• Microbiology and Biotechnology Letters
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• v.25 no.1
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• pp.30-36
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• 1997

To genetically breed powerful multifunctional antagonistic bacteria, the urease gene of alkalophilic Bacillus pasteurii was transferred into Bacillus subtilis YB-70 which had been selected as a powerful biocontrol agent against root-rotting fungus Fusarium solani. Urease gene was inserted into the HindIII site of pGB215-110 and designated pGU266. The plasmid pGU266 containing urease gene was introduced into the B. subtilis YB-70 by alkali cation transformation system and the urease gene was very stably expressed in the transformant of B. subtilis YB-70(pGU266). The optimal conditions for the transfomation were also evaluated. From the in vitro antibiosis tests against F. solani, the antifungal activity of B. subtilis YB-70 containing urease gene was much efficient than that of the non-transformed strain. Genetic improvement of B. subtilis YB-70 by transfer of urease gene for the efficient control seemed to be responsible for enhanced plant growth and biocontrol efficacy by combining its astibiotic action and ammonia producing ability.

• Toxicological Research
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• v.11 no.2
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• pp.261-266
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• 1995

Age-related change in the frequency of spontaneous sister chromatid exchange (SCE) and chromosornal aberrations were investigated in bone marrow cells of accelerated senescence-resistant mice (SAM R1) and senescence accelerated ones (SAM P1). And the effect of chronic treatment of ginseng extract (Panax ginseng C.A. Meyer) on these chromosomal abnormalities was tested in SAM P1. SCE frequency in the cells was progressively increased with age in both mice, but it was consistently higher in SAM P1 than in SAM R1 at all corresponding age. Chromosomal aberrations were, however, not significantly changed with age except that it was slightly increased in only aged SAM P1. Interestingly, the rate of these genetic instabilities in SAM P1 was remarkably retarded by long-term administration of ginseng water extract (0.05% in drinking water). These results suggest that frequency of spontaneous SCE in bone marrow cells increase in parallel with senescence of the mice, and SAM P1 is in the condition of being more exposed than SAM R1 to DNA damaging factors. These also indicate that long-term treatment of ginseng may reduce the genetic damage.

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2001.05a
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• pp.265-266
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• 2001

PCDDS and PCDFs are very stable chcmicals and have very long residence times in the environment and in organisms, including humans. Their hydrophobicity promotes accumulation in sediments and organisms, resulting in high concentrations in both sediments and organisms. Among toxicological effects reports arc teratogenicity, reduce reproduction, liver toxicity, decreased growth rate and behavioral changes (Zeise et al., 1990; Huff, 1992). PCDDs/DFs are inadvertently produced from various combustion sources and manufacturing processes, such as municipal solid waste incineration (Olie et at., 1977), motor vehicles (Marklund et al., 1987), steel mills (Tysklind et al., 1989), and chemical production processes (Hutzinger et al., 1985). (omitted)

• Research in Community and Public Health Nursing
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• v.29 no.3
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• pp.257-266
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• 2018

Purpose: The study has been conducted to identify factors affecting the participation in leisure activities by elderly women living alone. Methods: Data were collected through a questionnaire survey among 150 elderly women in Seoul and Gyeong-gi Province. The measurement tools that were used include participation in leisure activities, perceived health status, Geriatric Depression Scale Short Form-Korea (GDSS-K), psychological well-being scale, and loneliness scale. The data were analyzed by using the Pearson correlation coefficients, t-test, analysis of variance ANOVA, and stepwise multiple regression with the SPSS/windows version 21.0 program. Results: The analysis shows that the affection for participation in leisure activities of the elderly women living alone who participated in the questionnaire survey is significant (F=14.6, p<.001). The value of the adjusted $R^2$ is 0.55, which accounts for the explanatory power of 55.4%. The predictor that has been found to have the greatest influence on the participation in leisure activities by the elderly women living alone include perceived health status, followed by monthly allowance (10,000 won), psychological well-being, economic status, religion, depression, family structure, and loneliness. Conclusion: The results suggest that in developing nursing interventions and practice for the participation in leisure activities by elderly women living alone, perceived health status, psychological well-being, depression, and loneliness should be considered.

• Journal for History of Mathematics
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• v.29 no.5
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• pp.257-266
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• 2016

This paper is a sequel to our paper [3]. Although polynomials in the tianyuanshu induce perfectly the algebraic structure of polynomials, the tianyuan(天元) is always chosen by a specific unknown in a given problem, it can't carry out the role of the indeterminate in ordinary polynomials. Further, taking the indeterminate as a variable, one can study mathematical structures of polynomials via those of polynomial functions. Thus the theory of polynomials in East Asian mathematics could not be completely materialized. In the previous paper [3], we show that Jeong Yag-yong disclosed in his Gugo Wonlyu(勾股源流) the mathematical structures of Pythagorean polynomials, namely polynomials p(a, b, c) where a, b, c are the three sides gou(勾), gu(股), xian(弦) of a right triangle, respectively. In this paper, we show that Jeong obtained his results through his recognizing Pythagorean polynomials as polynomial functions of three variables a, b, c.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.16 no.1
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• pp.576-584
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• 2015

