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http://dx.doi.org/10.7585/kjps.2016.20.4.286

Analysis of Pyrethroid Resistance Allele in Malaria Vector Anopheles sinensis from Malaria High-risk Area  

Choi, Kwang Shik (School of Life Science, College of Natural Sciences, Kyungpook National University)
Lee, Seung-Yeol (School of Applied Biosciences, College of Agriculture and Life Sciences, Kyungpook National University)
Hwang, Do-Un (School of Life Science, College of Natural Sciences, Kyungpook National University)
Kim, Heung-Chul (5th Medical Detachment, 168th Multifunctional Medical Battalion, 65th Medical Brigade)
Chang, Kyu-Sik (Division of Medical Entomology, Korea Center for Disease Control and Prevention)
Jung, Hee-Young (School of Applied Biosciences, College of Agriculture and Life Sciences, Kyungpook National University)
Publication Information
The Korean Journal of Pesticide Science / v.20, no.4, 2016 , pp. 286-292 More about this Journal
Abstract
Malaria is mainly transmitted by Anopheles sinensis which is dominant species in malaria high-risk area, northern part of Gyeonggi province in Korea. Pyrethroid insecticide is used for malaria vector, An. sinensis in Korea and the previous investigation consistently reported insecticide resistance from the vector. This study investigated insecticide susceptible and resistant alleles from An. sinensis and the status of malaria vector control in malaria high-risk area. For the study, An. sinensis collected from Paju, Gimpo and Ganghwa were sequenced for kdr detection. In Paju, there was no homozygous susceptibility and all of tested samples had homozygous or heterozygous resistance. There were 6.7% for susceptible homozygosity and 93.3% for resistant homozygosity or heterozygosity in Gimpo. Furthermore, the percentages of homozygous susceptibility and homozygous or heterozygous resistance in Ganghwa were 5.7% and 94.3% respectively. The results showed that the frequency of the insecticide resistance from An. sinensis in malaria high-risk area were increased much more than the previous investigation. Hence, this study suggests that malaria vector control programs should have to be prepared for the management of pyrethroid insecticide resistance.
Keywords
Anopheles sinensis; insecticide resistance; knockdown resistance; pyrethroid insecticide;
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1 Mnzava, A. P., T. B. Knox, E. A. Temu, A. Trett, C. Fornadel, J. Hemingway and M. Renshaw (2015) Implementation of the global plan for insecticide resistance management in malaria vectors: progress, challenges and the way forward. Malar. J. 14:173.   DOI
2 Park, J. W., T. A. Klein, H. C. Lee, L. A. Pacha, S. H. Ryu, J. S. Yeom, S. H. Moon, T. S. Kim, J. Y. Chai, M. D. Oh and K. W. Choe (2003) Vivax malaria: a continuing health threat to the Republic of Korea. Am. J. Trop. Med. Hyg. 69:159-167.
3 Ree, H. I. (2005) Studies on Anopheles sinensis, the vector species of vivax malaria in Korea. Korean J. Parasitol. 43:75-92.   DOI
4 Rueda, L. M., C. Li, H. C. Kim, T. A. Klein, D. H. Foley and R. C. Wilkerson (2010) Anopheles belenrae, a potential vector of Plasmodium vivax in the Republic of Korea. J. Am. Mosq. Control Assoc. 26:430-432.   DOI
5 Wang, D., Z. Xia, S. Zhou, X. Zhou, R. Wang and Q. Zhang (2013) A potential threat to malaria elimination: extensive deltamethrin and DDT resistance to Anopheles sinensis from the malaria-endemic areas in China. Malar. J. 12:164.   DOI
6 World Health Organization (1981) Synopsis of the world malaria situation in 1979. The Weekly Epidemiological Record 56:145-149.
7 World Health Organization (1998) Test procedures for insecticide resistance monitoring in malaria vectors, bio-efficacy and persistence of insecticides on treated surfaces. WHO/CDS/CPC/MAL/98.12 Geneva, Switzerland.
8 World Health Organization (2012) Global Plan for Insecticide Resistance Management in Malaria Vectors. Geneva, Switzerland: World Health Organization.
9 World Health Organization (2014) Global Malaria Programme, World malaria report. Geneva: World Health Organization.
10 World Health Organization (2015) Global Malaria Programme, World malaria report. Geneva: World Health Organization.
11 Hoti, S. L., V. Vasuki, P. Jambulingam and S. S. Sahu (2006) kdr allele-based PCR assay for detection of resistance to DDT in Anopheles culicifacies sensu lato Giles population from Malkangiri District, Orissa, India. Curr. Sci. 91:658-661.
