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Cross Resistance and Point Mutation of the Mitochondrial Cytochrome b of Bifenazate Resistant Two-spotted Spider Mite, Tetranychus urticae  

Lee, Kyo-Ri (Department of Plant Medicine, College of Agriculture, Life, and Environment Sciences, Chungbuk National University)
Koo, Hyun-Na (Department of Plant Medicine, College of Agriculture, Life, and Environment Sciences, Chungbuk National University)
Yoon, Chang-Mann (Department of Plant Medicine, College of Agriculture, Life, and Environment Sciences, Chungbuk National University)
Kim, Gil-Hah (Department of Plant Medicine, College of Agriculture, Life, and Environment Sciences, Chungbuk National University)
Publication Information
The Korean Journal of Pesticide Science / v.14, no.3, 2010 , pp. 247-254 More about this Journal
Abstract
Two-spotted spider mite, Tetranychus urticae was collected from the rose greenhouse in Chilgok, Gyeongbuk Province in December 2000. This population has been selected for ten years with bifenazate (over 450 times), and increased 855.9 fold in resistance as compared with susceptible strain (S). Cross resistance of bifenazate resistant (BR) strain to eight miticides was investigated. The BR strain exhibited high and low cross resistance to acequinocyl (614.0 fold) and to chlorfenapyr (9.1 fold), respectively. Against fenazaquin (0.3 fold) and fenpyroximate (0.1 fold), however, showed the strain negatively correlated cross resistance. Each strain collected in Choeng-ju (CJ), Kang-jin (KJ), and Chung-ju (CUJ) showed 5.5-, 964.5-, and 21.8-fold resistance to bifenazate, respectively. The detoxifying enzymes of the BR strain showed 1.6-fold activity in cytochrome $P_{450}$-dependent monooxygenase ($P_{450}$) as compared with susceptible one. By comparing the mitochondrial cytochrome b (cytb) sequence, G126S point mutation was detected in the BR and KJ strains.
Keywords
Tetranychus urticae; Bifenazate; Cross resistance; Point mutation; Cytochrome b;
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