• Research in Plant Disease
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• v.30 no.2
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• pp.114-123
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• 2024

In July 2022, stem rot symptom was found in a peony plant grown in a pot under a greenhouse at Jinju, Gyeongnam Province, South Korea. Two fungal species were isolated from the infected peony stems and cultured on 1/2-strength potato dextrose agar for identification. The morphological characteristics of the fungal isolates were examined, and nucleotide sequences of the internal transcribed spacer region, β-tubulin and translation elongation factor 1-α were analysed. The pathogenicity of the two isolates was confirmed in detached peony leaves, according to Koch's postulates. To our knowledge, this is the report of Neopestalotiopsis clavispora and Sclerotinia sclerotiorum as the causal agents of peony stem rots. Antifungal activity of chemical fungicide propineb and rhizobacterium Bacillus siamensis H30-3 was shown against the two plant pathogenic fungi N. clavispora and S. sclerotiorum.Unidentified diffusible and volatile compounds from B. siamensis H30-3 could suppress in vitro mycelial growths of N. clavispora JJ 8-2-1 and S. sclerotiorum JJ 8-2-2.

• Korean Journal of Medicinal Crop Science
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• v.10 no.5
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• pp.392-398
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• 2002

Comparative analysis of paeoniflorin, albiflorin and phenolic compound contents as bioactive components of peony was performed by Reverse Phase-High Performance Liquid Chromatography (RPHPLC) using the four- year-old peony which were different plant parts and pretreatment, such as removing or unremoving the cork layer of peony root before drying. The contents of paeoniflorin, albiflorin, (+)-taxifolin $3-O-{\beta}-D-glucopyranoside$, (+)-catechin and (-)-epicatechin were the highest in rhizome part, but those of gallic acid and benzoic acid in the leaves were higher than other parts. The contents of albiflorin, gallic acid, benzoic acid and (-)-epicatechin in the cork layer were higher than in those of the core, but the contents of paeoniflorin, (+)-taxifolin $3-O-{\beta}-D-glucopyranoside$ and (+)-catechin in the core were higher than those in the cork layer. In general, the rhizome part of peony root has been used only propagation purpose, but this part contained high contents of bioactive component. Therefore, it is needed that medicinal application of rhizome part in peony root was firmly investigated. Also, In the use of peony root for medicinal purpose, the use of peony root with cork layer can be efficient way on the practical use of useful components and the reduction of labor for removing the cork layer.

• Korean Journal of Medicinal Crop Science
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• v.4 no.3
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• pp.231-235
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• 1996

The experiment was conducted to determine pollen morphology of Chinese peony and its germination. The results were obtained as following ; Pollen shpae was usually large ellipse and the pol­len germination rate of Eui-seong Peony line was 71% which was lower than that of other Peony lines. In a bud, the pollen began to be observed on $7{\sim}9days$ before flowering and as getting on for flowering time, normal pollen and germination rates were higher. The pollen germination and elongation beganat 30 minutes after incubation on artificial medium and were completed after $2{\sim}3\;hours$. As the storage time went on, the pollen life span was shortened. The germination rate was 51% at 8 days-storage of room temperature. The germination rate was 48% ad 43% at 95 day-storage in $0^{\circ}C$ and $-15^{\circ}C$, respectively.

• Korean Journal of Medicinal Crop Science
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• v.4 no.3
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• pp.187-192
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• 1996

This study was investigated to know about the stages of flower bud development and the effects of natural and artificial cold treatment on flowering of herbaceous peony. Developing buds of Paeonja lactㅑflora Pall. var. Taebaek were observed since Jun. 17 and peony plants were forced since Nov. 27 in the green house with two weeks interval, and other plants were forced after cold treatment in $5^{\circ}C$ for 1, 2, 4, 6weeks. Differentiation of vegetable part in peony buds was started in early June, and floral part was differentiated in September and their differentiation was continued to shooting in early spring. Buds of peony were sprouted and flowered when it was forced on Dec. 4. Days to shooting were decreased with delay of forcing time from early to late of December, significantly. Two weeks for cold treatment were enough to break dormancy of peony and days to shooting of the cold treated were significantly shorter than the untreated in the same forcing times

• Journal of the Korea Convergence Society
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• v.9 no.1
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• pp.261-268
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• 2018

The purpose of this study was to investigate the changes of serum immunoblobulin and DHEAs when 8 weeks of combined exercise and peony intake have been held to women in their 40s and 50s. 15 participants were chosen, divided into three groups, and they had undergone combined exercise with the level of 1RM 60~70%, HRmax 60~70%, three times a week for 60 minutes each. For the proper intake, 6g of peony were warmed in a double boiler with water 107ml, and the crude liquid was ingested 100ml each by the participants, everyday before breakfast, for 8 weeks. The following conclusions were obtained through these objectives and procedures. First, the amount of immunoglobulin has increased in two groups, the 8-week combined exercise group and the group which undergone both the peony intake and the combined exercise, but did not show the significant difference(p>.05). Second, the results of DHEAs showed an increas in two groups, the 8-week combined exercise group and the group which undergone both the peony intake and the combined exercise, but did not show the significant difference(p>.05). Therefore, the consumption of peony and complex exercise in this study did not positively affect the immune function and dementia prevention.

