depending on hiking poles and steady walking time. Seven healthy men volunteered for this experiment. Each of them performed up-hill walking with hiking poles and without hiking poles at speed of 3.5km/h during 45minutes on a treadmill. The treadmill was set 25% up-hill inclination. The lower extremity 4 muscles activity including rectus femoris, tibialis anterior, gastrocnemius, biceps femoris was recorded and assessed by using EMG. And Heart rate(HR) and Rating of perceived exertion(RPE) were recorded and analyzed by 15minutes interval. The statistical analysis was two-way ANOVA with repeated measures to compare effects of hiking poles and steady walking time. The level of statistical significance for all tests was P<.05. The results of this study were following : Integrated EMG about four individual muscles doesn't have statistical significancy. However, the sum of IEMG of the four muscles was decreased some with poles than without poles(p<.0l) and IEMG about four muscles was rut different on steady walking time. Second, HR was increased significantly as time up(P<.01). RPE was decreased some with poles than without poles(P<.05) and RPE was increased significantly as time up(P<.01).

Geological Considerations on the Planning of the Corιervation of Stone-Cultural Properties in Korea (국내 석조문화재의 보전 대책수립을 위한 지질학적 고찰)

  • Lee, Sang Hun
    • Journal of Conservation Science
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    • v.7 no.1
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    • pp.9-18
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    • 1998
  • There are about 1470 stone-cultural properties in Korea which have been registered and protected by the government. Representative of them are pagoda, stupa, stone-buddha, stele support of banner pole and stone-lanterns. Most of them have been exposed to and weathered by natural environments. Stone-cultural properties are destroyed and/or destructed mostly by weathering and its related features of rocks. Therefore, it is necessary to conduct geological studies on the weathering phenomena and related features as well as characteristics of the rock itself must be done for the conservation. Thus geological investigation should include detail measurements, rock varieties and phases, structural lineaments of the rock, cause and degree of weathering, degree and patterns of crack development structural stability, identification of reusable parts for restoration, geomorphological characteristics of the site, and etc. The interaction among these factors must be investigated and analyzed, which must be used as basic data to establish the guideline of conservation and to plan for repair and/or restoration. The conservation plan should involve parts to be repaired, method of repair, type of cementing material, the characteristics of rock phases to be supplemented, method for maintaining structural stability, and method to delay or to prevent the rock weathering.

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