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Investigation of bacteria in indoor air of a greenhouse for button mushroom cultivation (양송이 재배사 공기 중에 존재하는 세균조사)

  • Kwon, Hyuk Woo;Choi, Min Ah;Oh, Youn-Lee;Kong, Won-Sik;Kim, Seong Hwan
    • Journal of Mushroom
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    • v.13 no.1
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    • pp.26-29
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    • 2015
  • Since button mushroom (Agaricus bisporus) cultivation is performed in closed environment, the understanding of indoor environment becomes essential for the quality and quantitative production of the greenhouse-grown mushroom. To generate information on indoor environmental factors affecting on fruiting body quality, we investigated temperature, humidity, and bacterial concentration and species in a greenhouse located in Buyeo, Chungnam Province. Temperature and humidity were recorded as $19.75{\pm}0.35^{\circ}C$ and $87{\pm}3.67%$, respectively. The total concentration of bacteria was measured as $3.84{\times}10^3CFU/M^3$. Advenella kashmirensis, Bacillus vietnamensism, B. licheniformis, Burkholderia sordidicola, Fictibacillus phosphorivorans, Lysobacter daejeonensis, Microbacterium esteraromaticum, Pseudomonas aeruginosa, P. protegens, P. gessardii, P. mosseli were identified from indoor air of the greenhouse.

Gas Transport Properties of Soluble Polyimides Containing Alicyclic Dianhydride (지환족 다이안하이드라이드를 포함하는 용해성 폴리이미드의 기체투과특성)

  • Kim, Eun Hee;Park, Chae Young;Kim, Jeong-Hoon
    • Membrane Journal
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    • v.24 no.2
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    • pp.100-106
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    • 2014
  • In this work, soluble polyimides were synthesized and characterized from 5-(2,5-dioxotetrahydrofuryl)-3-methyl-3-cyclohexene-1,2-dicarboxylic anhydride (DOCDA) and two diamines such as 4,4'-diaminodiphenylether (ODA), 1,4-phenylenediamine (p-PDA). Their thermal properties were analyzed with differential scanning calorimeter (DSC). The gas permeability coefficients (P) and ideal selectivity for $CH_4$ and $CO_2$ of the prepared polyimide membranes were measured with a time-lag apparatus. DOCDA-ODA, DOCDA-p-PDA showed good permeability and selectivity; the permeabilities of $CO_2$ was 6.10, 0.74 barrers and the selectivity of $CO_2/CH_4$ were 67.03, 46.25, respectively. Therefore, DOCDA-ODA showed good possibility as gas separation membrane.

Properties of a bilirubin oxidase from Penicillium sp. LAM 91-89 (Penicillium sp. LAM 91-89가 생산하는 bilirubin oxidase의 특성)

  • Yi, Dong-Heui;Lee, Dong-Ho;Kim, Jung-Bae;Lee, No-Woon
    • Applied Biological Chemistry
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    • v.36 no.3
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    • pp.158-162
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    • 1993
  • A bilirubin oxidase produced by Penicillium sp. strain LAM 91-89 was purified and partially characterized. The enzyme was purified about 70 folds from culture broth by ethanol precipitation, first and second Sephadex G-200 column chromatography with overall yield of 12%. The molecular weight of the enzyme was estimated to be 53,000 dalton by SDS-PAGE. The optimum pH and temperature was 8.5 and $40^{\circ}C$, respectively. The enzyme was stable in the pH range $6{\sim}10$ and below $40^{\circ}C$. Activity of the enzyme was increased by the addition of $Mg^{2+}$ but was gretly inhibited by $Ag^+,\;Hg^{2+},\;Mn^{2+},\;Pb^{2+}$, iodoacetate, p-chloromercurobenzoic acid and sodium dodecyl sulfate. Among various substrates, bilirubin was favorably reacted and $K_m$ value for bilirubin was $6.67\;{\mu}mole$.

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Mention Detection with Pointer Networks (포인터 네트워크를 이용한 멘션탐지)

  • Park, Cheoneum;Lee, Changki
    • Journal of KIISE
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    • v.44 no.8
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    • pp.774-781
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    • 2017
  • Mention detection systems use nouns or noun phrases as a head and construct a chunk of text that defines any meaning, including a modifier. The term "mention detection" relates to the extraction of mentions in a document. In the mentions, a coreference resolution pertains to finding out if various mentions have the same meaning to each other. A pointer network is a model based on a recurrent neural network (RNN) encoder-decoder, and outputs a list of elements that correspond to input sequence. In this paper, we propose the use of mention detection using pointer networks. Our proposed model can solve the problem of overlapped mention detection, an issue that could not be solved by sequence labeling when applying the pointer network to the mention detection. As a result of this experiment, performance of the proposed mention detection model showed an F1 of 80.07%, a 7.65%p higher than rule-based mention detection; a co-reference resolution performance using this mention detection model showed a CoNLL F1 of 52.67% (mention boundary), and a CoNLL F1 of 60.11% (head boundary) that is high, 7.68%p, or 1.5%p more than coreference resolution using rule-based mention detection.

