• Food Science and Preservation
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• v.21 no.6
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• pp.878-884
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• 2014

Increased antimutagenic and in vitro anticancer effects were observed by the fermentation process during Kochujang manufacturing. In order to confirm the increased functionality, wheat grain, first fermented wheat grains (FFWG), second fermented wheat grains (SFWG), final fermented wheat grains (FiFWG), red pepper powder (RPP), and commercial Kochujang (CK) were prepared. Kochujang manufactured with final fermented wheat grains and red pepper powder were further fermented for 15 days and 30 days. The antimutagenic effects were determined by counting the number of revertants in Salmonella Typhimurium TA100 against N-methyl-N'-nitro-nitrosoguanidine (MNNG, 1.0mg/mL). The final fermented wheat grains (52% inhibition) showed higher antimutagenic effects than the wheat grain (34%), and the commercial Kochujang showed the highest antimutagenic effects (55%). We tested the inhibitory effect on the growth of HT-29 human colon carcinoma cells and AGS human gastric adenocarcinoma cells by using MTT (3-(4,5-dimethyl-thiazol)-2,5-diphenyltetrazolium bromide) assay. The results showed that increased fermentation process continually increased the growth inhibitory effect on both cancer cells. The further fermentation for 15 days of the Kochujang product also increased inhibitory growth of the AGS cancer cells. In conclusion, the methanol extract from fermented wheat grains and commercial Kochujang showed sequentially increased antimutagenic and in vitro anticancer activity, and thus the final commercial Kochujang revealed the highest effect.

• Korean Journal of Environmental Agriculture
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• v.15 no.4
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• pp.399-405
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• 1996

In the culture medium, the three antifungal fractions against P. capsici were separated by Sephadex G-25 column chromatography and Silica-gel chromatography. The substance A in white powder and the substance B in sticky oil were isolated by ethyl acetate : acetone mixture(7 : 3), and the substance C in yellow powder was isolated by chloroform : ethyl acetate mixture(95 : 5). The crude extract by ethyl acetate from the culture medium acidified to pH 2 was known to inhibit completely the growth of P. capsici at the level of $50mgkg^{-1}$. The substance A and B were known to be effective above the level of $5mgkg^{-1}$, and the substance C was effective above the level of $1mgkg^{-1}$. However, at the level of $20mgkg^{-1}$, the efficiency was in the order of A>C>B. It is apparent on a pot-experiment scale that the three substances effectively control Phytophthora blight of the red-pepper plant grown in the soil inoculated with P. capsici.

• Korean journal of applied entomology
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• v.47 no.1
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• pp.59-64
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• 2008

Insecticidal activity of 20 plant species against adults of Myzus persicae, female adults of Tetranychus urticae, and $2^{nd}\;or\;3^{rd}$ instar larvae of Plutella xylostella was evaluated in this study. Thirteen plant extracts showed over 80% acaricidal activity to T. urticae at the concentration of 1 %, while Capsicum annuum (hot-pepper seed), Inula helenium, and Acorus calamus revealed 82.8-86.2% acaricidal activities at 0.5%. Insecticidal activities of the plant extracts to M. persicae and P. xylostella were relatively low compare to the mite. Among the plant extracts, C. anuum and Brassica juncea revealed 48.6% and 42.9% respectively insecticidal activities to the aphid at the concentration of 0.5%. Extracts of C. anuum and Jeffersonia dubia provided 55% and 50% insecticidal activity against P. xylostella at 1%. And, the highest antifeeding activity of the insect was observed in the J. dubia extract as 79.4%. Results indicated that some plant extracts including hot pepper seed could be used as potential botanical pesticides for organic farming.

