• Parasites, Hosts and Diseases
• /
• 제25권1호
• /
• pp.45-50
• /
• 1987

감작복강삼출세포가 BALB/c마우스에서 간흡충에 대한 면역을 이입 할 수 있는지 규명하기 위해 10마리의 donor마우스를 5마리씩 2군으로 나누어 한 군에는 20개의 간흡충 피낭유충을 강구감염시켰고 다른 군에는 20마리의 본 탈낭유충을 간강내 주입한 30일 후에 도살하여 복강삼출세포를 얻었다. 제1차 감작을 하기위해 20마리의 recipient마우스를 4마리씩 4군으로 나누어 제1군은 간흡충 피낭유충을 가구감염시킨 donor마우스의 복강삼출세포를 recipient마우스의 복강내 주입하였고, 제2군은 본 탈낭유충을 간강내 주입한 donor마우스의 복강삼출세포를 recipient마우스의 복강내 주입하였으며, 제3군은 20개의 본 피낭유충을 경구감소시켰다. 제4군은 비처치대조군으로 하였다. 제1차 감작한 4일후에 전 recipient마우스에 20개의 본 피낭유충을 challenge강구감소시켜 30일후에 모두 도살하여 대조군의 대편 g당 충란수, worm burden 및 비장당에 의한 면역이입의 여부를 판정하였다. Donor마우스의 복강삼출세포를 recipient마우스의 복강내 주입하였을 때 제1군과 제2군마우스에서는 제4군마우스에 비해 대편 g당 충란수의 현저한 감소를 나타내었으나 제3군에서는 그 충란수가 감소되지 않았다. Worm burden은 제2군 마우스에서만 제4대조군에 비해 유의적 감소를 나타내었으며 plaque형성세포는 제1군보다 제2군에서 많이 검출되었고 특히 간흡충 피낭유충으로 감작한 복강삼출세포를 복강내 주입받은 BALB/c마우스에서는 면역에 이입됨을 알았다.

• 한국응용약물학회:학술대회논문집
• /
• 한국응용약물학회 1996년도 춘계학술대회
• /
• pp.177-177
• /
• 1996

Taurine Chloramine (Tau-Cl) inhibits production of nitric oxide(NO) and tumor necrosis factor-alpha (TNF-${\alpha}$) in activated peritoneal macrophages, similar In that previously reported for activated RAW 264.7 cells. In addition, the effect of Tau-Cl and taurine on superoxide anion (O$\_$2/$\^$-/) Production in murine peritoneal exudate polymorphonuclear leukocytes (PMN) was examined. Tau-Cl inhibited O$\_$2/$\^$-/ production in a manner that was dose-dependent and reversible, Taurine also inhibited O$\_$2/$\^$-/ production by stimulated PMN, but at higher concentrations and to a lesser extent than Tau-Cl. The effects of taurine on O$\_$2/$\^$-/ production was attributed to the in vitro formation of Tau-Cl catalyzed by PMN associated halide-dependent myeloperoxidase. In contrast, production of NO and TNF-${\alpha}$ by activated peritoneal exudate macrophages was inhibited by Tau-Cl while taurine was without effect. These data lend support to the notion that Tau-Cl may participate ill the inflammatory responses by modulating production of inflammatory mediators.

• 약학회지
• /
• 제31권6호
• /
• pp.347-354
• /
• 1987

The effect of vitamin A on the immune response in ICR mice was studied. The effects were evaluated by immuno organ weight, peripheral circulating white blood cells, HA and HY titer, peritoneal exudate cells, RFC, Arthus reaction and DTH in mice. The spleen of mice was significantly hypertrophied by deficiency or over doses of vitamin A as compared with control group (50IU/kg). Arthus reaction, RFC and peritoneal exudate cells were sharply decreased according to the increase of vitamin A doses. The number of white blood cell was decreased according to the increase of vitamin A doses, but in the case of vitamin A 50,000 IU/kg treated group, it was significantly increased. These results suggest that deficiency or over dose of vitamin A decrease humoral and cellular immune response.

