• Title/Summary/Keyword: plant observation

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Flower Bud Induction and Flower Regeneration from Ovary Cultures of Allium fistulosum L. (파(Allium fistulosum L.)의 자방배양으로 부터 화아발생 및 꽃의 분화)

  • 김재훈;최용의;소웅영
    • Korean Journal of Plant Tissue Culture
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    • v.25 no.4
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    • pp.263-266
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    • 1998
  • Flowergenic callus was induced from the ovary surface of Allium fistulosum L. cultured on MS medium containing 0.5mg/L NAA and 0.5mg/L BA or 0.5mg/L kinetin. After 3-4 weeks of culture, the flower buds were developed from flowergenic callus. The continuous production of flowergenic callus was proliferated, when subcultured on the medium containing 0.5mg/L NAA and 0.5mg/L kinetin. However, frequency of flower bud formation from flowergenic callus was decreased as the subculture was repeated. Histological observation reveals that the developmental pattern of flower bud from flowergenic callus was closely similar to that of natural flowers.

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Characterization of Sclerotinia sclerotiorum, an Emerging Fungal Pathogen Causing Blight in Hyacinth Bean (Lablab purpureus)

  • Prova, Ananya;Akanda, Abdul Mannan;Islam, Shaikhul;Hossain, Md. Motaher
    • The Plant Pathology Journal
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    • v.34 no.5
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    • pp.367-380
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    • 2018
  • Stems and pods of hyacinth bean cultivated in a farmer's field in Gazipur District, Bangladesh, were found rotted in nearly 5% hyacinth bean plants. A fungus having fluffy mycelium and large sclerotia was isolated from affected tissues. Combined results of morphological, molecular and pathological analyses identified the fungus as Sclerotinia sclerotiorum (Lib) de Bary. Inoculating the fungus on healthy hyacinth bean plants and pods reproduced the symptoms previously observed in the field. The three isolates obtained from naturally infected plants were cross inoculated in hyacinth bean, okra and African-American marigold and they were pathogenic to these hosts. The optimum temperature and pH for its growth were $20^{\circ}C$ and pH 5.0, respectively. Sclerotial development was favored at pH 5.0. Sucrose and mannitol were the best carbon sources to support hyphal growth, while glucose was the most favourable for sclerotial development. The hyacinth bean genotypes, HB-82 (Rupban Sheem) and HB-102 were found highly resistant, while HB-94 (Ashina) was moderate resistant to the fungus. Finally, S. sclerotiorum was sensitive to Bavistin, Dithane M-45 and Rovral fungicides and Ca in the form of $CaCl_2$. This observation could possibly aid in eliminating field loss in hyacinth bean caused by an emerging pathogenic fungus S. sclerotiorum.

Suppression of Melanose Caused by Diaporthe citri on Citrus Leaves Pretreated with Bio-sulfur

  • Shin, Yong Ho;Ko, Eun Ju;Kim, Su Jeong;Hyun, He Nam;Jeun, Yong Chull
    • The Plant Pathology Journal
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    • v.35 no.5
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    • pp.417-424
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    • 2019
  • Melanose, caused by Diaporthe citri, is one of severe diseases in citrus, a major economic resource in Jeju island. To reduce the usage amount of organic synthetic fungicide, bio-sulfur was tested as an alternative chemical to control citrus melanose in the present study. Direct antifungal activity of bio-sulfur against D. citri was determined through in vitro experiment using artificial nutrient media. Disease severity of melanose on bio-sulfur pretreated citrus leaves was lower than that on untreated ones. To illustrate the mechanism of disease suppression by bio-sulfur, infection structures were observed with a fluorescent microscope and a scanning electron microscope. In fluorescent microscopic observation, most conidia rarely germinated. In addition, hyphal growth on leaves pretreated with bio-sulfur was inhibited compared to that on untreated ones. In scanning electron microscope images of bio-sulfur pretreated leaves, surfaces of most conidia were shrunk while hyphae were morphologically changed and frequently branched. Such microscopic observations were also found for leaves pretreated with a commercial fungicide Dithianon. These results suggest that bio-sulfur may be used to control citrus melanose as an environment friendly alternative to organic synthetic fungicides

Anatomical Observation of Somatic Embryogenesis in Oenanthe javanica ($B^{L}.$) DC. (미나리 체세포 배발생과정의 해부학적 관찰)

  • Gab Cheon KOH;Chang Soon AHN
    • Korean Journal of Plant Tissue Culture
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    • v.22 no.6
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    • pp.323-327
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    • 1995
  • This experiment was carried out to observe the origin and developmental pattern of somatic embryos of Oenanthe javanica ($B^{L}.$) DC. The experiment included observation of embryogenic cells and their development stages by light microscope, transmission electron microscope and scanning electron microscope. The embryogenic cells, which were smaller than non-embryogenic cells in size with expanded nucleus and dense cytoplasm. When stained with hematoxylin, the embryogenic cells were readily distinguished from the non-embryogenic cells of which cell walls were stained with safranin. It was observed at somatic embryos developed from single cells on the epidermis of developing embryos or in the surface or inside of embryogenic clumps by segmentation pattern. Observation with a transmission electron microscope revealed that the embryogenic cells had dense cytoplasm expanded nucleus, small vacuoles, large amyloplasts containing starch grains, and abundant organelles including lipid bodies. Under a scanning electron microscope, embryogenic callus was shown to consist of very smaller cells than non-embryogenic cells in an orderly arrangement and covered with a net-like structure, while the non-embryogenic callus consisted of large cells, irregular in size and arrangement, and covered with a gelatin-like material.

