• Journal of Nutrition and Health
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• 제9권4호
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• pp.19-27
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• 1976

Perilla (frutescens) seed oil, which is widely used as a source of vegetable oil in Korea, contains a strikingly large amount (58.4% of total fatty acids) of polyunsaturated linolenic acid (18 : 3) which is one of the essential fatty acids. Our hypothesis was that vitamin E contained in this oil would not be enough to prevent peroxidation of this polyunsaturated oil. A comparative study was carried out using rats and chicks devided into seven groups with various diet combinations emphasizing fat sources for the period of four weeks. The level of fat in each diet was 15% and animals were fed ad libitum. Various diet combinations were as follows; perilla seed oil and sesame seed oil with and without vitamin E supplementation, tallow as a saturated fat source and perilla seed hull group (10% at the expense of carbohydrate). The fat constituents of control group were consisted of 50% vegetable oil and 50% animal fat. A few important findings are as follows: 1. Rats fed perilla seed oil lost their hair focally around the neck and suffered from a bad skin lesion at the same place. In chicks, yellow pigmentation both of feather and of skin was clearly observed only in groups fed perilla seed oil with or without vitamin E supplementation. The basis of biochemical mechanisms of this phenomena remains as an important research interest. 2. The mean value for hematocrit was significantly lower for the chicks fed perilla seed oil than for those fed control diet. This result seems to be attributable to the effect on the red cell membrane known as peroxidation-hemolysis of vitamin E deficiency. 3. The serum cholesterol level was higher for the rats fed perilla seed oil than for those fed control diet, whereas in chicks the group fed perilla seed oil showed lower value than the control group indicating that different animal species could vary in their responses to the same diet. 4. In pathological examinations, the sign of hepatic fibrosis was seen in the perilla seed hull group and it was noticeable that the level of hepatic RNA was significantly increased in the rat recovering from vitamin E deficiency. It is hoped that more detailed studies on perilla seed oil and hulls will soon be carried out in many aspects especially i) at various levels of fat in the diet, ii) in relation to dietary selenium level and iii) to find an optimum level of dietary essential fatty acids in terms of P/S ratio using various animal species. In the mean time, the public should be informed to preserve this particular oil with care to minimize fatty acid oxidation and should be discouraged from overconsuming this oil. This study was supported by UB (United Board) Research Grant (Graduate School, Yonsei University, Seoul, Korea)

• 한국산학기술학회논문지
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• 제19권2호
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• pp.185-191
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• 2018

본 연구는 유발된 정서가 뒤따르는 수 연산과제에 미치는 간섭현상의 종류와 크기를 확인해 보기 위해 수행되었다. 유발된 정서상태의 유지가 작업기억 요소 간 원활한 수행을 간섭할 수 있는 단계별 인지과제 처리 과정을 통해 자극유형 및 정서의 종류가 기초 연산(fundamental arithmetic operation)과제 수행에 미치는 영향을 알아 보았다. 피험자는 인구학적 변인에서 거의 차이가 없는 균질적인 대학생 집단으로 구성되었으며, 사전 연습시행에서 표정모방을 통해 유발된 정서를 유지하면서 후속되는 연산과제 수행 및 제시되는 답의 정오를 판단하도록 지침을 받았다. 분석결과, 자극유형 간에 간섭의 크기에서는 차이가 없었으나 유발된 정서의 종류 간에는 차이가 발견되었다. 유발된 정서의 시간지연 주효과가 계산과정의 어느 단계에 영향을 주었는지를 알아보기 위해, 자극 유형별로 정서 간 오류율과 지연 정반응률을 구하여 함께 분석하였는데, 긍정정서(기쁨조건)의 시간지연이 문장자극일 때에는 오류율 간 차이로, 그림자극일 때에는 지연 정반응률 간 차이로 나타남을 확인하였다. 이와 같은 결과는 유발된 긍정 정서의 영향이 부정정서(분노 및 슬픔 조건)에 비해 계산지연을 확대시키는 방향으로 나타났다는 점 외에 문장자극의 정서유발이 계산실패로 이어지기 쉬운 반면, 그림자극의 정서유발은 계산요동으로 이어지기 쉬운 특성이 있는 것으로 그 주효과의 영향이 분리될 수 있음을 시사한다고 하겠다.

