• Korean Journal of Medicinal Crop Science
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• v.9 no.2
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• pp.139-145
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• 2001

Peony has been cultivated as medicinal plant in East and also its beautiful flower was acknowledged over the world. In West, numerous peony varieties were developed for ornamental use but not in the country. Ninety two varieties of ornamental peony were introduced from U.S.A. and investigated in Uisong Medicinal Plant Experiment Station during $1997{\sim}1999$. For most of them, their growth habit were good in the country. Their flower colors were clearer and stems were thicker and stronger than domestic peonies. Some of them showed vigorous growth and made very high yield of root. Most of varieties showed proper content of paeoniflorin, medicinal component of peony, for medicinal use.

• Korean Journal of Medicinal Crop Science
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• v.15 no.1
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• pp.6-11
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• 2007

This study was conducted to identify physiologically active substances from leaves and stems of peony plant. MeOH extracts and column chromatography were employed to isolate active compounds and chemical structure were identified by IR, UV, Mass and NMR. The results obtained can be summerized as followings : Chemical structure of compound 1 was identified as oleanolic acid (white color form) of triterpenoid group, which is firstly identified from the above part of peony. Compound 2 was identified as kaempferol (yellow needle form) of flavonoid group, which was firstly identified from the root, leaf and stem of peony. Compound 3 was identified as methyl gallate (white power form) of phenol group, which was firstly identified from the above part of peony. Compound 4 was identified as astragalin (bright yellow needle form) of flavonoid group, that was firstly identified from the leaf and stem of peony. Compound 5 was identified as paeoniflorin (white color form) of monoterpene group, that was firstly identified from the above part of peony.

• The Journal of Korean Medicine
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• v.30 no.2
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• pp.88-103
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• 2009

Objectives: There is currently increased interest in the identification of natural antioxidant compounds derived from various plants. Peony Root (PR) is used worldwide for the treatment of many types of cardiovascular disease including atherosclerosis and hypertension. It has been used in Korean traditional medicine for the treatment of glycosuria, hypertension and cancer. However, to date, no studies concerning the antioxidant properties of PR have been conducted. Therefore, this study was conducted to evaluate the in vitro scavenging activity, inhibitory effect of LDL oxidation of pro-oxidant reactive species and anti-thrombosis effect in response to treatment with PR using various screening methods including biological and non-biological oxidants. Methods: In this study, the antioxidant activity of extract from PR was studied with in vitro methods by measuring the antioxidant activity by TEAC, measuring the scavenging effects on reactive oxygen species (ROS) [superoxide anion, hydroxyl radical] and on reactive nitrogen species (RNS) [nitric oxide and peroxynitrite] as well as measuring the inhibitory effect on $Cu^{2+}$-induced human LDL oxidation and the inhibitory effect on collagen-induced platelet aggregation. Results: The PR extracts were found to have a potent scavenging activity of oxidative stress [DPPH, superoxide anion, hydroxyl radical, nitric oxide and peroxynitrite, etc.] as well as an inhibitory effect on LDL oxidation and on platelet aggregation. Conclusions: The PR extracts have anti-oxidative and anti-atherosclerotic effects in vitro system, which can be used for developing pharmaceutical drugs against oxidative stress and atherosclerosis.

• Korean Journal of Medicinal Crop Science
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• v.6 no.3
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• pp.188-192
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• 1998

The correlations between growth charateristics and root yield were investigated in 74 peony lines collected from chief producing place in Korea. Among these peony lines, single flower type was 61% and the rest were double type. And pink flower was in majority. Stem length, stem diameter and number of stem per plant were $50{\sim}60cm$, $7{\sim}8mm$ and $5{\sim}6$, respectively. Generally, disease tolerance and root yield were better in single flower type peonies than double type. Among single flower type peonies, the correlation coefficients between each characteristics and root yield were as follows : number of stem $(0.676^{**})$, number of roots $(0.646^{**})$, stem length $(0.617^{**})$, root diameter $(0.590^{**})$ and root length $(0.443^{**})$. Correlation between root yield and paeoniflorin content was highly significant $(0.383^{**})$ among single flower type peonies and significant $(-0.382^*)$ among double flower type peonies. Correlation between stem diameter and root yield was not significant (0.066).