• The Korean Journal of Food And Nutrition
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• v.25 no.1
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• pp.9-16
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• 2012

The purposes of this study were to develop value-added sauce(ketchup) products with Korean advanced chile peppers (Capsicum annuum L.), determine their physicochemical characteristics, and conduct a sensory evaluation. American chile ketchup products were collected from American local favorites and analyzed based on their compositions. The Korean chile ketchup contained tomato paste(37.5%), Korean chile pepper(14.1%), sugar(14.8%), vinegar(14.1%), garlic(8.5%), herbs, plum extract, and oligosaccharide. Its physiochemical analysis showed: moisture $59.61{\pm}0.28%$, crude protein $2.18{\pm}0.11%$, crude lipid $1.99{\pm}0.04%$, crude ash $9.26{\pm}0.13%$, crude carbohydrate $26.97{\pm}0.48%$, reducing sugar $35.19{\pm}0.97%$, salt $3.04{\pm}0.04%$, acidity $2.22{\pm}0.01%$, pH $3.7{\pm}0.01$, and $^{\circ}brix\;36.3{\pm}0.14$. Korean chile ketchup showed higher overall acceptability compared to American local favorite chile ketchup. This result suggests the possibility for replacing chile ketchup products imported from foreign countries(USA and Europe).

• Journal of the Korean Society of Food Science and Nutrition
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• v.41 no.6
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• pp.727-732
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• 2012

The goal of this study is to determine the activities of antioxidants and antiglycation from the extracts of various Capsicum annum (known as pepper) ethanolic extract (CAE). We tested the extracts of Capsicum annum seeds and pericarps using 70% ethanol. The CAE showed antioxidant activities in a 2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) radical cation assay, 1,1-diphenyl-2-picrylhydrazyl free radical-scavenging assay, ferric-reducing antioxidant power assay, total flavonoid content, and total polyphenol content. Also, the physiological activities of CAE on glycation inhibition activities, anti-${\alpha}$-glucosidase activities, and tyrosinase activities were measured. As a result, green and red Capsicum annuum seeds show higher levels of antioxidant activities. In addition, the physiological activities are also more effective in the seeds than in the plant pericarps. A radar chart proves that antioxidants and physiological activities are more effective coming from the seeds. And the red Capsicum annuum seeds are more effective than the green ones.

• Korean journal of food and cookery science
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• v.28 no.3
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• pp.257-263
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• 2012

The purposes of this study were to develop value-added sauce (chili hot sauce) products with Korean advanced chili peppers (Capsicum annuum L.), to determine physicochemical characteristics of hot sauces, and to conduct a sensory evaluation of developed hot sauces. American chili hot sauce products were collected from the American local favorites, and were analyzed based on their compositions. The developed Korean hot sauce contained tomato (29.2%), onion (18.2%), sugar (11.5%), vinegar (10.9%), Korean chili peppers (11.0%), herbs, plum extract, and oligosaccharide, and it showed a higher overall acceptability, compared to that of the hot sauce samples. Its physiochemical analysis showed: salt $5.46{\pm}0.21%$, total acidity $6.04{\pm}0.04%$, pH $3.32{\pm}0.25$, and $^{\circ}brix$ $58.50{\pm}0.10$. This result suggests the possibility for developing a processed chili pepper products.

• The Korean Journal of Pesticide Science
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• v.15 no.4
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• pp.389-400
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• 2011

A high-performance liquid chromatographic (HPLC) method was developed to determine residues of phenothrin and silafluofen, known as synthetic pyrethroids, in agricultural commodities. Insecticide residues were extracted with acetone from representative samples of four crops which comprised rice, apple, pepper and cabbage. The extract was purified serially by liquid-liquid partition and Florisil column chromatography. For rice and pepper samples, acetonitrile/n-hexane partition was additionally adopted to remove nonpolar interferences. Reversed phase HPLC using an octadecylsilyl column was successfully applied to separate two phenothrin isomers and silafluofen from sample co-extractives. Intact parent compounds were sensitively detected by ultraviolet absorption at 226 nm. Recovery experiment at the quantitation limit validated that the proposed method could apparently determine phenothrin and silafluofen residues at 0.02 and 0.01 mg/kg, respectively. Mean recoveries of phenothrin and silafluofen from four crop samples fortified at three levels in triplicate were in the range of 82.4~109.8% and 83.7~109.8%, respectively. Relative standard deviations of the analytical method were all less than 10%, irrespective of crop types and spiking levels. A selected-ion monitoring (SIM) LC/mass spectrometry (MS) with electrospray ionization was provided to confirm the suspected residue of phenothrin, even though no sufficient ionization of silafluofen was obtained. Both phenothrin and silafluofen could be successfully confirmed by gas chromatography/MS SIM with electron impact at 70 eV. The proposed method is sensitive, repeatable and rapid enough to apply to officially routine inspection of agricultural products.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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• v.33 no.4
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• pp.267-275
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• 2015

