• Preventive Nutrition and Food Science
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• 제12권4호
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• pp.267-272
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• 2007

The objective of this study was to investigate antioxidant activities of Panax ginseng extracted with various solvents including n-hexane, chloroform, EtOAC, n-butanol and water. Among the various ginseng extracts, ethyl acetate (EtOAC) extracts showed the most powerful scavenging activities against DPPH radicals. Among the other solvent extracts, the butanol extract seemed relatively more effective in scavenging activity, followed by chloroform, water and hexane extracts. Moreover, the highest reducing power and ferrous ion chelating activity were found in the EtOAC extract followed by other extracts of ginseng. EtOAC extracts, which exhibited the best antioxidant activities of all solvent extracts of ginseng, possessed higher concentrations of total phenolics (777.61 mg/100 g) than other extracts. These results suggest that EtOAC extracts of ginseng (P. ginseng C.A. Meyer) have the most effective antioxidant capacity compared to n-hexane, chloroform, n-butanol and water tested in this study, and has important applications for the pharmaceutical and food industries.

• Journal of the Korean Wood Science and Technology
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• 제48권6호
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• pp.769-779
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• 2020

This study investigates the selective fractionation of lignin with uniform structures and lower molecular weight. Lignin solubilization was first performed using a solution of acetic acid (AA) and hydrogen peroxide (HP) (4:1, (v/v)) to form peracetic acid (PAA), which is a strong oxidant. After the PAA-induced solubilization that occurred at 80℃, totally soluble lignin was extracted by ethyl acetate (EA) and divided into organic- and water-soluble fractions. The EA fraction was then fractionated by open-column using three solutions (chloroform-ethyl acetate, methanol, and water) sequentially. With an increase in the solvent polarity during the fractionation step, the molecular weight of the lignin-derived compounds in the fraction increased. Remarkably, some lignin fractions did not have aromatic structures. These fractions were identified as carboxylic acid-containing polymers like poly-carboxylates. These results conclude that the selective production of lignin-derived polymers with specific molecular weight and structural characteristics could be possible through open-column fractionation.

• 분석과학
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• 제6권5호
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• pp.453-462
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• 1993

본 연구는 열장 흐름 분획법을 이용하여 혼합 용매에서의 폴리스티렌의 머무름을 측정하고 용매의 조성이 시료의 머무름거동에 미치는 영향을 조사하였다. 시료는 분자량이 35,000, 110,000, 200,000, 470,000인 폴리스티렌을, 이동상은 테드라히드로퓨란(THF), 클로로포름(CHL), 시클로헥산(CH), 그리고 벤젠(BZ)의 단일용매 및 혼합용매를 사용하였다. 시료의 분자량과 혼합용매의 조성의 변화에 따라서 시료의 머무름비와 열확산계수를 측정하였으며, 그 값들을 시료와 용매의 물리화학적인 성질과 연관시켜 설명하였다. 즉, 시료의 머무름비는 이동상의 밀도의 영향을 크게 받으며 이동상의 밀도가 클수록 머무름비가 증가함을 알 수 있었다. 또한 이동상에 포함된 poor 용매의 함량이 증가할수록 열확산계수는 감소하였다.

• Natural Product Sciences
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• 제10권5호
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• pp.223-227
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• 2004

This study was carried out to investigate inhibitory effect of extracts from the root of Paeonia lactiflora on postprandial hyperglycemia. Organic solvent (hexane, ethyl acetate, butanol, aqueous) extracts from the crude drug were fractionated by high performance liquid chromatography. These fractions were examined to evaluate ${\alpha}-glucosidase$ (EC 3. 2. 1. 20) inhibition by microplate colorimetric assay. Among the fractions examined, the ethyl acetate fraction from the roots of Paeonia lactiflora showed potent inhibitory effects on ${\alpha}-glucosidase$. Therefore, further fractionation of the fraction was carried out to isolate the active principles. Finally, we isolated and Purified 1,2,3,4,6-penta-O-galloyl-beta-D-glucose (PGG) as a active principle by activity-guided fractionation. These results suggest that the extract from the root of Paeonia lactiflora can be used as a new nutraceutial for inhibition on postprandial hyperglycemia and PGG might be a candidate for developing an ${\alpha}-glucosidase$ inhibitor.

• 약학회지
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• 제46권5호
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• pp.352-357
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• 2002

The three crude drugs of the Kalopanax pictus (Araliaceae) roots (K), Pueraria thunbergiana (Leguminosae) flowers (P), and the Rhus verniciflua (Anacardiaceae) heartwood(R) used for anti-thirst drugs in Oriental herb medicine were extracted with MeOH, respectively, and the successive fractionation of the extract gave EtOAc extract. Certain amount ratios of the three extracts were also prepared to compare the antimutagenicity in Ames test. In N-methyl-N(-nitro-N-nitrosoguanidine (MNNG; 0.4 (g/plate)-induced test, the activities of complex mixture were observed between the highest antimutagenic activity of K extract and the lowest P extract. In aflatoxin (AFB$_1$)-induced test, the EtOAc complex (K : P : R=l : 1 : 3) labeled E-113 decreased the revertants of Salmonella typhimurium TA100 by 95％, which activity were highest among other extracts or complexes mixture used. Fractionation of organic solvent mostly increased the antimutagenicity. These trends were also observed in the antimutagenicity test of the mixture of each active component of kalopanaxsaponin A, tectorigenin and sulfuretin. These results supported that many kinds of anti-thirst herb medicine in the prescription could effectively prevent cancer disease.

