• Journal of the Korea Fashion and Costume Design Association
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• v.4 no.1
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• pp.173-183
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• 2002

The orchid has a very elegant color and various forms in the nature or the ground for human life and the mother of life, The purpose of this study is to express some creative formative art by using the orchid as motive, and thereby, suggest the possibility of an artistic modern costume design by reviewing the texture of the materials. The results of this study can be summarized as follows; First, it was confirmed through this study that the orchid with diverse forms and colors can be used as design motive in almost infinitely diverse ways, and that all the artificial forms or structures contain the elements of natural beauty. Second, the luxurious and rich sense of orchid‘s color can be maximized by using the complementary contrast effect of the dyeing technique and the color gradation effect through harmony among similar colors. Third, a high value-added costume can be created to meet modern men’s aesthetic desire by using such embroidery techniques as crochet, weaving, dyeing, beads embroidery and art flower. Fourth, different effects of texture can be rendered by using different materials of various textures and characteristics. In addition, the possibility of creative expression for costume as formative art can be enhanced by expanding the expressions of the materials.

• Mycobiology
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• v.31 no.3
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• pp.151-156
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• 2003

Two fungal isolates obtained from roots of Cymbidium goeriingii in Jeju island were confirmed to be symbiotic with orchid plantlets, and were compared with other orchid mycorrhizal(OM) fungi previously isolated. The two isolates differed in their peloton structures formed in the roots of Cymbidium kanran hybrid 'Chungsu' and in responses of orchid plant. These two isolates differed from the additionally tested OM fungi in some features, and from root damaging species of Rhizoctonia and Fusarium as based on cluster analysis after PCR-RAPD with the primers, Bioneer-28 and OPO-2. With this simple and fast technique, it was possible to distinguish OM fungi from the plant root pathogenic fungi based on calculation of their polymorphic bands. This technique can therefore be helpful to distinguish the OM fungi from the root pathogens. Particularly, the new isolates are considered as new resource of symbiotic fungi for horticultural industries.

• The Korean Journal of Mycology
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• v.43 no.2
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• pp.129-132
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• 2015

In this study, orchid mycorrhizal fungi (OMF) were isolated from four terrestrial orchids on Mt. Hambeak, Platnathera chlorantha, Platnathera mandarinorum, Cephalanthera falcate, and Cephalanthera longibracteata. OMF were identified using morphological and sequences analysis of fungal internal transcribed spacer (ITS) regions by specific primer of basidiomycetous orchid mycorrhizas; ITS1-OF and ITS4-OF. Four species of orchid mycorrhizal fungi were identified as Ceratobasidium sp, Epulorhiza anaticula, Tulasnella calospora and Tulasnella sp.

• Korean Journal of Agricultural Science
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• v.50 no.2
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• pp.193-206
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• 2023

Orchidaceae species account for one-tenth of all angiosperms including more than 30,000 species having significant ecological, evolutionary, and economic importance. Despite Orchidaceae being one of the largest families among flowering plants, crucial cytogenetic information for studying species diversification, inferring phylogenetic relationships, and designing efficient breeding strategies is lacking, except for 10% or less of orchid species cases involving mostly chromosome number or karyotype analysis. Also, only approximately 1.5% of the identified orchid species from less than a hundred genera have genome size data that provide crucial information for breeders and molecular geneticists. Various molecular cytogenetic techniques, such as fluorescence in situ hybridization (FISH) and genomic in situ hybridization (GISH), have been developed for determining ploidy levels, analyzing karyotypes, and evaluating hybridity, in several ornamental crops including orchids. The estimation of genome size and the determination of nuclear DNA content using flow cytometry have also been employed in some Orchidaceae subfamilies. These different techniques have played an important role in supplementing beneficial knowledge for effective plant breeding programs and other related plant research. This review focused on orchid breeding summarizes the status of current cytogenetic tools in terms of background, advancements, different techniques, significant findings, and research challenges. Principal roles and applications of cytogenetics in orchid breeding as well as different ploidy level determination methods crucial for breeding are also discussed.

• Korean Journal of Breeding Science
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• v.41 no.4
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• pp.510-514
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• 2009

Cymbidium is one of the largest genus in the orchid family and a number of hybrids have been bred in the world. During mass-propagating the Cymbidium "Dongyang" using the meristem culture technology, a useful leaf mutant was selected from the protocom like bodies. The new Cymbidium variety by in vitro mutangesis from "Dongyang" was named as 'Daegook' in 1998. Compared to Dongyang, "Daegook" mutant has white or yellow stripes along the margin of leaves and flowers. The plant length of "Daegook" was shorter than "Dongyang" and the mean length and width of leaf in "Daegook" was 40 cm and 1.6 cm, respectively. The new variety, "Daegook", is expected to be a popular Cymbidium variety among consumer as a ornamental orchid mutant for pot culture by its fine and unique stripes and growth characters.

