• KOREAN JOURNAL OF CROP SCIENCE
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• v.49 no.3
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• pp.222-226
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• 2004

This study was conducted to measure the major agronomic characteristics of 85 local varieties of perilla at Chungbuk Agricultural Research and Extension Services in 2001. Perilla accessions examined were classified into three maturity groups, i.e, early, medium, and late maturity group of 19 (23％),57 (67％), and 9 (10％) accessions, respectively. The early and late maturity groups mainly consisted of collections from middle-northern area and southern area, respectively, while the medium group consisted of accessions from all over the country. Average 1,000-seed weight (TSW) was 2.7 g. TSW of the collection from Hamyang was the greatest a 3.9 g, while the TSW of Pyungchang collection was the smallest as 1.7 g, and most of collections produced medium and small seeds. Perilla accessions with greate 1,000-seed weight seemed to be belonged to the late maturity group. Seed coat colors of perilla accessions were dark brown (30％), brown (55％) and gray brown (6％), respectively, Among seed coat colors, brown color consisted of 91％. Stem height, the number of nodes, branches, flower clusters, and capsules per flower cluster, and the length of flower cluster were positively correlated occ, while these characteristics were negatively correlated with the number of capsules per flower cluster and 1,000-seed weight. These results allowed us to select 5 perilla collections, containing 1 collection with gray white seed color, and 4 collections over 2.5g 1000 seed weight, as parental lines in the breeding program.

• Korean Journal of Breeding Science
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• v.50 no.4
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• pp.463-471
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• 2018

Perilla is an oilseed crop cultivated in Korea since ancient times. Due to the high ${\alpha}-linolenic$ acid content in perilla, perilla seed oil can easily become rancid. ${\alpha}-Linolenic$ acid is synthesized by two enzymes, endoplasmic reticulum-localized ${\Delta}15$ desaturase (FAD3) and chloroplast-localized ${\Delta}15$ desaturase (FAD7) in vivo. In order to lower the ${\alpha}-linolenic$ acid content of the seed oil without disturbing plant growth, we tried to suppress the expression of only the FAD3 gene using RNA interference, whilst maintaining the expression of the FAD7 gene. Seventeen transgenic plants with herbicide ($Basta^{TM}$) resistance were obtained by Agrobacterium-mediated transformation using hypocotyls of perilla plants. The transgenic plants were firstly confirmed by treatment with 0.3% (v/v) $Basta^{TM}$ herbicide, and the expression of FAD3 was measured by Northern blot analysis. The ${\alpha}-linolenic$ acid content was 10-20%, 30-40%, and 60% in two, seven, and three of the twelve $T_1$ transgenic perilla plants which had enough seeds to be analyzed for fatty acid composition, respectively. Analysis of the fatty acid composition of $T_2$ progeny seeds from $T_1$ plants with the lowest ${\alpha}-linolenic$ acid content showed that the homozygous lines had 6-10% ${\alpha}-linolenic$ acid content and the heterozygous lines had 20-26% ${\alpha}-linolenic$ acid content. It is expected that the reduction in ${\alpha}-linolenic$ acid content in perilla seed oil will prevent rancidity and can be utilized for the production of high-value functional ingredients such as high ${\gamma}-linolenic$ acid.

• Korean Journal of Plant Resources
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• v.17 no.2
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• pp.213-218
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• 2004

