• 제목/요약/키워드: plant observation

검색결과 489건 처리시간 0.023초

파(Allium fistulosum L.)의 자방배양으로 부터 화아발생 및 꽃의 분화 (Flower Bud Induction and Flower Regeneration from Ovary Cultures of Allium fistulosum L.)

  • 김재훈;최용의;소웅영
    • 식물조직배양학회지
    • /
    • 제25권4호
    • /
    • pp.263-266
    • /
    • 1998
  • 파(Allium fistulosum L.)의 자방을 0.5mg/L NAA와 0.5mg/L BA 또는 0.5mg/L kinetin을 조합처리한 MS배지에서 배양하였을 때, 자방의 표면으로부터 화아발생 캘러스가 유도되었다. 화아발생 캘러스의 유지는 0.5mg/L NAA와 0.5mg/L kinetin이 첨가된 배지에서 계대배양 하였을 때 양호하였다. 배양 3-4주 후 화아들은 화아발생 캘러스로부터 형성되었지만, 계대배양을 거듭할수록 화아의 발생률이 감소하였다. 화아의 발생 단계별 조직학적 관찰 결과 돔형의 분열조직이 넓게 퍼지면서 자연상태의 꽃과 같이 외측에 화판, 내측에 심피와 배주가 기원된 후 성숙한 꽃으로 발달하였다.

  • PDF

Characterization of Sclerotinia sclerotiorum, an Emerging Fungal Pathogen Causing Blight in Hyacinth Bean (Lablab purpureus)

  • Prova, Ananya;Akanda, Abdul Mannan;Islam, Shaikhul;Hossain, Md. Motaher
    • The Plant Pathology Journal
    • /
    • 제34권5호
    • /
    • pp.367-380
    • /
    • 2018
  • Stems and pods of hyacinth bean cultivated in a farmer's field in Gazipur District, Bangladesh, were found rotted in nearly 5% hyacinth bean plants. A fungus having fluffy mycelium and large sclerotia was isolated from affected tissues. Combined results of morphological, molecular and pathological analyses identified the fungus as Sclerotinia sclerotiorum (Lib) de Bary. Inoculating the fungus on healthy hyacinth bean plants and pods reproduced the symptoms previously observed in the field. The three isolates obtained from naturally infected plants were cross inoculated in hyacinth bean, okra and African-American marigold and they were pathogenic to these hosts. The optimum temperature and pH for its growth were $20^{\circ}C$ and pH 5.0, respectively. Sclerotial development was favored at pH 5.0. Sucrose and mannitol were the best carbon sources to support hyphal growth, while glucose was the most favourable for sclerotial development. The hyacinth bean genotypes, HB-82 (Rupban Sheem) and HB-102 were found highly resistant, while HB-94 (Ashina) was moderate resistant to the fungus. Finally, S. sclerotiorum was sensitive to Bavistin, Dithane M-45 and Rovral fungicides and Ca in the form of $CaCl_2$. This observation could possibly aid in eliminating field loss in hyacinth bean caused by an emerging pathogenic fungus S. sclerotiorum.

Suppression of Melanose Caused by Diaporthe citri on Citrus Leaves Pretreated with Bio-sulfur

  • Shin, Yong Ho;Ko, Eun Ju;Kim, Su Jeong;Hyun, He Nam;Jeun, Yong Chull
    • The Plant Pathology Journal
    • /
    • 제35권5호
    • /
    • pp.417-424
    • /
    • 2019
  • Melanose, caused by Diaporthe citri, is one of severe diseases in citrus, a major economic resource in Jeju island. To reduce the usage amount of organic synthetic fungicide, bio-sulfur was tested as an alternative chemical to control citrus melanose in the present study. Direct antifungal activity of bio-sulfur against D. citri was determined through in vitro experiment using artificial nutrient media. Disease severity of melanose on bio-sulfur pretreated citrus leaves was lower than that on untreated ones. To illustrate the mechanism of disease suppression by bio-sulfur, infection structures were observed with a fluorescent microscope and a scanning electron microscope. In fluorescent microscopic observation, most conidia rarely germinated. In addition, hyphal growth on leaves pretreated with bio-sulfur was inhibited compared to that on untreated ones. In scanning electron microscope images of bio-sulfur pretreated leaves, surfaces of most conidia were shrunk while hyphae were morphologically changed and frequently branched. Such microscopic observations were also found for leaves pretreated with a commercial fungicide Dithianon. These results suggest that bio-sulfur may be used to control citrus melanose as an environment friendly alternative to organic synthetic fungicides