12 Chang, K. S., J. S. Jung, C. Park, D. K. Lee and E. Shin (2009) Insecticide susceptibility and resistance of larvae of the Anopheles sinensis group (Diptera: Culicidae) from Paju, Republic of Korea. Entomol. Res. 39:196-200.   DOI
13 Chang, K.-S., D.-H. Yoo, E.-H. Shin, W.-G. Lee, J. Y. Roh and M. Y. Park (2013) Susceptibility and resistance of field populations of Anopheles sinensis (Diptera: Culicidae) collected from Paju to 13 insecticides. Osong Public Health Res. Perspect. 4:76-80.   DOI
14 Chang, K. S., D.-H. Yoo, Y. R. Ju, W. G. Lee, J. Y. Roh, H.-C. Kim, T. A. Klein and E.-H. Shin (2016) Distribution of malaria vectors and incidence of vivax malaria at Korean army installations near the demilitarized zone, Republic of Korea. Malar. J. 15:259.   DOI
15 Diabate, A., C. Brengues, T. Baldet, K. R. Dabir, J. M. Hougard, M. Akogbeto, P. Kengne, F. Simard, P. Guillet, J. Hemingway and F. Chandre (2004) The spread of the Leu-Phe kdr mutation through Anopheles gambiae complex in Burkina Faso: genetic introgression and de novo phenomena. Trop. Med. Int. Health 9:1267-1273.   DOI
16 Gayathri, V. and P. B. Murthy (2006) Reduced susceptibility to deltamethrin and kdr mutation in Anopheles stephensi Liston, a malaria vector in India. J. Am. Mosq. Control Assoc. 22:678-688.   DOI
17 Hall, T. A. (1999) Bioedit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp. Ser. 41:95-98.
18 Hemingway, J., N. J. Hawkes, L. McCarroll and H. Ranson (2004) The molecular basis of insecticide resistance in mosquitoes. Insect Biochem. Mol. Biol. 34:653-665.   DOI
19 Hunt, R. H., G. Fuseini, S. Knowles, J. Stiles-Ocran, R. Verster, M. L. Kaiser, K. S. Choi, L. L. Koekemoer and M. Coetzee (2011) Insecticide resistance in malaria vector mosquitoes at four localities in Ghana, West Africa. Parasite. Vector. 4:107.   DOI
20 Joshi, D., W. Choochote, M. H. Park, J. Y. Kim, T. S. Kim, W. Suwonkerd and G. S. Min (2009) The susceptibility of Anopheles lesteri to infection with Korean strain of Plasmodium vivax. Malar. J. 8:42.   DOI
21 Kim, H., J. H. Baek, W. J. Lee and S. H. Lee (2007) Frequency detection of pyrethroid resistance allele in Anopheles sinensis populations by real-time PCR amplification of specific allele (rtPASA). Pestic. Biochem. Phys. 87:54-61.   DOI
22 Joshi, D., M. H. Park, A. Saeung, W. Choochote and G. S. Min (2010) Multiplex assay to identify Korean vectors of malaria. Mol. Ecol. Resour. 10:748-750.   DOI
23 Joshi, D., J. Y. Kim, W. Choochote, M. H. Park and G. S. Min (2011) Preliminary vivax malaria vector competence for three members of the Anopheles Hyrcanus group in the Republic of Korea. J. Am. Mosq. Control Assoc. 27:312-314.   DOI
24 Kang, S., J. Jung, S. Lee, H. Hwang and W. Kim (2012) The polymorphism and the geographical distribution of the knockdown resistance (kdr) of Anopheles sinensis in the Republic of Korea. Malar. J. 11:151.   DOI
25 Korea Centers for Disease Control and Prevention (KCDC) (2012) A Guideline of Malaria Management, Republic of Korea. Korea Center for Disease Control and Prevention 49-70.
26 Korea Center for Disease Control and Prevention (KCDC) (2015) A Guideline of Malaria Management, Republic of Korea. Korea Center for Disease Control and Prevention 49-72.
27 Lee, W. J., T. A. Klein, H. C. Kim, Y. M. Choi, S. H. Yoon, K. S. Chang, S. T. Chong, I. Y. Lee, J. W. Jones, J. S. Jacobs, J. Sattabongkot and J. S. Park (2007) Anopheles kleini, Anopheles pullus, and Anopheles sinensis: potential vectors of Plasmodium vivax in the Republic of Korea. J. Med. Entomol. 44: 1086-1090.
28 Li, C., J. S. Lee, J. L. Groebner, H. C. Kim, T. A. Klein, M. L. O'guinn and R. C. Wilkerson (2005) A newly recognized species in the Anopheles Hyrcanus Group and molecular identification of related species from the Republic of South Korea (Diptera: Culicidae). Zootaxa 939:1-8.   DOI