This study was conducted to examine the variations in 42 local perilla collected in Kangwon province of Korea and to provide basic informations which can be utilize in perilla breeding programs. The results obtained were summarized as the follows. Maturing period was widely distributed from 109 to 140 days. The maturing periods of most collections were 116 to 130 days(95.2％). and that 4.8％ of collections shown maturing period of below 115 days and above 131 days. Three varieties 109 day of maturing period were selected among local perilla collected from Yanggu gun. Stem length was ranged from 80 to 140 cm. The stem length of 111 to 130 cm, below 111 cm, and above 131 cm were 54.8％, 38.6％, and 16.7％, respectively. The number of cluster per plant was distributed 23 to 120. The distribution of cluster numbers were 40 to 70, under 40, and above 100 was 57.2％, 16.7％, and 11.9％, respectively. The number of seed capsules was distributed 20 to 40 and the collections ranged from at of 30 to 35 was 38.1 ％. Seed cluster length was distributed from 5.1 to 9.0 cm and that ranged from 6.1 to 8.0 cm was 64.3％, 1,000 grain weight ranged from 1.7 to 3.8 g. The distribution of 1,000 grain weight is 2.4 to 2.6 g, 3.0 to 3.2 g, and below 2.9 g was 35.7％, 19.1％, respectively. The 1,000 grain most weight 3.8 g was selected among local perilla collected in Hwacheon gun.

• Korean Journal of Plant Resources
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• v.33 no.1
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• pp.40-49
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• 2020

The objectives of this study were to evaluate total phenolic content (TPC) and individual phenolic compounds in leaves of perilla genetic resources, assess whether they could be used as distinguishing factor among germplasms, and evaluate their relationship with some quantitative and qualitative morphological characters. TPC and individual phenolic compounds were determined using Folin-Ciocalteu method and UPLC-PDA system, respectively. Wide variations in TPC (7.99 to 133.70 mgGAE/g DE), rosmarinic acid (ND to 21.05 mg/g DE), caffeic acid (ND to 1.17 mg/g DE), apigenin-7-O-diglucuronide (ND to 2.21 mg luteolin equivalent (mgLUE)/g DE), scutellarein-7-O-glucuronide (ND to 5.25 mg LUE/g DE), and apigenin-7-O-glucuronide (ND to 2.81 mg LUE/g DE) were observed. Intensities of green pigment at abaxial and adaxial leaf surfaces were positively correlated with phenolic compounds whereas leaf length and width had negative correlation. Purple pigmented accessions were shorter in leaf length and width but exhibited higher amount of phenolic compounds compared to green pigmented accessions in most cases. Leaf shape was not related with content of phenolic compounds, color of leaves, and length/width of leaves. TPC and individual phenolic compounds along with morphological characters could be useful distinguishing factors for perilla genetic resources.

• Food Science and Biotechnology
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• v.18 no.3
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• pp.793-798
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• 2009

The objectives of this present study were to compare the contents and determine optimum extraction conditions for the rosmarinic acid (RA) and caffeic acid (CA) from leaves of Korean Perilla frutescens varieties. RA and CA from leaves of cv. Bora, a breeding line of P. frutescens were isolated and elucidated using various spectroscopic data. On the basis of 2 phenolic acids, optimum extraction conditions were obtained by employing 50% EtOH for 60 min at $25^{\circ}C$. We reported for the first time on the contents of RA and CA from leaves of 32 Korean varieties. Among them, leaves of P. frutescens Brit. var. acuta Kudo I exhibited the highest RA content ($8.53{\pm}0.57$ mg/g) and CA content ($2.33{\pm}0.11$ mg/g) showed the highest in the P. frutescens Brit. var. viridis Makino. Interestingly, average RA content ($2.66{\pm}0.17$ mg/g) showed a markedly higher than that of CA ($1.98{\pm}0.16$ mg/g) in Korean varieties. These results suggest that concentrations of the RA and CA in P. frutescens leaves could be a key factor in the selection process of a high quality species.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.27 no.4
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• pp.470-479
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• 1982

