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http://dx.doi.org/10.5423/PPJ.OA.03.2019.0067

Suppression of Melanose Caused by Diaporthe citri on Citrus Leaves Pretreated with Bio-sulfur  

Shin, Yong Ho (College of Applied Life Science, Major of Plant Resources & Environment, Jeju National University)
Ko, Eun Ju (College of Applied Life Science, Major of Plant Resources & Environment, Jeju National University)
Kim, Su Jeong (Postech Biotech Center, Pohang University of Science and Technology)
Hyun, He Nam (College of Applied Life Science, Major of Plant Resources & Environment, Jeju National University)
Jeun, Yong Chull (College of Applied Life Science, Major of Plant Resources & Environment, Jeju National University)
Publication Information
The Plant Pathology Journal / v.35, no.5, 2019 , pp. 417-424 More about this Journal
Abstract
Melanose, caused by Diaporthe citri, is one of severe diseases in citrus, a major economic resource in Jeju island. To reduce the usage amount of organic synthetic fungicide, bio-sulfur was tested as an alternative chemical to control citrus melanose in the present study. Direct antifungal activity of bio-sulfur against D. citri was determined through in vitro experiment using artificial nutrient media. Disease severity of melanose on bio-sulfur pretreated citrus leaves was lower than that on untreated ones. To illustrate the mechanism of disease suppression by bio-sulfur, infection structures were observed with a fluorescent microscope and a scanning electron microscope. In fluorescent microscopic observation, most conidia rarely germinated. In addition, hyphal growth on leaves pretreated with bio-sulfur was inhibited compared to that on untreated ones. In scanning electron microscope images of bio-sulfur pretreated leaves, surfaces of most conidia were shrunk while hyphae were morphologically changed and frequently branched. Such microscopic observations were also found for leaves pretreated with a commercial fungicide Dithianon. These results suggest that bio-sulfur may be used to control citrus melanose as an environment friendly alternative to organic synthetic fungicides
Keywords
environment friendly; fluorescence microscope; infection behavior; scanning electron microscope;
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Times Cited By KSCI : 12  (Citation Analysis)
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