• 생약학회지
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• 제49권3호
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• pp.278-284
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• 2018

Asimina triloba contains many kinds of annonaceous acetogenins known to have toxicity and/or activity. In this study, analysis of acetogenins was carried out on the extract and its solvent fractions of A. triloba leaves using a liquid chromatography mass spectroscopy(LC-MS). In the extract, the ethanolic extract was significantly higher acetogenin contents than hot water extract. In the analysis of solvent fraction from the ethanolic extract, n-hexane, ethylacetate, and aqueous fraction were contained 85.17~90.92%, 8.96~14.52% and 0.12~0.35% of total acetogenin in the extract. Based on these results, the analytical method is useful for comparing annonaceous acetogenins contents in plant samples. Moreover, when research or product development aiming at the physiological activity of acetogenin such as anticancer activity using A. triloba leaves is performed, a fractionation using a nonpolar organic solvents and alcohol extract may be useful methods. This method can also be used for the purpose of eliminating toxicity as well.

• 생약학회지
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• 제41권1호
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• pp.62-66
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• 2010

In result of analysis for the content determination of ursolic acid(UA) and oleanolic acid(OA) in extract of Eriobotryae folium using HPLC with UV detector, UA in chloroform extract of Moo-mok variety was showed highest content(2.7843 mg/g). And OA in ethyl acetate extract of Dae-bang variety was showed highest content(0.5898 mg/g). These result suggest that direct extraction using organic solvent(chloroform or ethyl acetate) was useful method for rapid quantitative analysis of UA and OA without preprocessing such as drying or fractionation.

• 한국미생물·생명공학회지
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• 제28권4호
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• pp.209-213
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• 2000

The cultured mycelia of Ganoderma lucidum were extracted and the extract was separated into six fractions by organic solvent fractionation. The antihepatotoxic activity of all the fractions was evaluated by measuring activities of glutamic pyruvic transaminase (GPT) and glutamic oxaloacetic transaminase (GOT). Among the fractions tested, the high-polarity fractions such as aqueous and n-butanol fractions significantly reduced activities of GPT and GOT in CCl4- and galactosamine-intoxicated rat primary hepatocytes. When intracellular synthetic activities were measured by pulsing the rate primary cultured hepatocytes with [3H]-uridine and [3H]-leucine, activities of DNA, RNA and protein. When direct toxicities of the fractions were measured against human hepatoma(SK-Hep-1), the non-polarity fractions such as n-butanol and ethyl acetate fractions showed potent direct cytotoxicities even at the concentration of 1 $\mu\textrm{g}$/ml. These data showed that Ganoderma lucidum has hepatoprotective and hepatotoxic recovery principles in its mycelia.

• 한국자원식물학회지
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• 제31권1호
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• pp.10-15
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• 2018

황칠나무 잎과 줄기를 분리하여 각각 methanol 추출하여, 계통분획상법에 따라 dichloromethane, ethyl acetate, butanol로 분획하였다. 분획물로 실시한 TLC에서 알칼로이드 성분으로 분리되는 성분을 확인할 수 있었다. 잎과 줄기 모두 ethyl acetate분획물은 $IC_{50}$ $30{\mu}g/m{\ell}$으로 높게 측정 되었으며, ethyl acetate, dichloromethane, butanol분획물 순으로 저해 활성이 높게 나타났다. Acetyl cholinesterase inhibition assay를 실시한 결과 황칠나무 잎과 줄기 모두 dichloromethane, ethyl acetate, butanol 분획물 순으로 저해 활성이 높은 것으로 나타났으며, 가장 높은 활성을 보인 황칠나무 줄기와 잎의 dichloromethane 분획물의 알칼로이드 함량이 상대적으로 높은 것으로 보아 알칼로이드 성분에 의한 것으로 유추 할 수 있으므로, 알칼로이드 성분의 동정 및 성분구조의 규명을 통하여 AChE저해 활성을 이용한 신경관련 질환에 도움을 줄 수 있는 천연물의 개발이 가능할 것으로 사료된다.

• 생명과학회지
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• 제23권3호
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• pp.361-367
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• 2013

Hizikia fusiformis을 80% EtOH 추출물을 용매의 극성에 따라 n-hexane, ethyl acetate, n-BuOH, Aqueous n-BuOH fraction으로 순차적으로 분리하였다. 분리된 4개의 분획물 중에 항산화 활성이 높은 n-BuOH 분획물을 reserve-phase column chromatography를 실시하여 분리된 3개의 BA, BB, BC 분획물에 대한 항산화 활성을 검토하였다. 가장 항산화능이 높은 BB 분획물은 모든 실험에서 농도의존적으로 활성이 증가하는데, 시료의 농도가 1.0 mg/ml일때 DPPH radical scavenging activity와 Reducing power activity는 $45{\pm}0.14%$, $1.34{\pm}0.23$로 가장 높게 나타난다. ${\beta}$-carotene-linoleic acid activity와 Hydrogen peroxide scavenging activity도 $76{\pm}0.12%$와 $82{\pm}0.06%$로 가장 높았으며, ABTS radical scavenging activity도 $65{\pm}0.17%$의 활성을 나타낸다. 이상의 연구 결과에서, 최종 분리된 BB분획물은 높은 항산화능을 가진 물질을 함유하고 있을 것으로 예상되며, 이는 앞으로 새로운 천연 기능성 식품으로서의 이용 가능성을 시사하고 있다.