Korean Journal of Plant Tissue Culture (식물조직배양학회지)

The Korean Society of Plant Biotechnology (한국식물생명공학회)
권호 표지

Flower Bud Induction and Flower Regeneration from Ovary Cultures of Allium fistulosum L.

파(Allium fistulosum L.)의 자방배양으로 부터 화아발생 및 꽃의 분화

  • 김재훈 (생명공학연구소 식물생화학 Research Unit) ;
  • 최용의 (전북대학교 자연과학대학 생물과학부) ;
  • 소웅영 (전북대학교 자연과학대학 생물과학부)
  • Published : 1998.07.01
Download PDF
⟨ Previous Next ⟩

Abstract

Flowergenic callus was induced from the ovary surface of Allium fistulosum L. cultured on MS medium containing 0.5mg/L NAA and 0.5mg/L BA or 0.5mg/L kinetin. After 3-4 weeks of culture, the flower buds were developed from flowergenic callus. The continuous production of flowergenic callus was proliferated, when subcultured on the medium containing 0.5mg/L NAA and 0.5mg/L kinetin. However, frequency of flower bud formation from flowergenic callus was decreased as the subculture was repeated. Histological observation reveals that the developmental pattern of flower bud from flowergenic callus was closely similar to that of natural flowers.

파(Allium fistulosum L.)의 자방을 0.5mg/L NAA와 0.5mg/L BA 또는 0.5mg/L kinetin을 조합처리한 MS배지에서 배양하였을 때, 자방의 표면으로부터 화아발생 캘러스가 유도되었다. 화아발생 캘러스의 유지는 0.5mg/L NAA와 0.5mg/L kinetin이 첨가된 배지에서 계대배양 하였을 때 양호하였다. 배양 3-4주 후 화아들은 화아발생 캘러스로부터 형성되었지만, 계대배양을 거듭할수록 화아의 발생률이 감소하였다. 화아의 발생 단계별 조직학적 관찰 결과 돔형의 분열조직이 넓게 퍼지면서 자연상태의 꽃과 같이 외측에 화판, 내측에 심피와 배주가 기원된 후 성숙한 꽃으로 발달하였다.

Keywords

References

  1. Proceedings of the Royal Society Series B v.190 Chaila MKH;Aksenova NP;Konstantinova TN;Baverina TV
  2. Bot Gaz v.141 The regulation of tobacco floral organ initiation Hughen AM
  3. Phytomorphology v.12 In vitro flowering of Crepis capillaris Jayakar M
  4. Planta v.181 Further experiments of wisconsin 38 tobacco Kaur-Sawhney R;Kandpal G;McGonigle B;Galston AW
  5. Planta v.173 Spermidine and flower bud differntiation in thin-layer tobacco tissue cultures Kaur-Sawhney R;Tiburcio AF;Galsto AW
  6. Korean J Plant Tissue Culture v.20 Somatic embryogenesis in froral organ cultures of Allium fistulosum L Kim JW;Soh YW
  7. Plana Sci v.114 Plant regeneration through somatic embryogenesis from supension culture of Allium fistulosum L Lim JW;Soh YW
  8. Plant Cell Physiol v.32 In vitro flowweing of genseng (Panax ginseng C.A. Meyer) zygotic embryos induced by growth regulators Lee HS;Lee KW;Yang SG;Liu JR
  9. Planta v.180 In vitro flower formation in leaf explants of tomato; effect of NaCl Lui KB;Li SX
  10. Planta v.185 Floral determination and in vitro floral differentiation in isolated shoot apices of Lolium temulentum L McDaniel CN;King RW;Evans LT
  11. Physiol Planta v.15 A revised medium for repid growth and bio assays with tobacco tissue cultures Murashige T;Skoog F
  12. Theoretical and Applied Genetics v.69 In vitro flowering and pod formation from cotyledons of groundnut (Arachis hypogaea L.) Narasimhuiu SB;Reddy GM
  13. Annals of Botany v.52 Flower formation in vitro by hypocotyl explants of cucumber (cucumis sativus L.) Rajasekaran K;Mullins MG;Nair Y
  14. Phytomorphology v.12 In vitro behaviour of inflorescence segments of Mazuspumilus Raste AP;Ganapathy PS
  15. Plant Cell Physiol v.23 The role of cotyledons in flower initiation of Pharbitis nil at low temperatures Shinozaki M;Takimoto A
  16. Planta v.180 The dose of 1-naphthaleneacetic acid determines flower-bud regeneration in tobacco explants at a large range of concentrations Smulders MJM;Visser EJW;Croes AF;Wullems GJ
  17. Planta Cell Physiol v.22 Effects of IAA, zeatin, ammonium nitrate and sucrose on the initiation and development of floral buds in Torenia stem segments cultured in vitro Tanimoto S;Harada H
  18. Plant Cell Physiol v.29 Polyamine biosynthesis during vegetative and floral bud differentiation in thin layer tobacco tissue culture Tiburcio AF;Kaur-Sawhney R;Galston AW
  19. Planta v.115 Direct flower neoformation from superficial tissue of small explants of Nicotiana tabacum L Tran Thanh Van M
  20. Physiol Plant v.74 Uptake and metabolism of benzyladenine in the early stage of flower bud development in vitro in tobacco Vander Krieken WM;Croes AF;Barendse GMW;Wullems GJ
  21. Plant Physiol v.44 Flower formation in excised tobacco stem segments;I. Methodology and effects of plant hormones Wardell WL;Stoog F