• Title/Summary/Keyword: pesticide resistance

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The Evaluation of Thermal Properties of Pesticide Protective Clothing using the Surface Temperature Controlled Thermal Manikin (열 마네킹을 이용한 부직포 농약 방호복의 열적 성능 평가)

  • Choi, Jong-Myoung
    • Korean Journal of Human Ecology
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    • v.7 no.1
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    • pp.119-127
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    • 1998
  • The purpose of this study was to evaluate thermal properties of pesticide protective clothing made of three different nonwoven fabrics which have barrier properties of pesticide. In order to assay the thermal properties of experimental clothing, thermal resistance measurements for clo value and thermographic assessment were conducted using a surface temperature controlled thermal manikin. The thermal manikin was dressed with underwear and experimental clothing. Air temperature in a climate chamber was kept at $28^{\circ}C$ and its humidity was 70% RH. Air velocity was controlled at less than 0.15m/s. Inner radient temperature was almost equal to the air temperature. The basic thermal insulation value(Icl) of underwear was 0.28 clo. The thermal properties of the experimental clothing were varied according to the type of material used in construction. The basic clothing insulation value for C1(spunbonded nonwoven fabric), C2(spunlaced nonwoven fabric), C3(SMS nonwoven fabric) were 0.705 clo, 0.725 clo, 0.738 clo respectively. The C3 experimental clothing made of SMS resulted in higher surface temperatures than the others with more yellowing spots being evident on the thermogram.

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Evaluation of Antimicrobial Properties of Lichen Substances against Plant Pathogens

  • Paguirigan, Jaycee A.;Liu, Rundong;Im, Seong Mi;Hur, Jae-Seoun;Kim, Wonyong
    • The Plant Pathology Journal
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    • v.38 no.1
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    • pp.25-32
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    • 2022
  • Plant pathogens pose major threats on agriculture and horticulture, causing significant economic loss worldwide. Due to the continuous and excessive use of synthetic pesticides, emergence of pesticide resistant pathogens has become more frequent. Thus, there is a growing needs for environmentally-friendly and selective antimicrobial agents with a novel mode of action, which may be used in combination with conventional pesticides to delay development of pesticide resistance. In this study, we evaluated the potentials of lichen substances as novel biopesticides against eight bacterial and twelve fungal plant pathogens that have historically caused significant phytopathological problems in South Korea. Eight lichen substances of diverse chemical origins were extracted from axenic culture or dried specimen, and further purified for comparative analysis of their antimicrobial properties. Usnic acid and vulpinic acid exhibited strong antibacterial activities against Clavibacter michiganensis subsp. michiganensis. In addition, usnic acid and vulpinic acid were highly effective in the growth inhibition of fungal pathogens, such as Diaporthe eres, D. actinidiae, and Sclerotinia sclerotiorum. Intriguingly, the growth of Rhizoctonia solani was specifically inhibited by lecanoric acid, indicating that lichen substances exhibit some degrees of selectivity to plant pathogens. These results suggested that lichen substance can be used as a selective biopesticide for controlling plant disease of agricultural and horticultural significance, minimizing possible emergence of pesticide resistant pathogens in fields.

Analysis of Pyrethroid Resistance Allele in Malaria Vector Anopheles sinensis from Malaria High-risk Area (말라리아 위험지역에서 채집된 말라리아 매개모기 Anopheles sinensis의 피레스로이드계 저항성 대립형질 분석)

  • Choi, Kwang Shik;Lee, Seung-Yeol;Hwang, Do-Un;Kim, Heung-Chul;Chang, Kyu-Sik;Jung, Hee-Young
    • The Korean Journal of Pesticide Science
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    • v.20 no.4
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    • pp.286-292
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    • 2016
  • Malaria is mainly transmitted by Anopheles sinensis which is dominant species in malaria high-risk area, northern part of Gyeonggi province in Korea. Pyrethroid insecticide is used for malaria vector, An. sinensis in Korea and the previous investigation consistently reported insecticide resistance from the vector. This study investigated insecticide susceptible and resistant alleles from An. sinensis and the status of malaria vector control in malaria high-risk area. For the study, An. sinensis collected from Paju, Gimpo and Ganghwa were sequenced for kdr detection. In Paju, there was no homozygous susceptibility and all of tested samples had homozygous or heterozygous resistance. There were 6.7% for susceptible homozygosity and 93.3% for resistant homozygosity or heterozygosity in Gimpo. Furthermore, the percentages of homozygous susceptibility and homozygous or heterozygous resistance in Ganghwa were 5.7% and 94.3% respectively. The results showed that the frequency of the insecticide resistance from An. sinensis in malaria high-risk area were increased much more than the previous investigation. Hence, this study suggests that malaria vector control programs should have to be prepared for the management of pyrethroid insecticide resistance.