• KSBB Journal
• /
• 제7권1호
• /
• pp.39-44
• /
• 1992

다당류의 ICR 마우스에서 백혈구수와 복강세포수가 현저히 증가하였고, 면역 관련 장기의 무게도 용량에 따라 증가하는 용량 의존성 반응을 보였고, maerophage의 식작용에 미치는 영향은 phagocytic index와 corrected phagocytic index에서 거의 차이가 없었다. 다당류가 마우스의 혈액 중에 효소 활성 및 일반 생화학적 성분에 미치는 영향을 조사해 본 결과 S-GOT, S-GPT, alkaline phosphatase, total protein, albumin, globulin, Cholesterol, triglyceride, blood urea nitrogen 및 glucose는 대조군에 비하여 비슷한 수준을 나타내었다.

• 약학회지
• /
• 제35권3호
• /
• pp.165-173
• /
• 1991

Effects of eight flavonoids and their related compounds on leukocytes migration, superoxide anion production and lipid peroxidation in the phagocytosis of latex beads or E. coli by guinea pig peritoneal exudate cells were studied in vitro. It shows that most of flavonoids generally inhibited the leukocytes migration and production of superoxide anion and malonedialdehydes. Their inhibitory activities in the phagocytosis of latex beads had more active than that of E. coli. Quercetin has the most inhibitory activity in leukocytes migration and production of superoxide anion and lipid peroxides at the concentration of 1, 2 and 10 $\mu{M}$. Catechin and rutin at the concentration of 2 and 10 $\mu{M}$ inhibited significantly the production of superoxide anion and lipid peroxides. Flavone, catechin, naringin and rutin at the concentration of 2 and 10 $\mu{M}$ inhibited significantly the leukocytes migration.

• 생약학회지
• /
• 제16권4호
• /
• pp.181-190
• /
• 1985

To determine contents of inorganic elements of Ganoderma lucidum, the horn-shaped carpophores and the pileus of Ganoderma lucidum were incinerated and analyzed by inductively coupled plasma atomic emission spectrophotometry. The ash contents of the pileus and the horn-shaped carpophore were 1.48% and 1.40%, respectively. The pileus contained calcium, magnesium, sodium, manganese, iron, zinc and germanium in that order. The horn-shaped carpophore contained magnesium, calcium, zinc, manganese, iron, copper and germanium in that order. To examine the protein-bound polysaccharide from Ganoderma lucidum for immunopotentiating activity, its fruit bodies were extracted with hot water. Purification of the extract was carried out by acetone precipitation and dialysis. The fraction obtained during the purification procedure consisted of a polysaccharide moiety (51%) and a protein moiety (5%). When the compound was administered intraperitoneally to the mice at a dose of 50mg/kg, it enhanced the accumulation of the peritoneal exudate cells, macrophage and polymorphonuclear leucocytes, thereby indicating immunopotentiation.

• Parasites, Hosts and Diseases
• /
• 제33권3호
• /
• pp.195-200
• /
• 1995

톡소포자충에 감염된 마우스의 복수액이 Con A로 유도한 정상 마우스 복수 림프구의 아세포화를 억제하는 효과를 관찰하였다. 마우스 감염은 톡소포자충의 RH주를 사용}하였다. ConA로 유도한 아세포화 정도는 정상 마우스의 복수 림프구를 얻어 96-well 배양기에 될주하고 Con A를 $10{\;}\mu\textrm{g}/ml$로 5일간 처리하면서, 4일째에 $^3H-thymidine$을 well 당 $1{\;}{\mu}Ci$씩 첨가하여 DNA 표지량으로 측정하였다. 아세포화 억제효과는 Con A와 감염 복수액을 동시 처리한 군의 아세포화를 Con A만 처리한 군에서의 아세포화에 대한 비율로 나타내었다. Con A에 의한 아세포화는 7.3배였다. 정상 마우스의 복수액에 의한 아세포화 억제효과는 $16.4{\;}{\pm}{\;}8.3%$이나, 감염 4일째 마우스의 복수액은 아세포화를 $74.0{\;}{\pm}{\;}11.9%$ 억제하였다. 생존일 4-5일의 감염 마우스에서 복수액을 일자별로 채취하여 처리하면 감염 1일에 $16.9{\;}{\pm}{\;}7.9%$, 2일에 $23.4{\;}{\pm}{\;}8.3%$, 3일에 $41.4{\;}{\pm}{\;}7.1%$ 및 4일에 $74.0{\;}{\pm}{\;}11.9%$로 높은 억제효과가 발현되었다. 감염후 4일에 얻은 복수액을 희석하여 처리하였을 때 아세포화 억제 효과는 농도에 따라 변화하였다. 감염복수액을 $95^{\circ}C$에서 10분간 열처리하면 억제 효과가 소멸되었으며, 10% TCA로 침전시킨 후 상층액으로 처리하여도 억제효과가 소멸하였다. 따라서 톡소포자충 감염 마우스 복수액에 복수 림프구의 아세포화를 억제하는 물질이 존재하며 그 생체활성물질은 열에 약한 단백질이라고 판단하였다.

