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Amendment with Peony Root Bark Improves the Biocontrol Efficacy of Trichoderma harzianum against Rhizoctonia solani  

Lee, Tae-Ok (Department of Agricultural Biotechnology and Center for Plant Molecular Genetics and Breeding Research, Seoul National University)
Khan, Zakaullah (Department of Agricultural Biotechnology and Center for Plant Molecular Genetics and Breeding Research, Seoul National University)
Kim, Sang-Gyu (Department of Agricultural Biotechnology and Center for Plant Molecular Genetics and Breeding Research, Seoul National University)
Kim, Young-Ho (Department of Agricultural Biotechnology and Center for Plant Molecular Genetics and Breeding Research, Seoul National University)
Publication Information
Journal of Microbiology and Biotechnology / v.18, no.9, 2008 , pp. 1537-1543 More about this Journal
Abstract
We tested Trichoderma harzianum as a biocontrol agent for Rhizoctonia solani AG2-1, using six natural antifungal materials to improve its efficacy. Among the six materials tested, peony (Paeonia suffruticosa) root bark (PRB) showed the strongest antifungal activity against R. solani AG2-1, and was not antagonistic to T. harzianum. Scanning electron microscopy showed that treatment with PRB extract resulted in shortened and deformed R. so/ani AG2-1 hyphal cells. The control of radish damping-off caused by R. so/ani AG2-1 was greatly increased by combined treatments of T. harzianum and PRB, as compared with either of the two treatments alone, with the control effect increased from 42.3-51.5% to 71.4-87.6%. The antifungal compound in PRB, which was isolated in chloroform and identified as paeonol by mass spectrometry, $^1H$ NMR, and $^{13}C$ NMR analyses, inhibited the growth of R. so/ani AG2-1 but not that of T. harzianum. Thus, PRB powder or extract may be used as a safe additive to T. harzianum to improve the control of the soil borne diseases caused by R. so/ani AG2-1.
Keywords
Biocontrol; damping-off; peony; Rhizoctonia solani; Trichoderma harzianum;
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