• KOREAN JOURNAL OF CROP SCIENCE
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• v.47 no.6
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• pp.459-464
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• 2002

This experiment was conducted to know the changes of bioactive component content in four-year-old peony (Paeonia lactiflora Pall.) root with various drying methods such as room temperature drying, $50^{\circ}C$ heat-air drying, room temperature drying after $80^{\circ}C$ boiling water treatment and freeze drying, and to establish the optimum drying method for high quality production of Paeoniae Radix. For this purpose, the contents of paeoniflorin, albiflorin and five phenolic compounds (gallic acid, benzoic acid, (+)-catechin, (-)-epicatechin and (+)-taxifolin 3-O-$\beta$-D-glucopyranoside) in peony root with different drying methods were analyzed by RP-HPLC. The contents of paeoniflorin, albiflorin and (+)-taxifolin 3-O-$\beta$-D-glucopyranoside at room temperature drying were higher than in the other drying methods and that of gallic acid at 8$0^{\circ}C$ boiling water treatment was the highest among that of all drying methods. In the case of freeze drying, the contents of (+)-catechin, benzoic acid and (-)-epicatechin were the highest among those of all drying methods. As increase of drying and treatment temperature, the contents of paeoniflorin, albiflorin, (+)-taxifolin 3-O-$\beta$-D-glucopyrano-side, (+)-catechin and benzoic acid were decreased.

• Korean Journal of Medicinal Crop Science
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• v.5 no.3
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• pp.237-242
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• 1997

The experiment was conducted to find causes of rooting failure in divided crown of peony cultivation. The results were as follows : In farmer s fields, damage rate of rooting failure in divided crown of peony was 32% as non-sprouting 5.6%, withering after sprouting 20.7%, and wilting after sprouting 5.7%, repectively. Damage degree in farmer s fields was followed, above 70% by 4%, 41 to 70% by 17%, 11 to 40% by 45% per total field area, respectively. It was caused by rooting failure. Damage rate of rooting failure as affected by different planting time was 18.2% for planting in autumn, 42.9% for planting in spring, and damage in divided crown was higher than in seedling. As periods to planting were prolonged, growth and yield were larger poorly, treatment with seminal-root sterilization and soil insecticide showed good growth and rooting but untreated control was very poor. Main cause of rooting failure in divided crown of peony was disease, Cylindrocarpon sp. and low quality of seeds.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 1999.04a
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• pp.18.2-18
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• 1999

• Korean Journal of Medicinal Crop Science
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• v.8 no.2
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• pp.151-156
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• 2000

Field experiments were conducted to investigate the yield and paeoniflorin content between healthy peony and blight plot of top part with every year during 3 years at June, July, August, September, separately. Stem length and diameter, No. of stem in the blight plot of peony were decreased in early in blight time compared with those in no blight growth. No. of flower buds per plant was 5.2, 4.6 in 3 year- grown and 4year-grown plots at late June blight compared with 11.5, 16.2 in no blight. No. of axillary flower was also similar to as above. Main root length and root diameter was most decreased in the plot of late June blight compared with healthy peony. No. of root more than 10mm of root diameter was small in order of blight time, namely Late June, July, August and September. The root dry yield of 4 year healthy peony was most high in 1,603kg per 10a, but it was lowed in 1,007kg in the plot lasted blight during 3years at late June. Paeoniflorin content was no different between 3year-grown and 4year-grown, but it was high in late June blight plot increased with the early in blight time.

• Journal of Microbiology and Biotechnology
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• v.18 no.9
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• pp.1537-1543
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• 2008

We tested Trichoderma harzianum as a biocontrol agent for Rhizoctonia solani AG2-1, using six natural antifungal materials to improve its efficacy. Among the six materials tested, peony (Paeonia suffruticosa) root bark (PRB) showed the strongest antifungal activity against R. solani AG2-1, and was not antagonistic to T. harzianum. Scanning electron microscopy showed that treatment with PRB extract resulted in shortened and deformed R. so/ani AG2-1 hyphal cells. The control of radish damping-off caused by R. so/ani AG2-1 was greatly increased by combined treatments of T. harzianum and PRB, as compared with either of the two treatments alone, with the control effect increased from 42.3-51.5% to 71.4-87.6%. The antifungal compound in PRB, which was isolated in chloroform and identified as paeonol by mass spectrometry, $^1H$ NMR, and $^{13}C$ NMR analyses, inhibited the growth of R. so/ani AG2-1 but not that of T. harzianum. Thus, PRB powder or extract may be used as a safe additive to T. harzianum to improve the control of the soil borne diseases caused by R. so/ani AG2-1.

• Korean Journal of Medicinal Crop Science
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• v.14 no.1
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• pp.19-22
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• 2006

Planting of peony, a perennial herb is usually planted in fall but the planting time can be postponed to next spring for other benefit such as land use, labour diversification and etc. This study was carried out to know the effect of planting time in spring on growth and yield of peony. Sprouting date in first year growth was earlier as planting time was later. Planting on March 10 showed best both top part and root growth and planting after that time resulted in worse growth. In planting on April 10, missing plant rate was 24% and accordingly root yield decreased to 43% comparing with that of March 10. Planting on March 10 resulted in thick and large number of root and so yield per l0a was 635 kg, the highest value among the other planting time in spring. The paeoniflorin content was not different significantly by planting time. These results suggested that March 10 was most appropriate for planting time in spring.