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Development of Uldolmok Tidal Current Energy (울돌목 조류에너지 개발 현황과 전망)

  • Lee Kwang-Soo;Yum Ki-Dai;Park Jin Soon;Kang Sok Kuh;Park Woo-Sun;Han Sang-Hun;Jung Gong-Il;Park Jung Woo
    • 한국신재생에너지학회:학술대회논문집
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    • 2005.06a
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    • pp.512-515
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    • 2005
  • The Korean peninsula has a number of coastal sites where the rhythmic rising and lowering of water surface due to tides result in strong tidal current. The kinetic energy of these currents can be efficiently exploited by using tidal current turbines. The pilot tidal current power plant is to be constructed at the Uldolmok between Chindo and Haenam. Extensive coastal engineer ing research works have been carried out. This paper describes some observation results of field campaign, design of the supporting structure of a pilot plant of 1,000kW and a future tidal current power plant and so on.

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Coastal-physical cceanographic aspects in relation to the tidal current power generation in the Uldolmok (울돌목 조류발전의 연안물리적 관점에서의 고찰)

  • Yum Ki-Dai;Lee Kwang Soo;Park Jin Soon;Kang Sok Kuh
    • 한국신재생에너지학회:학술대회논문집
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    • 2005.06a
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    • pp.516-519
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    • 2005
  • The pilot tidal current power plant is to be constructed at the Uldolmok between Chindo and Haenam, during next year. and extensive coastal engineering research works have been carried out. In this paper we describes some observation results of the tide and tidal current. as well as modeling work in order to investigate the tide and tidal current regime change in relation to the tidal current power plant (TCPP) construction. The special modeling skill in order to consider the turbine operation in the TCPP is developed and applied to the estimation for the flow regime change by the simple layout of the tidal current power plant.

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Coastal-physical cceanographic aspects in relation to the tidal current power generation in the Uldolmok (울돌목 조류발전의 연안물리적 관점에서의 고찰)

  • Kang Sok Kuh;Yum Ki-Dai;Lee Kwang Soo;Park Jin Soon
    • New & Renewable Energy
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    • v.1 no.2 s.2
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    • pp.73-78
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    • 2005
  • The pilot tidal current power plant is to be constructed at the Uldolmok between Chindo and Haenam, during next year, and extensive coastal engineering research works have been carried out. In this paper we describes some observation results of the tide and tidal current, as well as modeling work in order to investigate the tide and tidal current regime change In relation to the tidal current power plant [TCPP] construction. The special modeling skill in order to consider the turbine operation in the TCPP is developed and applied to the estimation for the flow regime change by the simple layout of the tidal current power plant.

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Implementation of Implicit Model Reference Adaptive Control System (내재성 기본모델을 사용한 적용제어 시스템의 구성)

  • 허욱열;고명삼
    • The Transactions of the Korean Institute of Electrical Engineers
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    • v.32 no.4
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    • pp.136-144
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    • 1983
  • In this paper, a new scheme of implicit MRAC is presented for single input single output discrete system. The MRAC can be applied to the nonminimum phase system, too. They have simple structure because the parameters of the controller are estimated directly by changing the plant output equation properly. In this scheme, the observation process is well seperated from the adaptation process, so the adaptation algorithm is derived from the exponentially weighted least square method which has fast convergence characteristics and can deal with the time varying plant. The consistency of the estimated parameter is proved. And it is also proved the whole system has the stabilizing property. The effectiveness of the algorithm and the structure is illustrated by the computer simulation of the model reference adaptive control for a third order plant. It is proposed how to select the selectable parameters in the adaptive control system from the simulation results.

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Co-combustion of Bituminous Coal with Anthracite in a Down-firing, 200 MW Boiler

  • Park, Ho Young;Baek, Se Hyun;Kim, Young Joo;Kim, Tae Hyung;Kim, Hyun Hee;Lim, Hyun Soo;Park, Yoon Hwa
    • KEPCO Journal on Electric Power and Energy
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    • v.1 no.1
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    • pp.93-97
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    • 2015
  • The combustion tests for Korean anthracite-bituminous coal blend were carried out in the 200 MW utility boiler. The burning characteristics of the blend were studied with a thermogravimetric analyzer (TGA). From the observation of TGA burning profiles, it was found that the presence of bituminous coal in the blend appeared to enhance the reactivity of anthracite in the higher temperature region, indicating certain interactions between the two coals. The plant test showed the boiler operation was reasonably stable with somewhat poor combustion efficiency, and some modification of the combustion environment in the furnace is necessitate for the further stable plant operation.

Job Resource relation-Net Modeling for the Simulation of FMS (유연 생산 시스템의 시뮬레이션을 위한 JR-Net 모델링)

  • Choi, Byoung-Kyu;Han, Kwan-Hee
    • IE interfaces
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    • v.8 no.3
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    • pp.61-73
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    • 1995
  • As the level of maunfacturing system automation increases, the issues of modeling and simulation of AMS(Automated Manufacturing System) are becoming more important. Proposed in this paper is the JR-Net(Job Resource relation-Net) modeling framework which naturally mimics the process of designing an AMS by FA(Factory Automation) engineers. Its main purpose is to provide a modeling tool which facilitate modeling work of AMS for FA engineers unfamiliar with simulation modeling. The proposed modeling scheme is based on the extensive observation that typical AMSs are built from the set of 'standard' components(or catalog items). As an application of the proposed model, two real examples of FMS('G7'FMS model plant, RPI FMS) are modeled by JR-Net, and in case of FMS model plant, a simulation program development procedure using JR-Net modeling results is explained. Finally, simulation result of FMS model plant is analyzed.

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