• Korean Journal of Medicinal Crop Science
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• v.4 no.4
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• pp.308-313
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• 1996

The appropriate drying method in medicinal compounds and color of peony root was that predrying at briquet fire $(40{\sim}60^{\circ}C)$ for 6 hours or more, and then hot air drying at $40^{\circ}C$ during 60 hours. But this method needs too much time in drying. And the method that drying at $40^{\sim}C$ with hot air drying has bad result in color. In case Peony was seld by cutting product, before cutting, the Peony root was retted for 30 min. and sealed for 12 hrs. is good for drying time, Peoniflorin content and commodity.

• Korean Journal of Medicinal Crop Science
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• v.3 no.1
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• pp.25-29
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• 1995

In order to obtain the basic information on cross breeding of peony, the growth and floral characteristics were investigasted for local collected strains in main cultvation area in Korea. Local peony strains were classified by early emergence lines and late emergence lines according to the emergence date. Flower color of single-petaling flower was varied with red, pink and white, on the other hand, red+red, pink+white, pink+red and pink+pink in double-petaling flower. Number of pistil of the most local peony strains was 3-4/flower, number of stamen was varied from 0 to more than 200 ea/flower, classifing by 4 groups according to the diameter of flower. Most of the cross combinations showed the cross comptibility, and number of follicle was 3-5 ea/flower, average number of seed was 15.6 ea/follicle, seed size was grouped : by small grains with less than 10g, medium grains with 10-15g and large grains with more than 15g of 100 seed weight.

• Korean Journal of Medicinal Crop Science
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• v.16 no.6
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• pp.421-426
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• 2008

Three year-old peony (Paeonia. lactiflora Pall. cv. Taebaek) was cultivated in green-house at Jan. 15, Feb. 15, or Mar. 15, respectively. The mean of temperature during the forcing cultivation was higher (air; $1.0{\sim}11.1^{\circ}C$, soil; $1.1{\sim}7.4^{\circ}C$) than that of open-field condition. From sprouting to flowering in peony cultivated at Jan. 15 was about 54 days, which is shorted the cut flower periods (ca. 26 days) compared with the open-field cultivation. However, earlier forcing cultivars were very susceptible to pathogens such as powdery mildew or gray mold. The yield in green house was also lower than in the open-field cultivation. The content of bioactive compounds such as paeoniflorin and albiflorin in green-house cultivars was similar that of open-field cultivars. These results showed the forcing cultivation time of peony at Feb. 15 in green-house was most desirable for commercialization.

• The Korean Journal of Mycology
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• v.45 no.2
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• pp.132-138
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• 2017

To obtain useful data on root rot in Korean ginseng, we performed phylogenetic analysis and pathogenicity test for Cylindrocarpon destructans isolated from peony. Cylindrocarpon destructans isolates from peony were proven to cause ginseng root rot. The isolate KACC44663 was identified as Ilyonectria robusta under the new classification system, which belongs to the I. radicicola species complex. This is the first report of the pathogenic isolate, which was isolated from another host plant, but not ginseng, that can cause root rot disease on ginseng in Korea.

• Archives of Pharmacal Research
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• v.28 no.7
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• pp.775-783
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• 2005

The ethanol extract of the peony root (Paeonia Lactiflora Pall, Paeoniaceae) as well as its major active components including gallic acid and methyl gallate were evaluated for their protective effects against free radical generation and lipid peroxidation. In addition, the protective effects against hydrogen peroxide-induced oxidative DNA damage in a mammalian cell line were examined. The ethanol extracts of the peony root (PREs) and its active constituents, gallic acid and methyl gallate, exhibited a significant free radical scavenging effect against 1,1-diphenyl-2-picryl hydrazine (DPPH) radical generation and had an inhibitory effect on lipid peroxidation, as measured by the level of malondialdehyde (MDA) formation. The PREs did not have any pro-oxidant effect. They strongly inhibited the hydrogen peroxide-induced DNA damage from NIH/3T3 fibroblasts, as assessed by single cell gel electrophoresis. Furthermore, the oral administration of 50% PRE (50% ethanol extract of peony root), gallic acid and methyl gallate potently inhibited the formation of micronucleated reticulocytes (MNRET) in the mouse peripheral blood induced by a $KBrO_3$ treatment in vivo. Therefore, PREs containing gallic acid and methyl gallate may be a useful antigenotoxic antioxidant by scavenging free radicals, inhibiting lipid peroxidation and protecting against oxidative DNA damage without exhibiting any pro-oxidant effect.