Diamondback moth (Plutella xylostella L.) resistance to organophosphorus and carbamate insecticides in Kangwon alpine vegetable croplands (강원도 고랭지대 배추경작지 배추좀나방(Plutella xylostella L.)의 유기인계 및 카바메이트계 살충제에 대한 저항성 발달)

  • Cho, Jun-Mo;Kim, Kyoung-Ju;Kim, Song-Mun;Han, Dae-Sung;Hur, Jang-Hyun
    • The Korean Journal of Pesticide Science
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    • v.5 no.1
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    • pp.30-35
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    • 2001
  • Diamondback moth (Plutella xylostella L.) recently became a single worst insect which is not controlled effectively by organophosphorus and carbamate insecticides in Kangwon alpine croplands. The objective of this study was to determine if diamondback moth has developed a resistance to organophosphorus and carbamate insecticides. Resistance of diamondback moth, collected at Keichon, Jangpyong Taebaek, Chunchon, and Hongchon, was determined by the concentration required to kill fifty percent of population, $LC_{50}$. Their response of resistance varied to insecticides and locations: Taebaek populations were 35 and 70 times more resistant to chlorpyrifos and fenitrothion, respectively, than susceptible(S) population. Hongchon populations were 94 and 254 times more resistant to chlorpyrifos and fenitrothion, respectively, than S population. In addition, Chunchon populations were 37 and 19 times more resistant to profenofos and benfuracarb, respectively, than S population. However, the field populations did not differ in resistance to diazinon, phenthoate, flupyrazofos, carbofuran, and furathiocarb. This study show that field populations of diamondback moth found in Kangwon alpine vegetable croplands have developed a resistance and/or multiple resistance to some insecticides, implying that farmers are losing organophosphorus and carbamate insecticide options for selective control in vegetable crops.

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Comparison and Analysis of Insecticide Resistance of Two Spotted Spider Mite (Tetranychus urficae) among Apple Orchards (사과과수원 점박이응애의 약제 저항성 비교 분석)

  • Lee, Si-Woo;Kim, Kwang-Ho;Park, Chang-Gyoo;Park, Hong-Hyun;Lee, Kwan-Suk;Choi, Byeong-Ryeol;Lee, Sang-Guye
    • The Korean Journal of Pesticide Science
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    • v.14 no.3
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    • pp.266-271
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    • 2010
  • Six insecticides, monocrotophos (24%, Lq), milbemectin (1%, Ec), tebufenpyrad (10%, Ec), propargite (30%, Wp), dicofol (42%, Ec), and fenpropathrin (5%, Ec) were studied for their $LC_{50}s$ to local two-spotted spider mite (TSSM) strains collected at apple orchards in Chungju, Kunwi and Sobo in Korea. Monocrotophos and fenpropathrin were not effective due to resistance development, but milbemectin, tebufenpyrad, dicofol and propargite were effective to TSSM. $LC_{50}$ values to TSSM strains showed the same distribution pattern among apple orchards. However, TSSMs from different apple orchard were clustered into different groups.

Monitoring on Insecticide resistance of major insect pests in paddy field (주요 벼 해충에 대한 약제저항성 모니터링)

  • Lee, Si-Woo;Choi, Byeong-Ryeol;Park, Hyung-Man;Yoo, Jai-Ki
    • The Korean Journal of Pesticide Science
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    • v.9 no.4
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    • pp.365-373
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    • 2005
  • This study was carried out for looking into the status of susceptibility of pest insects to insecticides. Each insect Brown planthopper(BPH), Green leaf hopper(GLH), Smaller brown plant hopper(SBPH), Rice water weevil(RWW), were captured at various areas where the host crops were being cultivated and the susceptibility level of each pest insect was investigated. The susceptibility of each pest insect varied by insect species and areas where they were caught. BPHs kept higher level of susceptibility comparing to susceptible reference strain except to most of tested insecticides except imidacloprid (Resistance ratio was 68). The susceptibilities of GLH and SBPH to most of insecticides for their control did not developed markedly since 1976 except fipronil and imidacloprid which is widely used for WRR control. The insecticides used for control of WRR were very effective even at the concentration of one fourth of recommending concentration, but in 2000 suwon strain of WRR showed markedly reduction of susceptibility to carbofuran.

Strategy for Insecticide Resistance Management Approach to IPM

  • Motoyama, Naoki;Dauterman, W.C.
    • Korean journal of applied entomology
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    • v.31 no.3
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    • pp.314-327
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    • 1992
  • Insecticide resistance is a serious is a serious threat to IPM, resulting in various adverse effects not to mention the loss of yield in agriculture. One approach to counter the problem is the disruption of resistance mechanisms. This can be achieved by (1) compounds which show a negative correlation with resistance at the site of action, (2) specific metabolic inhibitors which serve as synergists, or (3) a certain combination of two insecticides producing a joint action. This approach, however, requires certain precautions for the side effects may cause an increase in toxicity to mammals. Owing to the recent advances in theoretical studies on resistance management employing computer simulation and mathematical models, a few principles to reduce the risk of development of resistance have been clarified. They are helpful in designing operational strategies with regard to, for instance, insecticide doses to be applied, mode of application, and choice and nature of the insecticide(s) to be used. For restoration of insecticide susceptibility of a resistant population, reintroduction of susceptible individuals to the resistant population is feasible when certain conditions are met. Natural enemies which developed resistance to insecticides can be an important component of IPM as has been shown in the pest management in apple orchards. After all, the implementation of a successful resistance management program depends upon cooperation between different sigments of the agricutural community. Although resistance is a preadaptive phenomenon, in some cases spontaneous loss of resistance does occur without contamination by susceptible individuals. The instability of resistance in these insects implies the possible existence of a switch machanism controlling the expression of resistance gene(s). Elucidation of such a mechanism may eventually provide us with a new technical approach with which we can combat the problem of insecticide resistance.

