• Journal of Microbiology and Biotechnology
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• v.16 no.1
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• pp.84-91
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• 2006

A Saccharomyces cerevisiae pgs1 nulI mutant, which is deficient with phosphatidyl glycerol (PG) and cardiolipin (CL) biosynthesis, grows well on most fermentable carbon sources, but fails to grow on non-fermentable carbon sources such as glycerol, ethanol, and lactate. This mutant also cannot grow on galactose medium as the sole carbon source. We found that the incorporation of $[^{14}C]-galactose$, which is the first step of the galactose metabolic pathway (Leloir pathway), into the pgs 1 null mutant cell was extremely repressed. Exogenously expressed PGS1 (YCpPGS1) under indigenous promoter could completely restore the pgs1 growth defect on non-fermentable carbon sources, and dramatically recovered $[^{14}C]-galactose$ incorporation into the pgs1 mutant cell. However, PGS1 expression under the GALl promoter $(YEpP_{GAL1}-PGS1myc)$ could not complement pgs1 mutation, and the GAL2-lacZ fusion gene $(YEpP_{GAL2}-lacZ)$ also did not exhibit its $\beta-galactosidase$ activity in the pgs1 mutant. In wild-type yeast, antimycin $A(1\;{\mu}g/ml)$, which inhibits mitochondrial complex III, severely repressed not only the expression of the GAL2-lacZ fusion gene, but also uptake of $[^{14}C]-galactose$. However, exogenously expressed PGS1 partially relieved these inhibitory effects of antimycin A in both the pgs1 mutant and wild-type yeast, although it could not basically restore the growth defect on galactose by antimycin A. These results suggest that the PGSI gene product has an important role in utilization of galactose by Gal genes, and that intact mitochondrial function with PGS1 should be required for galactose incorporation into the Leloir pathway. The PGS1 gene might provide a clue to resolve the historic issue about the incapability of galactose with deteriorated mitochondrial function.

• Journal of the Korean Society of Manufacturing Technology Engineers
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• v.25 no.1
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• pp.21-29
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• 2016

To overcome the large ring gear manufacturing problems seen in slewing bearings and girth gears, pin gear drive units have been developed. Among them, a novel slewing bearing with an internal pinwheel gear set (i-PGS) is introduced in this paper. First, we consider the exact cam pinion profile of i-PGS with the introduction of a profile shift coefficient. Furthermore, a new root relief profile modification for the i-PGS cam pinion is presented. Then, the contact stresses are investigated to determine the characteristics of the surface fatigue by varying the shape design parameters. The results show that the contact stresses of i-PGS can be reduced significantly by increasing the profile shift coefficient. In addition, the contact ratio, a measure of teeth overlapping action, decreases with the decrease of the allowable pressure angle.

• BMB Reports
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• v.42 no.12
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• pp.769-775
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• 2009

Periplasmic glucans (PGs) are general constituents in the periplasmic space of Proteobacteria. PGs from bacterial strains are found in larger amounts during growth on medium with low osmolarity and thus are often been specified as osmoregulated periplasmic glucans (OPGs). Furthermore, they appear to play crucial roles in pathogenesis and symbiosis. PGs have been classified into four families based on the structural features of their backbones, and they can be modified by a variety of non-sugar substituents. It has also recently been confirmed that novel PGs with various degrees of polymerization (DPs) and/or different substituents are produced under different growth conditions among Proteobacteria. In addition to their biological functions as regulators of low osmolarity, PGs have a variety of physico-chemical properties due to their inherent three-dimensional structures, hydrogen-bonding and complex-forming abilities. Thus, much attention has recently been focused on their physico-chemical applications. In this review, we provide an updated classification of PGs, as well as a description of the occurrences of novel PGs with substituents under various bacterial growth environments, the genes involved in PG biosynthesis and the various physico-chemical properties of PGs.