미나리 체세포 배발생과정의 해부학적 관찰 (Anatomical Observation of Somatic Embryogenesis in Oenanthe javanica ($B^{L}.$) DC.)

  • Gab Cheon KOH;Chang Soon AHN
    • 식물조직배양학회지
    • /
    • 제22권6호
    • /
    • pp.323-327
    • /
    • 1995
  • 미나리의 체세포 배발생 과정을 해부학적으로 구명하기 위하여 배발생 기원세포와 캘러스를 광학현미경 및 전자현미경으로 관찰한 결과, 배발생 세포는 hematoxylin에 짙은 보라색으로, 비배발생 세포는 safranin에 적색으로 염색되어 광학현미경하에서 쉽게 구별할 수 있었다. 배발생 캘러스는 많은 수의 원배 및 발육중인 배, 비배발생 세포 등으로 구성되어 있었다. 체세포 배발생은 발육 중인 배나 세포괴의 표피세포에 위치한 배발생 세포의 하나가 분열하거나 세포괴내의 비배발생 세포속에 묻혀 있는 배발생 세포가 분열하여 일어났다. 배발생 과정은 항상 일정한 형태는 아니지만 단세포로부터 일정한 segmentation 과정를 거쳐서 배발생이 진행되는 것으로 나타났다. 투과전자현미경에 의한 관찰에서 배발생 세포는 비배발생 세포에 비하여 세포질이 조밀하고 핵이 대형이며 amyloplast, 인지질체 및 세포소기 관들이 많으며 액포가 없거나 매우 작았다. 이들 세포들은 두터운 세포벽에 의하여 주위의 비배발생 세포와 분리되어 있으며 세포윤곽은 둥글었다. 주사전자현미경으로 관찰한 배발생캘러스는 외부가 그물이 씌워진 형태의 구형의 다양한 크기의 배들과 비교적 크기가 큰 비배발생 세포들이 혼재하였다. 한편 비배발생능 캘러스는 구성세포가 크고 외부에는 gelatin같은 물질로 덮여 있었다.

  • PDF

울돌목 조류에너지 개발 현황과 전망 (Development of Uldolmok Tidal Current Energy)

  • 이광수;염기대;박진순;강석구;박우선;한상훈;정공일;박정우
    • 한국신재생에너지학회:학술대회논문집
    • /
    • 한국신재생에너지학회 2005년도 춘계학술대회
    • /
    • pp.512-515
    • /
    • 2005
  • The Korean peninsula has a number of coastal sites where the rhythmic rising and lowering of water surface due to tides result in strong tidal current. The kinetic energy of these currents can be efficiently exploited by using tidal current turbines. The pilot tidal current power plant is to be constructed at the Uldolmok between Chindo and Haenam. Extensive coastal engineer ing research works have been carried out. This paper describes some observation results of field campaign, design of the supporting structure of a pilot plant of 1,000kW and a future tidal current power plant and so on.

  • PDF

울돌목 조류발전의 연안물리적 관점에서의 고찰 (Coastal-physical cceanographic aspects in relation to the tidal current power generation in the Uldolmok)

  • 염기대;이광수;박진순;강석구
    • 한국신재생에너지학회:학술대회논문집
    • /
    • 한국신재생에너지학회 2005년도 춘계학술대회
    • /
    • pp.516-519
    • /
    • 2005
  • The pilot tidal current power plant is to be constructed at the Uldolmok between Chindo and Haenam, during next year. and extensive coastal engineering research works have been carried out. In this paper we describes some observation results of the tide and tidal current. as well as modeling work in order to investigate the tide and tidal current regime change in relation to the tidal current power plant (TCPP) construction. The special modeling skill in order to consider the turbine operation in the TCPP is developed and applied to the estimation for the flow regime change by the simple layout of the tidal current power plant.