• Radiation Oncology Journal
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• 제21권1호
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• pp.10-18
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• 2003

목적 : 양성 뇌종양에 대하여 분할 정위 방사선치료를 시행한 후의 임상 경과 및 방사선 반응 등을 추적하여 그 효과를 평가하고자 하였다. 대상 및 방법 : 1996년 3월부터 2002년 3월까지 양성 뇌종양으로 진단 받고 분할 정위 방사선치료를 받았던 36례를 대상으로 분석을 하였다. 대상 환자의 종양은 뇌하수체 선종 12례, 두개인두종 5례, 수막종 10례, 청신경초종 9례 이었다. 방사선치료는 종양의 위치, 종류, 크기에 따라 5~10회 분할 치료로 85~90%의 등선량 곡선에 25~35 Gy까지 조사하였다. 임상 추적관찰 기간은 2~74개월(중앙값 31개월)이었고, 방사선 추적 관찰 기간은 4~56개월(중앙값 21개 월)이었다. 결과 : 임상 증상이 있었던 35례 중 분할 정위 방사선치료 후 임상 증상의 호전은 13례(37.1%)에서 보였으며, 16례 (45.7%)에서 증상의 변화가 없었고 증상의 악화는 6례(17.2%)에서 관찰되었다. 뇌신경장애를 동반한 28례 중 7례(25%)에서 증상 개선을 보였다. 추적 방사선 검사를 시행한 33례 중 17례(51.5%)에서 종양 크기의 감소를 보였으며, 그 중 7례(21.2%, 뇌하수체 선종-2, 두개인두종-3, 수막종-1, 청신경초종-1)는 종양의 완전 소실을 보였다. 13례(39.4%)에서 종양 크기의 변화가 없었고, 3례(9.1%)는 종양 크기의 진행을 나타내었다. 급성 부작용은 11례(30.6%)에서 관찰되었다 결론 : 양성 뇌종양의 분할 정위 방사선치료는 심각한 부작용 없이 시행할 수 있는 안전하고 효과적인 치료법이었다. 그러나, 정확한 역할에 대해서는 좀 더 많은 환자와 장기간의 추적관찰이 필요할 것으로 사료되었다.

• Asian Pacific Journal of Cancer Prevention
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• 제17권3호
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• pp.1057-1060
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• 2016

Background: The tumor suppressor p53 is as a regulator of cell proliferation, apoptosis and many other biological processes as well as external and internal stress responses. Mdm2 SNIP309 is a negative regulator of 53. Therefore, this study aimed to determine the role of the Mdm2 SNIP 309 polymorphism in the gastric mucosal morphological patterns in patients with Helicobacter pylori associated gastritis. Materials and Methods: A prospective cross-sectional study was carried out from November 2014 through November 2015. Biopsy specimens were obtained from patients and infection was proven by positive histology. Gastric mucosa specimens were sent to the Molecular Genetics Unit, Institute of Medicine, Suranaree University of Technology where they were tested by molecular methods to detect the patterns of Mdm2 SNIP 309 polymorphism using the real-time PCR hybridization probe method. The results were analyzed and correlated with gastric mucosal morphological patterns by using C-NBI endoscopy. Results: A total of 300 infected patients were enrolled and gastric mucosa specimens were collected. In this study the percentage of Mdm2 SNIP 309 T/T homozygous and Mdm2 SNIP309 G/T heterozygous was 78% and 19 % respectively whereas Mdm2 SNIP309 G/G homozygous was 3%. Mdm2 SNIP 309 T/T homozygous and Mdm2 SNIP309 G/T heterozygosity correlated with type 1 to type 3 gastric mucosal morphological patterns (P<0.01) whereas Mdm2 SNIP309 G/G homozygous correlated with type 4 and type 5 (P<0.01). Conclusions: Our study finds the frequency of Mdm2 SNIP309 G/G in a Thai population is very low, and suggests that this can explain ae Thailand enigma. Types 1 to type 3 are the most common gastric mucosal morphological patterns according to the unique genetic polymorphism of MDM2 SNIP 309 in the Thai population.

• Asian Pacific Journal of Cancer Prevention
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• 제16권17호
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• pp.7781-7784
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• 2015

Background: The commonly held view of the tumor suppressor p53 is as a regulator of cell proliferation, apoptosis and many other biological processes as well as external and internal stress responses. Mdm2 SNIP309 is a negative regulator of p 53. Therefore, this study aimed to determine the correlation between the patterns of Mdm2 SNIP 309 and the inflammation grading of Helicobacter pylori associated gastritis in a Thai population. Materials and Methods: A cross-sectional study was carried out from November 2014 through June 2015. Biopsy specimens were obtained from infected patients and infection was proved by positive histology. The gastric mucosa specimens were sent to the Molecular Genetic Unit, Institute of Medicine, Suranaree University of Technology where they were tested by molecular methods to detect the patterns of Mdm2 SNIP 309 using the real-time PCR hybridization probe method. The results were analyzed and compared with the Updated Sydney classification. Results: A total of 100 infected patients were interviewed and gastric mucosa specimens were collected. In this study the percentage of Mdm2 SNIP 309 T/T homozygous and Mdm2 SNIP309 G/T heterozygous was 78% and 19 % respectively whereas Mdm2 SNIP309 G/G homozygous was 3%. Mdm2 SNIP 309 T/T homozygous and Mdm2 SNIP309 G/T heterozygous correlated with mild to moderate inflammation (P<0.01) whereas Mdm2 SNIP309 G/G homozygous correlated with severe inflammation (P<0.01). Conclusions: Our study found the frequency of Mdm2 SNP309 G/G in our Thai population to be very low, and suggests that this can explain to some extent the low incidence of severe inflammation and gastric cancer changes in the Thai population. Mild to moderate inflammation are the most common pathologic gradings due to the unique genetic polymorphism of Mdm2 SNIP 309 in the Thai population.