• Parasites, Hosts and Diseases
• /
• 제40권3호
• /
• pp.119-129
• /
• 2002

Although there are many reports on the splenic (systemic) T cell response after Toxoptasma gondii infection, little information is available regarding the local T cell responses of peritoneal exudate cells (PEC) and gut intraepithelial Iymphocytes (IEL) following peroral infection with bradyzoites. Mice were infected with 40 cysts of the 76K strain of T. gondii, and then sacrificed at days 0, 1, 4, 7 and 10 postinfection (PI). The cellular composition and T cell responses of PEC and IEL were analyzed. The total number of PEC and IEL per mouse increased after infection, but the ratio of increase was higher in IEL. Lymphocytes were the major component of both PEC and IEL. The relative percentages of PEC macrophages and neutrophils/eosinophils increased signiflcantly at day 1 and 4 PI, whereas those of IEL did not change significantly. The percentage of PEC NK1.1 and ${\gamma\delta}T$ cells peaked at day 4 PI (p < 0.0001), and CD4 and $CD8{\alpha}T$ cells increased continuously after infection. The percentages of IEL $CD8{\alpha}$ and ${\gamma\delta}T$ cells decreased slightly at first, and then increased. CD4 and NK1.1 T cells of IEL did not change significantly after infection. $IFN-{\gamma}-producing$ PEC NK1.1 T cells increased significantly from day 1 PI, but the other T cell subsets produced $IFN-{\gamma}$ abundantly thereafter. The proportion of IEL $IFN-{\gamma}-producing$ $CD8{\alpha}$ and ${\gamma\delta}T$ cells increased significantly after infection, while IEL NK1.1 T cells had similar $IFN-{\gamma}$ production patterns. Taken together, CD4 T cells were the major phenotype and the important $IFN-{\gamma}$ producing T cell subsets in PEC after oral infection with T. gondii whereas $CD8{\alpha}T$ cells had these roles in IEL. These results suggest that PEC and IEL comprise different cell differentials and T cell responses, and according to infection route these factors may contribute to the different cellular immune responses.

• 약학회지
• /
• 제31권2호
• /
• pp.70-81
• /
• 1987

To elucidate action mechanism of lyophyllan A, an antitumor polysaccharide of Lyophyllum decastes, its immunological activities were examined. Lyophyllan A increased significantly the weights of spleen and liver of mice. Lyophyllan A also restored the decreased thymic weight in tumor-bearing mice. It did not show any direct cytotoxicity against tumor cells, but showed immunopotentiating activities by increasing the number of the plaques in hemolytic plaque assays. Lyophyllan A increased the number of peritoneal exudate cells (PEC) and inhibited the growth of sarcoma 180 mixed with PEC. Moreover the macrophages from lyophyllan A-treated mice exhibited a strong cytotoxic activity towards L5178Y target cells.

• Archives of Pharmacal Research
• /
• 제14권1호
• /
• pp.68-72
• /
• 1991

The polysaccharide fraction from Taraxii Herba showed potent immunopotentiating activities with antitumor activities. The fraction having small amount of protein inhibited the growth of solid tumor and increased peritoneal exudate cells and immunoorgan weights in normal mice, and also increased hypersensitivities in tumor bearing mice.