  • PDF

울돌목 조류발전의 연안물리적 관점에서의 고찰 (Coastal-physical cceanographic aspects in relation to the tidal current power generation in the Uldolmok)

  • 강석구;염기대;이광수;박진순
    • 신재생에너지
    • /
    • 제1권2호
    • /
    • pp.73-78
    • /
    • 2005
  • The pilot tidal current power plant is to be constructed at the Uldolmok between Chindo and Haenam, during next year, and extensive coastal engineering research works have been carried out. In this paper we describes some observation results of the tide and tidal current, as well as modeling work in order to investigate the tide and tidal current regime change In relation to the tidal current power plant [TCPP] construction. The special modeling skill in order to consider the turbine operation in the TCPP is developed and applied to the estimation for the flow regime change by the simple layout of the tidal current power plant.

  • PDF

내재성 기본모델을 사용한 적용제어 시스템의 구성 (Implementation of Implicit Model Reference Adaptive Control System)

  • 허욱열;고명삼
    • 대한전기학회논문지
    • /
    • 제32권4호
    • /
    • pp.136-144
    • /
    • 1983
  • In this paper, a new scheme of implicit MRAC is presented for single input single output discrete system. The MRAC can be applied to the nonminimum phase system, too. They have simple structure because the parameters of the controller are estimated directly by changing the plant output equation properly. In this scheme, the observation process is well seperated from the adaptation process, so the adaptation algorithm is derived from the exponentially weighted least square method which has fast convergence characteristics and can deal with the time varying plant. The consistency of the estimated parameter is proved. And it is also proved the whole system has the stabilizing property. The effectiveness of the algorithm and the structure is illustrated by the computer simulation of the model reference adaptive control for a third order plant. It is proposed how to select the selectable parameters in the adaptive control system from the simulation results.

  • PDF

Co-combustion of Bituminous Coal with Anthracite in a Down-firing, 200 MW Boiler

  • Park, Ho Young;Baek, Se Hyun;Kim, Young Joo;Kim, Tae Hyung;Kim, Hyun Hee;Lim, Hyun Soo;Park, Yoon Hwa
    • KEPCO Journal on Electric Power and Energy
    • /
    • 제1권1호
    • /
    • pp.93-97
    • /
    • 2015
  • The combustion tests for Korean anthracite-bituminous coal blend were carried out in the 200 MW utility boiler. The burning characteristics of the blend were studied with a thermogravimetric analyzer (TGA). From the observation of TGA burning profiles, it was found that the presence of bituminous coal in the blend appeared to enhance the reactivity of anthracite in the higher temperature region, indicating certain interactions between the two coals. The plant test showed the boiler operation was reasonably stable with somewhat poor combustion efficiency, and some modification of the combustion environment in the furnace is necessitate for the further stable plant operation.

유연 생산 시스템의 시뮬레이션을 위한 JR-Net 모델링 (Job Resource relation-Net Modeling for the Simulation of FMS)

  • 최병규;한관희
    • 산업공학
    • /
    • 제8권3호
    • /
    • pp.61-73
    • /
    • 1995
  • As the level of maunfacturing system automation increases, the issues of modeling and simulation of AMS(Automated Manufacturing System) are becoming more important. Proposed in this paper is the JR-Net(Job Resource relation-Net) modeling framework which naturally mimics the process of designing an AMS by FA(Factory Automation) engineers. Its main purpose is to provide a modeling tool which facilitate modeling work of AMS for FA engineers unfamiliar with simulation modeling. The proposed modeling scheme is based on the extensive observation that typical AMSs are built from the set of 'standard' components(or catalog items). As an application of the proposed model, two real examples of FMS('G7'FMS model plant, RPI FMS) are modeled by JR-Net, and in case of FMS model plant, a simulation program development procedure using JR-Net modeling results is explained. Finally, simulation result of FMS model plant is analyzed.

  • PDF