This research studiedthe correlation between dental implant patients' oral hygiene behaviors and satisfaction. By doing so, this study seeks to provide the basic data for the development of diagonal and educational programs to improve implant prosthesis maintenance and satisfaction. The data was collected from January 7 to June 30, 2014, analyzed by SAS (ver 9.2) and for this research, 6 dental hospitals and clinics in Daegu, Busan and Jinju, South Korea were examined for their implant patients receiving treatment. As a result, a total of 266 sets of data were investigated herein to reach the following findings: 1. The survey on status of dental implant prosthesis use and maintenance knowledge found 1.41 on average out of the total score of 3. 2. In the survey on implant prosthesis maintenance after putting in, the dental scaling cycle was found to be 'none' (63.2%). 3. In the survey on implant use status and satisfaction with their implant prosthesis, the masticatory function (p=0.001) were found to be significantly higher in the group using such oral-hygiene goods. 4. In the correlation analysis among one's knowledge on dental implant maintenance, discomfort and satisfaction, it was found that the more they used oral-hygiene supplementary goods (${\beta}=0.095$), the more they had knowledge on implant maintenance (${\beta}=0.069$) and the more they experienced oral health education (${\beta}=0.032$), the higher their satisfaction levels were. It is deemed that, for enhanced satisfaction of dental implant patients, their maintenance behaviors need to be further improved through oral health educational programs.

• Tuberculosis and Respiratory Diseases
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• v.73 no.5
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• pp.266-272
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• 2012

Background: Limited data on the incidence and clinical characteristics of adult pertussis infections are available in Korea. Methods: Thirty-one hospitals and the Korean Centers for Disease Control and Prevention collaborated to investigate the incidence and clinical characteristics of pertussis infections among adults with a bothersome cough in non-outbreak, ordinary outpatient settings. Nasopharyngeal aspirates or nasopharyngeal swabs were collected for polymerase chain reaction (PCR) and culture tests. Results: The study enrolled 934 patients between September 2009 and April 2011. Five patients were diagnosed as confirmed cases, satisfying both clinical and laboratory criteria (five positive PCR and one concurrent positive culture). Among 607 patients with cough duration of at least 2 weeks, 504 satisfied the clinical criteria of the US Centers for Disease Control and Prevention (i.e., probable case). The clinical pertussis cases (i.e., both probable and confirmed cases) had a wide age distribution ($45.7{\pm}15.5$ years) and cough duration (median, 30 days; interquartile range, 18.0~50.0 days). In addition, sputum, rhinorrhea, and myalgia were less common and dyspnea was more common in the clinical cases, compared to the others (p=0.037, p=0.006, p=0.005, and p=0.030, respectively). Conclusion: The positive rate of pertussis infection may be low in non-outbreak, ordinary clinical settings if a PCR-based method is used. However, further prospective, well-designed, multicenter studies are needed.

• Biomolecules & Therapeutics
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• v.25 no.3
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• pp.266-271
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• 2017

Synthetic cannabinoids are one of most abused new psychoactive substances. The recreational use of abused drug has aroused serious concerns about the consequences of these drugs on infection. However, the effects of synthetic cannabinoid on resistance to tetanus toxin are not fully understood yet. In the present study, we aimed to determine if the administration of synthetic cannabinoids increase the susceptibility to tetanus toxin-induced motor behavioral deficit and functional changes in cerebellar neurons in mice. Furthermore, we measured T lymphocytes marker levels, such as CD8 and CD4 which against tetanus toxin. JWH-210 administration decreased expression levels of T cell activators including cluster of differentiation (CD) $3{\varepsilon}$, $CD3{\gamma}$, CD74p31, and CD74p41. In addition, we demonstrated that JWH-210 induced motor impairment and decrement of vesicle-associated membrane proteins 2 levels in the cerebellum of mice treated with tetanus toxin. Furthermore, cerebellar glutamatergic neuronal homeostasis was hampered by JWH-210 administration, as evidenced by increased glutamate concentration levels in the cerebellum. These results suggest that JWH-210 may increase the vulnerability to tetanus toxin via the regulation of immune function.

• 대한공업교육학회지
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• v.34 no.2
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• pp.248-266
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• 2009

The purpose of the research was to find out the difference in students' academic achievement in Technology Education between students with a lecture, those who perform a content-related project after a lecture, and those who carry out a content-related project without a lecture. The results of this study are as follows. First, taking advantage of both a lecture and project-based lesson led to better achievement than using only a project in Technology Education subject of an academic high school in academic achievement in 'knowledge' area. I infer that it is because they reviewed what they had learned in a lecture and the preparation and practice of the project caused them to memorize it. Second, there was not a meaningful difference in academic achievement in 'understanding' area among the group with a lecture, the one with both a lecture and a project, and the one with only a project. However, considering the comparison of averages and the p-value of F-test, I can deduce that the test outcome influences students with a lecture and a project positively in terms of academic achievement. Third, there was not a meaningful difference in the academic achievement in 'adaptation' area among the group with a lecture, the one with a lecture and a project, and the one with a project. I can conclude that those results are because the difficulty level of evaluation was high and they produced a model just by copying textbook contents.