Those land cover maps have widely been used in various fields, such as environmental studies, military strategies as well as in decision-makings. This study proposes a method to extract training data, automatically and classify the cover using ingle satellite images and national land cover maps, provided by the Ministry of Environment. For this purpose, as the initial training data, those three were used; the unsupervised classification, the ISODATA, and the existing land cover maps. The class was classified and named automatically using the class information in the existing land cover maps to overcome the difficulty in selecting classification by each class and in naming class by the unsupervised classification; so as achieve difficulty in selecting the training data in supervised classification. The extracted initial training data were utilized as the training data of MLC for the land cover classification of target satellite images, which increase the accuracy of unsupervised classification. Finally, the land cover maps could be extracted from updated training data that has been applied by an iterative method. Also, in order to reduce salt and pepper occurring in the pixel classification method, the MRF was applied in each repeated phase to enhance the accuracy of classification. It was verified quantitatively and visually that the proposed method could effectively generate the land cover maps.

• The Korean Journal of Soil Zoology
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• v.4 no.2
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• pp.81-85
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• 1999

Efficiencies of hand sorting, vermifuge application and combined methods of them for sampling earthworms in the field were compared. 1) hand sorting : sampling unit of soil (50 cm${\times}$50 cm${\times}$30 cm, width${\times}$length${\times}$depth) was dug out and earthworms were sorted by hands. 2) Vermifuge application : Solution of mustard, pepper or formalin was treated onto the soil surface of smpling unit (50 cm${\times}$50 cm, width${\times}$length). 3) Combined method : In a sampling unit of soil (50 cm${\times}$50 cm${\times}$30 cm, width${\times}$length), hand sorting was carried out into the depth of 10 cm or 15 cm and then vermifuge was treated. Earthworms were collected more in numbers by hand sorting than vermifuge application although it was much more time-consuming and labor intensive. Efficiency of mustard to extract earthworms was as same as formalin but much better than that of pepper. Combined method of hand sorting with mustard treatment was more efficient than vermifuge applications and other combined methods. And it could collect earthworms as many as hand sorting, but was much less time-consuming and labor-intensive than hand sorting. Thus combined method of hand sorting (to the depth of 15 cm) with mustard treatment could be recommended for the sampling of earthworms in the field.

• Analytical Science and Technology
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• v.21 no.6
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• pp.518-525
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• 2008

Mandipropamid is a new mandelamide-type fungicide to control foliar Oomycete pathogens in some vegetables. An analytical method was developed to determine mandipropamid residues in agricultural commodities using high-performance liquid chromatography (HPLC) and liquid chromatography/mass spectrometry (LC/MS). Mandipropamid was extracted with methanol from grape, tomato, green pepper, Chinese cabbage and potato samples. The extract was diluted with saturated sodium chloride solution and distilled water, and dichloromethane partition was followed to recover the mandipropamid from the aqueous phase. Florisil column chromatography was employed to further remove interfering co-extractives prior to HPLC analysis. Reverse-phased HPLC was successfully applied to determine mandipropamid in sample extracts with the detection at its ${\lambda}_{max}$ (223 nm). Overall recoveries of mandipropamid from fortified samples averaged $99.8{\pm}1.7$ (n=6), $89.3{\pm}5.3$ (n=6), $98.7{\pm}2.2$ (n=6), $99.7{\pm}6.8$ (n=6) and $91.1{\pm}3.1$ (n=6) for grape, tomato, green pepper, Chinese cabbage and potato, respectively. Limit of quantification of the method was 0.02~0.04 mg/kg for all samples. A LC/mass spectrometry with selected-ion monitoring was also provided to confirm the suspected residue. The proposed method was reproducible and sensitive enough to determine the terminal residue of mandipropamid in agricultural commodities.