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http://dx.doi.org/10.5806/AST.2008.21.6.518

Determination of mandipropamid residues in agricultural commodities using high-performance liquid chromatography with mass spectrometry  

Kwon, Chan Hyeok (Food Safety Evaluation Department, KFDA)
Chang, Moon Ik (Food Safety Evaluation Department, KFDA)
Im, Moo Hyeog (Food Safety Evaluation Department, KFDA)
Choi, Hoon (Food Safety Evaluation Department, KFDA)
Jung, Da I (Food Safety Evaluation Department, KFDA)
Lee, Su Chan (Food Safety Evaluation Department, KFDA)
Yu, Jin Young (Food Safety Evaluation Department)
Lee, Young Deuk (Division of Life and Environmental Science, Daegu University)
Lee, Jong Ok (Food Safety Evaluation Department, KFDA)
Hong, Moo Ki (Food Safety Evaluation Department, KFDA)
Publication Information
Analytical Science and Technology / v.21, no.6, 2008 , pp. 518-525 More about this Journal
Abstract
Mandipropamid is a new mandelamide-type fungicide to control foliar Oomycete pathogens in some vegetables. An analytical method was developed to determine mandipropamid residues in agricultural commodities using high-performance liquid chromatography (HPLC) and liquid chromatography/mass spectrometry (LC/MS). Mandipropamid was extracted with methanol from grape, tomato, green pepper, Chinese cabbage and potato samples. The extract was diluted with saturated sodium chloride solution and distilled water, and dichloromethane partition was followed to recover the mandipropamid from the aqueous phase. Florisil column chromatography was employed to further remove interfering co-extractives prior to HPLC analysis. Reverse-phased HPLC was successfully applied to determine mandipropamid in sample extracts with the detection at its ${\lambda}_{max}$ (223 nm). Overall recoveries of mandipropamid from fortified samples averaged $99.8{\pm}1.7$ (n=6), $89.3{\pm}5.3$ (n=6), $98.7{\pm}2.2$ (n=6), $99.7{\pm}6.8$ (n=6) and $91.1{\pm}3.1$ (n=6) for grape, tomato, green pepper, Chinese cabbage and potato, respectively. Limit of quantification of the method was 0.02~0.04 mg/kg for all samples. A LC/mass spectrometry with selected-ion monitoring was also provided to confirm the suspected residue. The proposed method was reproducible and sensitive enough to determine the terminal residue of mandipropamid in agricultural commodities.
Keywords
mandipropamid; fungicide; residue; agricultural commodity;
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