Naloxone Postconditioning Alleviates Rat Myocardial Ischemia Reperfusion Injury by Inhibiting JNK Activity

  • Xia, Anzhou;Xue, Zhi;Wang, Wei;Zhang, Tan;Wei, Tiantian;Sha, Xingzhi;Ding, Yixun;Zhou, Weidong
    • The Korean Journal of Physiology and Pharmacology
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    • v.18 no.1
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    • pp.67-72
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    • 2014
  • To investigate the alteration of c-Jun N-terminal kinase (JNK) activity after myocardial ischemia reperfusion injury (MIRI) and further explore the effect of naloxone postconditioning on MIRI. Forty male Sprague Dawley rats were randomly divided into five groups: sham operation (sham, n=8); ischemia reperfusion (IR, n=8); IR+naloxone 0.5 mg/kg (Nal L, n=8); IR+naloxone 1.0 mg/kg (Nal M, n=8); IR+naloxone 2.0 mg/kg (Nal H, n=8). Pathological changes of myocardial tissue were visualized by HE staining. The expression of p-JNK, and the apoptosis of cardiomyocytes were investigated with Western blotting and the TUNEL assay, respectively. Irregular arrangement and aberrant structure of myocardial fibers, cardiomyocytes with granular or vacuolar degeneration, and inflammatory cells infiltrating the myocardial interstitial regions characterized MIRI in the IR group. Signs of myocardial injury and inflammatory infiltration were less prominent in the Nal-treated groups. The expression of p-JNK in the sham group and in all Nal-treated groups was significantly lower than that in the IR group (p<0.01). The apoptosis index of cardiomyocytes in the IR group was significantly higher than in the sham group (p<0.01). The apoptosis indices of cardiomyocytes in all Nal-treated groups were significantly reduced to 55.4%, 26.2%, and 27.6%, respectively, of the IR group (p<0.01). This study revealed that Naloxone postconditioning before reperfusion inhibits p-JNK expression and decreases cell apoptosis, thus alleviating MIRI.

Relationships of Stigma, Family Support, and Quality of Life in Tuberculosis Patients (결핵환자의 스티그마, 가족지지와 삶의 질의 관계)

  • Park, Su-Jung;An, Minjeong;So, Hyang-Sook
    • The Journal of the Korea Contents Association
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    • v.15 no.9
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    • pp.285-294
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    • 2015
  • Purpose: The purpose of this study was to identify the relationships between stigma, family support, and quality of life in tuberculosis patients. Methods: The participants were 122 who completed a questionnaire. The data were analyzed using descriptive statistics, t-test, and ANOVA. Results: The score of stigma I(community perspectives) was $18.67{\pm}7.48$ and the score of stigma II(patients perspectives) was $14.84{\pm}6.93$. The score of those with family support was $30.81{\pm}4.16$. The score of QOL was $102.22{\pm}13.72$. The findings showed significant positive relationships between stigma I and stigma II and between family support and QOL(r = .753, p < .001; r = .314, p < .001, respectively) and negative relationship between stigma II and QOL(r = -.250, p = .005). Conclusion: Further studies are needed to develop and evaluate the nursing interventions to support patients with tuberculosis, reducing their level of stigma and improving their quality of life.

Connexin32 inhibits gastric carcinogenesis through cell cycle arrest and altered expression of p21Cip1 and p27Kip1

  • Jee, Hyang;Lee, Su-Hyung;Park, Jun-Won;Lee, Bo-Ram;Nam, Ki-Taek;Kim, Dae-Yong
    • BMB Reports
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    • v.46 no.1
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    • pp.25-30
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    • 2013
  • Gap junctions and their structural proteins, connexins (Cxs), have been implicated in carcinogenesis. To explore the involvement of Cx32 in gastric carcinogenesis, immunochemical analysis of Cx32 and proliferation marker Ki67 using tissue-microarrayed human gastric cancer and normal tissues was performed. In addition, after Cx32 overexpression in the human gastric cancer cell line AGS, cell proliferation, cell cycle analyses, and $p21^{Cip1}$ and $p27^{Kip1}$ expression levels were examined by bromodeoxyuridine assay, flow cytometry, real-time RT-PCR, and western blotting. Immunohistochemical study noted a strong inverse correlation between Cx32 and Ki67 expression pattern as well as their location. In vitro, overexpression of Cx32 in AGS cells inhibited cell proliferation significantly. $G^1$ arrest, up-regulation of cell cycle-regulatory proteins $p21^{Cip1}$ and $p27^{Kip1}$ was also found at both mRNA and protein levels. Taken together, Cx32 plays some roles in gastric cancer development by inhibiting gastric cancer cell proliferation through cell cycle arrest and cell cycle regulatory proteins.