Researches on industrial crops in Korea before 1962 were concentrated on fiber crops such as cotton, kenaf, hemp, ramie and flax. Then research works on oil crops, sugar crops and other high income crops were followed. However, no land is shared for the production of kenaf, flax, sugar beet, sweet sorghum and sunflower at present in Korea, while the cultivation of cotton, hemp, ramie and mat rush is decreasing continuously to the marginal point. At present researches are emphasized on oil crops such as seasame, peanut and perilla and high income medicinal herbs of which cultivating acreages are increasing. Numerous varieties were released as a result of active breeding works on industrial crops since 1962, i.e. 3 sesame varieties including "Suweon 21", 3 peanut varieties including "Seoduntangkong", and 6 rape varieties including "Yudal" in oil crops, one cotton variety "Mokpo 7", one hemp variety "MS4-1", and one kenaf variety "Suweon 2" in fiber crops, and two stevia varieties "Suweon 2" and "Suweon II" in sugar crops. Quality improvement of rape seeds and development of hybrid rapes utilizing male sterile lines are the most significant results of breeding works, while the establishment of vinyl mulching cultivation of sesame and peanut are the most successful results in agronomic researches during the last 20 years.re the most successful results in agronomic researches during the last 20 years.

• Korean Journal of Breeding Science
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• v.43 no.4
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• pp.288-296
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• 2011

To clarify the seed germination characteristics among cultivated Perilla crop and their weedy types in Korea, we studied the germination percent and germination energy of 162 accessions (102 cultivated var. frutescens, 41 weedy var. frutescens, and 19 weedy var. crispa) in both conditions of $4^{\circ}C$ low temperature treatment and non-cold treatment. In our study, most accessions of cultivated var. frutescens showed more than 50% in both germination percent and germination energy in both cold and non-cold treatment conditions. Whereas, most accessions of weedy var. frutescens and weedy var. crispa showed lower than 50% in both germination percent and germination energy in both cold and non-cold treatment. In addition, most accessions of Perilla crop and their weedy types showed much higher germination percent and germination energy in $4^{\circ}C$ low temperature treatment condition compared to the seeds under non-cold treatment condition. The information provided in this research may help for our understanding the variation of seed germination characteristics among accessions of cultivated Perilla crop and their weedy types in Korea.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.66 no.3
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• pp.248-255
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• 2021

This study was conducted to obtain basal information for the development of perilla cultivars with improved quality. The F₇ population of recombinant inbred lines (RILs) from a cross between the parents of Daesil for seeds and Ipdeulkkae1 for vegetables was used as the material. We evaluated several agricultural characteristics and seed quality. Variations were observed in most of the measurements; for example, stem length (ranging from 66.0 to 150.0 cm), number of branches (from 5 to 23), flower cluster length (ranging from 5.1 to 10.5 cm), number of flower clusters (from 17 to 131), a-linolenic acid content (from 54.2 to 64.1%), and functional compound content (rosmarinic acid 869.5~3,508.1 ㎍/g; luteolin 47.4~864.3 ㎍/g; apigenin 57.1~296.7 ㎍/g) all showed variation. Significant correlations between stem length and the number of branches (0.561) and number of branches versus number of flower clusters (0.638) were detected in the RIL F₇ population. Most agricultural characteristics and seed qualities showed a normal distribution with large variation, and transgressive segregation was observed in many descendants with characteristics to those of their parents. Daesil/Ipdeulkkae1 RIL F₇ populations could be useful for future QTL analysis as well as for intermediate breeding lines for high-quality perilla cultivars.

• Korean Journal of Medicinal Crop Science
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• v.12 no.6
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• pp.500-507
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• 2004

The establishment of an efficient protocol of plant regeneration from leaf explant cultures of Perilla spp. is reported. Regenerated shoots were obtained from leaf explant cultures on solid MS medium containing different concentrations of cytokinins and auxin. The effect of cytokonin and auxin differed depending on each acession. The combination treatments of high level of cytokinin and low level of auxin was more effective for plant regeneration in Perilla frutescens. The best concentration of sucrose was 3% for regeneration. Of spermidine, spermin and putrescine. treatments, the most effective treatment for plant regeneration was $10\;mg/{\ell}$ spermidine.

• 한국약용작물학회:학술대회논문집
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• 2009.05a
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• pp.167-168
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• 2009