• 한국어병학회지
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• 제22권1호
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• pp.9-14
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• 2009

흑산도 근해의 가두리 양식장에서 아가미에 백색 결절을 주 증상으로 하는 조피볼락이 발견 되었다. 간과 신장에서는 병원균의 검출과 병리 조직학적인 변화가 전혀 발견되지 않았다. 아가미의 경우, epitheliocystis, Microcotyle sebastes 및 아가미 새변의 연골내에 미동정 생물(unknown aetiology)의 cyst가 확인되었다. Epitheliocystis의 cyst크기는 약 44.1-68.9 $\mu{m}$이었고 주로 새변의 끝과 중간부위에 기생하고 있었다. 그러나 새변내의 cyst형성에도 불구하고 숙주의 반응은 미약했다. M. sebastes는 2차새변에 고착기를 이용하여 달라붙어있었으며, 이로 인하여 상피세포의 증생이 발생하였다. 미동정 생물체의 경우, cyst는 호산성의 물질로 충만되어 있었고 cyst전체가 연골에 의해 싸여 있는 형태를 보였다. 하지만 이러한 cyst의 형성에도 불구하고 새변의 크기가 증대된 것 이외에는 별다른 병리학적인 증상은 보이지 않았다. 이들 기생충들의 의한 숙주세포의 조직반응은 미약하여 폐사는 유발되지 않았지만, 호흡과 삼투압 조절에는 장애가 있을 것으로 추정된다.

• Archives of Pharmacal Research
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• 제22권5호
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• pp.485-490
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• 1999

The mechanism of action of fish oil (FO), currently used in different chronic inflammatory conditions such as rheumatoid arthritis (RA), is not completely understood, although it is thought that it could alter the metabolism of endogenous autacoids. In addition, we hypothesized that the known capability of fatty acids (FA) of stabilizing serum albumin and perhaps other proteins, may be of pharmacological relevance considering that it is shared by other anti-rheumatic agents (e.g. nonsteroidal antiinflammatory drugs). Thus, we studied the effect of oral administration of FO and corn oil (CO), a vegetable oil with a different composition, on the stability of rat serum proteins, evaluated buy a classical in vitro method based on heat-induced protein denaturation. FO, and, to a lower extent, CO inhibited heat-induced denaturation of rat serum (RS): based on the inhibitory activity (EC50) of the major fatty acids against heat-induced denaturation of RS in vitro, it was possible to speculate the in vivo effects of palmitic acid (C16:0) and eicosapentaenoic acid (EPA, C20:5, n-3) may be more relevant than that of linolenic acid (C18:2). To better investigate this phenomenon, we extracted albumin from the serum of animals treated or not with FO with a one-step affinity chromatography technique, obtaining high purity rat serum albumin preparations (RSA-CTRL and RSA-FO), as judged by SDS-PAGE with Coomassie blue staining. When these RSA preparations were heated at $70^{\circ}C$ for 30 min, it was noted that RSA-FO was much more stable than RSA-CTRL, presumably due to higher number of long chain fatty acids (FA) such as palmitic acid or EPA. In conclusion, we provided evidences that oral administration of FO in the rat stabilizes serum albumin, due to an increase in the number of protein bound long chain fatty acids (e.g. palitic acid and EPA). We speculate that the stabilization of serum albumin and perhaps other proteins could prevent changes of antigenicity due to protein denaturation and glycosylation, which may trigger pathological autoimmune responses, suggesting that this action may be involved in the mode of action of FO in RA and other chronic inflammatory diseases.