• Journal of the Korean Institute of Landscape Architecture
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• v.38 no.5_2
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• pp.151-162
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• 2010

This research aimed at measuring Park and Green Satisfaction (PGS) using subjective indicators of 'surface, line and spot' green evaluated by citizens. Also frequency of visits to park and green measured using objective indicators (number of visits) to find the relationship with PGS. A conceptual model of PGS was developed to relate evaluation to satisfaction and finally to utilization of open spaces. A sample of 500 questionnaire survey was employed for Uijeongbu City in Korea. A Structual Equation Modeling (AMOS) techniques was used to test the hypothesized relationship among factors (construct). As a result, first, PGS was explained by three latent factors of 'urban park' (${\gamma}=0.54$), 'linear facilities' (${\gamma}=0.25$), and 'surface green' (${\gamma}=0.15$) respectively. These three exogenous construct was found very useful classification system for open spaces of cities. Second, PGS (${\gamma}=0.34$) was found as a mediating variable to utilization of open spaces and also PGS was closely related to citizens Environmental Quality Satisfaction (EQS), such concept as, 'livability' and 'aesthetic quality'. The more satisfied with park and green the more people use the space. The PGS was an important QOL indicator together with the subjective indicator of 'livability'. Third, jogging and walking trails and bike ways along the river corridor was the most important green facilities contribute to the PGS and EQS. The near the distance (within 500m) the more number of visit to river corridor (green way). The river corridor promote accessibility to nature and other parks.

• Journal of Microbiology and Biotechnology
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• v.26 no.2
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• pp.421-431
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• 2016

Recently, poly-γ-glutamic acid synthetase A (pgsA) has been applied to display exogenous proteins on the surface of Lactobacillus casei or Lactococcus lactis, which results in a surface-displayed component of bacteria. However, the ability of carrying genes encoded by plasmids and the expression efficiency of recombinant bacteria can be somewhat affected by the longer gene length of pgsA (1,143 bp); therefore, a truncated gene, pgsA, was generated based on the characteristics of pgsA by computational analysis. Using murine IL-10 as an exogenous gene, recombinant Lactobacillus plantarum was constructed and the capacity of the surface-displayed protein and functional differences between exogenous proteins expressed by these strains were evaluated. Surface expression of IL-10 on both recombinant bacteria with anchorins and the higher expression levels in L. plantarum-pgsA'-IL-10 were confirmed by western blot assay. Most importantly, up-regulation of IL-1β, IL-6, TNF-α, IFN-γ, and the nuclear transcription factor NF-κB p65 in RAW264.7 cells after stimulation with Poly(I:C) or LPS was exacerbated after co-culture with L. plantarum-pgsA. By contrast, IL-10 expressed by these recombinant strains could reduce these factors, and the expression of these factors was associated with recombinant strains that expressed anchorin (especially in L. plantarum-pgsA'-IL-10) and was significantly lower compared with the anchorin-free strains. These findings indicated that exogenous proteins could be successfully displayed on the surface of L. plantarum by pgsA or pgsA', and the expression of recombinant bacteria with pgsA' was superior compared with bacteria with pgsA.

• Journal of Advanced Marine Engineering and Technology
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• v.33 no.1
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• pp.159-165
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• 2009

In this study, using a periodical ground structure(PGS) on GaAs monolithic microwave integrated circuit(MMIC), transmission line with a high isolation characteristic was developed for application to compact signal/bias lines of highly integrated MMIC. And the origin of the high isolation characteristic was theoretically investigated. The high isolation characteristic was originated from a resonance between adjacent microstrip lines employing PGS. With only a spacing of $20{\mu}m$, the coupled microstrip line employing PGS showed an isolation value of -47 dB at 60 GHz. The frequency range for high isolation was easily controlled by changing the PGS structure. Above results indicate that microstrip lines employing PGS are very useful for application to compact signal/bias lines of highly integrated MMIC requiring a high isolation characteristics between lines. In addition, equivalent circuit employing closed-form equation for the coupled line with PGS was also extracted.

