KOREAN JOURNAL OF CROP SCIENCE (한국작물학회지)

The Korean Society of Crop Science (한국작물학회)
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Agricultural and Quality Characteristics in Recombinant Inbred Lines (RILs) Population in Perilla (Perilla frutescens)

들깨 대실/잎들깨1호 재조합 자식계통(RILs)의 농업적 특성 및 품질 분석

  • Park, Jae Eun (Department of Southern Area Crop Science, National Institute of Crop Science, Rural Development Administration) ;
  • Lee, Myoung Hee (Department of Southern Area Crop Science, National Institute of Crop Science, Rural Development Administration) ;
  • Oh, Ki Won (Department of Southern Area Crop Science, National Institute of Crop Science, Rural Development Administration) ;
  • Kim, Sungup (Department of Southern Area Crop Science, National Institute of Crop Science, Rural Development Administration) ;
  • Oh, Eunyoung (Department of Southern Area Crop Science, National Institute of Crop Science, Rural Development Administration) ;
  • Ha, Tae Joung (Department of Southern Area Crop Science, National Institute of Crop Science, Rural Development Administration) ;
  • Cho, Kwang-Soo (Department of Southern Area Crop Science, National Institute of Crop Science, Rural Development Administration) ;
  • Jung, Chan Sik (Department of Southern Area Crop Science, National Institute of Crop Science, Rural Development Administration) ;
  • Kim, Jung In (Department of Southern Area Crop Science, National Institute of Crop Science, Rural Development Administration)
  • 박재은 (농촌진흥청 국립식량과학원 남부작물부 밭작물개발과) ;
  • 이명희 (농촌진흥청 국립식량과학원 남부작물부 밭작물개발과) ;
  • 오기원 (농촌진흥청 국립식량과학원 남부작물부 밭작물개발과) ;
  • 김성업 (농촌진흥청 국립식량과학원 남부작물부 밭작물개발과) ;
  • 오은영 (농촌진흥청 국립식량과학원 남부작물부 밭작물개발과) ;
  • 하태정 (농촌진흥청 국립식량과학원 남부작물부 밭작물개발과) ;
  • 조광수 (농촌진흥청 국립식량과학원 남부작물부 밭작물개발과) ;
  • 정찬식 (농촌진흥청 국립식량과학원 남부작물부 밭작물개발과) ;
  • 김정인 (농촌진흥청 국립식량과학원 남부작물부 밭작물개발과)
  • Received : 2021.06.14
  • Accepted : 2021.08.17
  • Published : 2021.09.01
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Abstract

This study was conducted to obtain basal information for the development of perilla cultivars with improved quality. The F7 population of recombinant inbred lines (RILs) from a cross between the parents of Daesil for seeds and Ipdeulkkae1 for vegetables was used as the material. We evaluated several agricultural characteristics and seed quality. Variations were observed in most of the measurements; for example, stem length (ranging from 66.0 to 150.0 cm), number of branches (from 5 to 23), flower cluster length (ranging from 5.1 to 10.5 cm), number of flower clusters (from 17 to 131), a-linolenic acid content (from 54.2 to 64.1%), and functional compound content (rosmarinic acid 869.5~3,508.1 ㎍/g; luteolin 47.4~864.3 ㎍/g; apigenin 57.1~296.7 ㎍/g) all showed variation. Significant correlations between stem length and the number of branches (0.561) and number of branches versus number of flower clusters (0.638) were detected in the RIL F7 population. Most agricultural characteristics and seed qualities showed a normal distribution with large variation, and transgressive segregation was observed in many descendants with characteristics to those of their parents. Daesil/Ipdeulkkae1 RIL F7 populations could be useful for future QTL analysis as well as for intermediate breeding lines for high-quality perilla cultivars.

본 연구는 고품질의 들깨 신품종 육성을 위한 기초 자료로서 모본으로 종실용 품종인 '대실'과 부본으로 잎전용 품종인 '잎들깨1호'를 이용하여 교배된 재조합 집단(F1)을 양성하였고, F2세대부터 SSD법으로 계통을 전개한 고세대(F7) RIL집단 277계통들에 대해서 농업적 형질과 종실의 품질특성을 비교하였다. 재조합 집단의 주요 농업적 형질의 변이를 분석한 결과 경장은 66~150 cm, 유효분지수는 5~23개, 화방군장은 5.1~10.5 cm, 화방군수는 17~131개 및 화방군당삭수는 23~39개 등으로 분포하였다. 종실의 품질 특성을 분석한 결과 주요 지방산인 리놀렌산(C18:3)은 54.2~64.1%, 기능 성분인 rosmarinic acid는 869.5~3,508.1 ㎍/g, luteolin은 47.4~864.3 ㎍/g 및 apigenin은 57.1~296.7 ㎍/g 등으로 분포하였다. 조사된 대부분의 농업적 형질이 변이의 폭이 크면서 정규곡선 양상을 보였으며, 양친의 특성보다 우월한 특성을 가진 후대들이 많이 나타나는 초월분리 현상을 확인하였다. 이러한 결과는 F7 RIL 집단이 향후 QTL 분석뿐만 아니라 우수 품종 육성을 위한 중간 모본 양성에도 유용하게 활용될 수 있을 것으로 판단된다.

Keywords

Acknowledgement

본 논문은 농촌진흥청 연구사업(No. PJ01335502)의 지원에 의해 이루어진 결과의 일부이며 이에 감사드립니다.

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