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Monitoring for the Resistance of Botrytis cinerea Causing Gray Mold Against Mepanipyrim (Mepanipyrim에 대한 잿빛곰팡이병균의 저항성 검정)

  • Kim, Ah Hyeong;Kim, Seon Bo;Han, Kee Don;Kim, Heung Tae
    • The Korean Journal of Pesticide Science
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    • v.19 no.3
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    • pp.329-334
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    • 2015
  • With 86 isolates of Botrytis cinerea obtained from diseased fruits of tomato, strawberry and cucumber, it was conducted to detect the fungicide resistance of B. cinerea against mepanipyrim through an agar dilution method. FGA medium was used for monitoring the resistance. Among 86 isolates of B. cinerea, resistant isolates were composed by 23.3%, which was different according to regions isolating the pathogen. In accordance with isolation region, the isolation frequency of resistant isolates was as follows; that of Gyeonggi, Gyeongnam/Pusan and chungnam was 28.6%, 33.3% and 12.8%, respectively. The resistant isolates of B. cinerea showed the cross resistance between mepanipyrim and pyrimethanil. Also the pathogenicity of B. cinerea resistant to mepanipyrim was high as like that of sensitive isolates. Because of them, it should be necessary to manage the spraying system of mepanipyrim and the monitoring for the fungicide resistance.

Detection for the Resistance of Fusarium spp. Isolated from Rice Seeds to Prochloraz and Cross-resistance to Other Fungicides Inhibiting Sterol Biosynthesis (벼 종자에서 분리한 Fusarium속 균주들의 prochloraz에 대한 저항성 검정 및 교차 저항성 조사)

  • Shin, Myeong-Uk;Kang, Hyo-Jung;Lee, Yong-Hwan;Kim, Heung-Tae
    • The Korean Journal of Pesticide Science
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    • v.12 no.3
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    • pp.277-282
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    • 2008
  • To assess the resistance to prochloraz, $EC_{50}$ values of Fusarium isolates obtained from rice seed were investigated through the agar dilution method. $EC_{50}$ value of 36 isolates of Fusarium spp. to prochloraz ranged from 0.020 to $1.78{\mu}g\;mL^{-1}$ with an average of $0.25{\mu}g\;mL^{-1}$. According to the species of Fusarium, the average $EC_{50}$ value was fluctuated; $0.091{\mu}g\;mL^{-1}$ for F. moniliformis, $0.11{\mu}g\;mL^{-1}$ for F. proliferatum and $0.31{\mu}g\;mL^{-1}$ for F. fujikuroi. The resistant baseline was decided at $0.5{\mu}g\;mL^{-1}$ to determine if the isolate was resistant to prochloraz or not. Based on the resistant baseline, the ratio of resistant isolates was 14%. There was no correlation between the resistance to prochloraz and the pathogenicity of Fusarium spp. on rice seedlings. The resistant isolates of F. fujikuroi did not show the cross-resistance to other sterol biosynthesis inhibiting fungicides, triflumizole, hexaconazole, difenoconazole and tebuconazole.

Cross Resistance and Point Mutation of the Mitochondrial Cytochrome b of Bifenazate Resistant Two-spotted Spider Mite, Tetranychus urticae (Bifenazate 저항성 점박이응애에 대한 교차저항성과 미토콘드리아 cytochrome b의 점돌연변이)

  • Lee, Kyo-Ri;Koo, Hyun-Na;Yoon, Chang-Mann;Kim, Gil-Hah
    • The Korean Journal of Pesticide Science
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    • v.14 no.3
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    • pp.247-254
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    • 2010
  • Two-spotted spider mite, Tetranychus urticae was collected from the rose greenhouse in Chilgok, Gyeongbuk Province in December 2000. This population has been selected for ten years with bifenazate (over 450 times), and increased 855.9 fold in resistance as compared with susceptible strain (S). Cross resistance of bifenazate resistant (BR) strain to eight miticides was investigated. The BR strain exhibited high and low cross resistance to acequinocyl (614.0 fold) and to chlorfenapyr (9.1 fold), respectively. Against fenazaquin (0.3 fold) and fenpyroximate (0.1 fold), however, showed the strain negatively correlated cross resistance. Each strain collected in Choeng-ju (CJ), Kang-jin (KJ), and Chung-ju (CUJ) showed 5.5-, 964.5-, and 21.8-fold resistance to bifenazate, respectively. The detoxifying enzymes of the BR strain showed 1.6-fold activity in cytochrome $P_{450}$-dependent monooxygenase ($P_{450}$) as compared with susceptible one. By comparing the mitochondrial cytochrome b (cytb) sequence, G126S point mutation was detected in the BR and KJ strains.