• The Korean Journal of Physiology and Pharmacology
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• 제19권4호
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• pp.365-372
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• 2015

Aripiprazole (ARI) is a commonly prescribed medication used to treat schizophrenia and bipolar disorder. To date, there have been no studies regarding the molecular pathological and immunotoxicological profiling of aripiprazole. Thus, in the present study, we prepared two different formulas of aripiprazole [Free base crystal of aripiprazole (ARPGCB) and cocrystal of aripiprazole (GCB3004)], and explored their effects on the patterns of survival and apoptosis-regulatory proteins under acute toxicity and cytotoxicity test conditions. Furthermore, we also evaluated the modulatory activity of the different formulations on the immunological responses in macrophages primed by various stimulators such as lipopolysaccharide (LPS), pam3CSK, and poly(I:C) via toll-like receptor 4 (TLR4), TLR2, and TLR3 pathways, respectively. In liver, both ARPGCB and GCB3004 produced similar toxicity profiles. In particular, these two formulas exhibited similar phospho-protein profiling of p65/nuclear factor $(NF)-{\kappa}B$, c-Jun/activator protein (AP)-1, ERK, JNK, p38, caspase 3, and bcl-2 in brain. In contrast, the patterns of these phospho-proteins were variable in other tissues. Moreover, these two formulas did not exhibit any cytotoxicity in C6 glioma cells. Finally, the two formulations at available in vivo concentrations did not block nitric oxide (NO) production from activated macrophage-like RAW264.7 cells stimulated with LPS, pam3CSK, or poly(I:C), nor did they alter the morphological changes of the activated macrophages. Taken together, our present work, as a comparative study of two different formulas of aripiprazole, suggests that these two formulas can be used to achieve similar functional activation of brain proteins related to cell survival and apoptosis and immunotoxicological activities of macrophages.

• Journal of Microbiology and Biotechnology
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• 제28권12호
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• pp.2095-2105
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• 2018

In our previous studies, we have identified several in vivo-induced antigens and evaluated their potential as subunit vaccine candidates in a murine model, in which the recombinant protein GalT showed the most potent immunogenicity and immunoprotective efficacy against Actinobacillus pleuropneumoniae. To exploit a more efficient way of delivering GalT proteins, in this study, we employed the widely studied E. coli outer membrane vesicles (OMVs) as a platform to deliver GalT protein and performed the vaccine trial using the recombinant GalT-OMVs in the murine model. Results revealed that GalT-OMVs could elicit a highly-specific, IgG antibody titer that was comparable with the adjuvant GalT group. Significantly higher lymphocyte proliferation and cytokines secretion levels were observed in the GalT-OMVs group. 87.5% and 50% of mice were protected from a lethal dose challenge using A. pleuropneumoniae in active or passive immunization, respectively. Histopathologic and immunohistochemical analyses showed remarkably reduced pathological changes and infiltration of neutrophils in the lungs of mice immunized with GalT-OMVs after the challenge. Taken together, these findings confirm that OMVs can be used as a platform to deliver GalT protein and enhance its immunogenicity to induce both humoral and cellular immune responses in mice.

• 감성과학
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• 제24권2호
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• pp.39-48
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• 2021

국제통증연구학회(IASP)에 따르면, 신경병증성 통증은 정상 조건에서 중추신경계에 유해한 정보를 전달하는 신경계 기능 장애로 특징 지워진다. 이런 통증은 말초 혹은 중추 신경계에 확인 가능한 병변이 있는 질환과 어떠한 신경에도 병변이 없는 상태에서 발생하는 상황으로 나누어 볼 수 있다. 두 가지 상황 모두 장기적이고 만성적인 변화과정을 겪게 되며, 결과적으로 신경계가 부적절하게 적응하여 치유되기 어려운 만성통증 증후군으로 발전할 수 있다. 그러나 이러한 통증 치료는 진단에서부터 치료까지의 과정이 어려운 탓에 현재까지도 특별한 해결방안이 부족한 실정이다. 최근 자기공명영상(fMRI), 양전자방출단층촬영법(PET), 광영상(optical imaging) 등 영상분석기술이 발달함에 따라 통증을 유발할 수 있는 유해 자극에 대한 중추신경계의 반응을 영상화하는 연구가 증가하고 있다. 이러한 영상 기법들을 통해 통증을 해석하고 처리하는 뇌 영역에서 시냅스 간 가소성 변화가 일어나고 있음을 확인하였으며, 신경병증성 통증을 비롯한 만성통증과 학습과의 상호 작용을 이해하는 데 많은 도움을 주고 있다. 본 연구는 병리적 통증의 기전과 통증 자극에 따른 뇌의 구조적, 기능적 변화에 대해 최근까지 밝혀진 연구들을 소개하고자 한다. 만성적 통증의 정의와 발생기전을 되짚고 새로운 연구 동향을 살펴보는 것은 통증을 완화할 수 있는 방안을 강구하는 데 도움이 될 것으로 사료된다.