• YAKHAK HOEJI
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• v.26 no.4
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• pp.209-214
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• 1982

Acute toxicities of purified ginseng saponin (PGS) in mice, and sbacute toxicities of PGS in rats were investigated. Average lethal doses ($LD_{50}$) of PGS in male mice were 270mg/kg (i.v.), 342mg/kg (i.p.), 505mg/kg (i.m.), 950mg/kg (s.c.), and more than 5,000mg/kg (p.o.), respectively. Results of subacute toxicity of PGS was as follows. Body weight was markedly increased by administration of PGS 7.7mg/kg but side effects were shown by administration of 77mg/kg and above dose. Especially administration of PGS 240mg/kg caused a marked decrease of albumin/globulin ratio, and 28% increase of urea nitrogen in serum, as well as 33% increase of liver weight/body weight ratio.

• Journal of Biosystems Engineering
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• v.41 no.3
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• pp.145-152
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• 2016

Purpose: This study aims to establish the effect of pinhole position errors in the planet carrier of a planetary gear set (PGS) on load sharing among the planet gears in the hydromechanical transmission (HMT) system of an agricultural tractor. Methods: A simulation model of a PGS with five planet gears was developed to analyze load sharing among the planet gears. The simulation model was verified by comparing i ts r esults w ith those of a model developed in a previous s tudy. The verified simulation model was used to analyze the load-sharing characteristics of the planet gears with respect to the pinhole position error and the input torque to the PGS. Results: Both simulation models had identical load magnitude sequences for the five planet gears. However, the load magnitudes on the corresponding planet gears differed between the models because of the different stiffnesses of the PGS components and the input torques to the PGS. The verified simulation model demonstrated that the evenness of load sharing among the planet gears increases with decreasing pinhole position error and increasing input torque. Conclusions: The geometrical tolerance of the pinhole position should be properly considered during the design of the planet carrier to improve the service life of the PGS and load sharing among the planet gears.

• Proceedings of the KIEE Conference
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• 2007.07a
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• pp.272-273
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• 2007

This paper presents the characteristic of rectangular and octagonal spiral inductor using PGS(Patterned Ground Shield). We investigated variation of inductance and Q-factor with changing of turn number at fixed width, spacing and inner diameter. We confirmed that characteristic of inductance and Q-factor be appled PGS in rectangular and octagonal types spiral inductor by EM simulation tool. Inductance decreased irrespective of structure but Q-factor increased. When PGS not exist, Q-factor of Inductor is analogous at classification frequency but, rectangular is a few larger then octagonal in small turn number. The other side, When PGS is inserted, we confirmed that octagonal lager then rectangular in many turn number. Q-factor is improved in case of octagonal structure and small turn number by PGS effect.

• The Plant Pathology Journal
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• v.29 no.3
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• pp.234-241
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• 2013

Polygalacturonase (PG) gene is a typical gene family present in eukaryotes. Forty-nine PGs were mined from the genomes of Neurospora crassa and five Aspergillus species. The PGs were classified into 3 clades such as clade 1 for rhamno-PGs, clade 2 for exo-PGs and clade 3 for exo- and endo-PGs, which were further grouped into 13 sub-clades based on the polypeptide sequence similarity. In gene structure analysis, a total of 124 introns were present in 44 genes and five genes lacked introns to give an average of 2.5 introns per gene. Intron phase distribution was 64.5% for phase 0, 21.8% for phase 1, and 13.7% for phase 2, respectively. The introns varied in their sequences and their lengths ranged from 20 bp to 424 bp with an average of 65.9 bp, which is approximately half the size of introns in other fungal genes. There were 29 homologous intron blocks and 26 of those were sub-clade specific. Intron losses were counted in 18 introns in which no obvious phase preference for intron loss was observed. Eighteen introns were placed at novel positions, which is considerably higher than those of plant PGs. In an evolutionary sense both intron loss and gain must have taken place for shaping the current PGs in these fungi. Together with the small intron size, low conservation of homologous intron blocks and higher number of novel introns, PGs of fungal species seem to have recently undergone highly dynamic evolution.