Analysis of the electrical characteristics of the novel TIGBT with additional pMOS (새로운 구조의 pMOS 삽입형 TIGBT의 전기적 특성 분석)

  • Lee, Hyun-Duck;Won, Jong-Il;Yang, Yil-Suk;Koo, Yong-Seo
    • Journal of IKEEE
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    • v.14 no.1
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    • pp.55-64
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    • 2010
  • In this paper, we proposed the novel TIGBT with an additional p-type MOS structure to achieve the improved trade-off between turn-off and on-state voltage drop(Vce(sat)). These low on-resistance and the fast switching characteristics of the proposed TIGBT are caused by an enhanced electron current injection efficiency which is caused by additional p-type MOS structure. In the simulation result, the proposed TIGBT has the lower on state voltage of 1.67V and the shorter turn-off time of 3.1us than those of the conventional TIGBT(2.25V, 3.4us).

Effects of feeding different proportions of silver leaf desmodium (Desmodium uncinatum) with banana (Musa paradisiaca) leaf on nutrient utilization in Horro sheep fed a basal diet of natural grass hay

  • Chali, Diriba;Nurfeta, Ajebu;Banerjee, Sandip;Eik, Lars Olav
    • Asian-Australasian Journal of Animal Sciences
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    • v.31 no.9
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    • pp.1449-1457
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    • 2018
  • Objective: The objective was to evaluate feed intake, digestibility, body weight change and carcass characteristics of sheep fed a basal diet of hay supplemented with banana leaves and silver leaf desmodium. Methods: Thirty yearling lambs with an average initial body weight of $15.85{\pm}1.6kg$ were grouped into six blocks of five rams in each block. The treatments were: hay alone (T1), hay+100% banana leaf (T2), hay+67% banana leaf+33% desmodium leaf (T3), hay+33% banana leaf+67% desmodium leaf (T4), andhay+100% desmodium leaf (T5). Three hundred grams of treatment diets were offered daily on as fed basis. The feeding and digestibility trials lasted for 84 and 7 days, respectively, followed by carcass evaluation. Results: The total dry matter (DM) intake for T3, T4, and T5 were greater (p<0.05) than those fed T1 and T2 diets. The lowest (p<0.05) organic matter (OM) intake was recorded in rams reared on T1 diet. The total crude protein (CP) intake was in the following order: T5>T4>T3>T2>T1. Ram lambs receiving supplementary diets had higher (p<0.05) DM, OM, CP, neutral detergent fiber and acid detergent fiber digestibility compared with the control diet. The empty body weight and slaughter weight was highest (p<0.05) in rams receiving T3, T4, and T5 diets. The average daily gain and feed conversion efficiency was highest (p<0.05) in rams receiving the supplementary diets. The dressing percentage on the basis of hot carcass weight linearly increased with increasing levels of desmodium. Rams reared on supplementary diet had higher (p<0.05) rib eye area compared with the control diet. Conclusion: In conclusion, when banana leaf is used as a supplement to poor quality grass, better body weight gain was obtained when fed in combination with desmodium.

The Effects of SBAR Reports Education on Communication Clarity, Communication Skill and Report Confident for Nursing Students -Adult Nursing Clinical Practicum- (SBAR 보고교육이 간호대학생의 의사소통명확성, 의사소통능력 및 보고자신감에 미치는 효과 - 성인간호학 실습 -)

  • Lee, Oi Sun
    • Journal of Digital Convergence
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    • v.16 no.10
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    • pp.253-260
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    • 2018
  • The purpose of this study was to investigate the effect of SBAR(Situation-Background-Assessment-Recommendation) reports on communication clarity, communication skill and report confident for nursing students. This study used a one group pre-post test design. The subjects of this study were 44 nursing students from G city, and collected by self - report questionnaires from march 6 to april 20, 2018. Data were analyzed by frequencies, paried t-test using SPSS Win 23.0. After SBAR reports education, levels of nursing student's communication clarity(t=-8.12, p<.001), communication skill(t=-4.37, p<.001) and report confident(t=-7.67, p<.001) were significantly higher than levels before SBAR reports education. SBAR reports education improved the communication clarity communication skill and report confident. It is recommended to use a lot of SBAR in clinical practice.