• The Plant Pathology Journal
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• v.15 no.1
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• pp.34-37
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• 1999

Cymbidium mosaic virus (CyMV) was purified from CyMV infected orchid plant leaves by Sephacryl S-500 HR column chromatography. Partial purification was done by solubilization with Triton X-100 (alkylphenoxypolyethoxy ethanol) and precipitation with polyethylene glycol (PEG 6,000) followed by ultracentrifugation on 30% sucrose cushion. Based on the spectrophotometric analysis, 33 mg of CyMV could be obtained form 100 g of CyMV-infected orchid plant leaves. The purified CyMV represented one distinct homogeneous band by SDS-PAGE, and electron microscopy revealed that it was highly homogeneous and not fragmented. Bioassay demonstrated that the purified CyMV had a normal infectivity to Chenopodium amaranticolor and orchid plants. Based on these results, the purification method in this work could be served as an improved method for the purification of CyMV and similar viruses with good yield, high purity and native integrity.

• The Korean Journal of Mycology
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• v.41 no.1
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• pp.9-13
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• 2013

In this study, roots of Epipactis thunbergii were collected from Chujado on the north of Jeju-do. Six fungal isolates were isolated from surface-sterilized roots of the orchid and classified with groups based on morphological characteristics. Fungal DNA was extracted from each isolate and amplified ITS region using ITS1-OF/ITS4-OF primer pair. Three species of orchid mycorrhizal fungi were identified as Tulasnella calospora, Tulasnella sp. and Sebacina sp. based on molecular and morphological characteristics.

• Journal of the Korea Institute of Building Construction
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• v.4 no.1
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• pp.89-96
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• 2004

Porous concrete is used of various parts by advantage of porous. Example of growing of plant is possible, and dwelling of creature, and filter functions of various contaminant, and decrease of noise, and so on. This research is for porous concretes that were used by four aggregate rubble, refreshing aggregate, expanded clay, orchid stone. This research estimate that physical and mechanical characteristics of fresh concrete and hardened concrete. The purpose of this research is to make environment-friendly porous concrete. This research's conclusion is as following : 1. Porous Concrete's slump was measured 12~14cm with rubble, 12~16cm with refreshing aggregate, 11~13cm with expanded clay, 11~13cm with orchid stone. Weight of aggregate was bigger, slump price appeared by bigger thing. Because placed Porous Concrete is low viscosity and small resistance between aggregate, it estimated that have high workability. 2. Porous Concrete's unit weight was measured 1.71~1.75t/$\textrm{m}^3$ with rubble, 1.58~1.62t/$\textrm{m}^3$ with refreshing aggregate, 1.19~1.20t/$\textrm{m}^3$ with expanded clay, 0.98~1.06t/$\textrm{m}^3$ with orchid stone. Showed aspect such as weight of aggregate. 3. Porous Concrete's compressive strength was measured 76~102kgf/$\textrm{cm}^2$ with rubble, 51~60kgf/$\textrm{cm}^2$ with refreshing aggregate, 30~40kgf/$\textrm{cm}^2$ with expanded clay, 13~16kgf/$\textrm{cm}^2$ with orchid stone. 4. Tendency of tensile strength and bending strength showed generally similarly with compressive strength, but showed low value fewer than 15kgf/$\textrm{cm}^2$ Therefore, wire mesh, reinforcing rod, such as establishment of frame is considered to need in reinforcement about tensility or flexures in case receive tensility or produce product of thin absence form. It concludes by speculating on the consequences of extrapolating the results of study to remodelling the office building being already existence.

• The Korean Journal of Mycology
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• v.38 no.1
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• pp.1-4
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• 2010

Orchid mycorrhizal fungi(OMF) were examined in roots of the two threatened orchid species, Cypripedium japonicum and C. macranthum. The morphological characteristics of mycorrhizal colonization in the roots of two orchid species were observed. OMF colonized in the roots of two species were identified using molecular analysis. DNA from the root was extracted and amplified internal transcribed spacer(ITS) region using basidiomycete ITS primers, ITS1-OF and ITS4-OF. Four species belonging to Tulasnellaceae in roots of C. japonicum and two species of Tulasnellaceae and one basidiomycetous species was found in roots of C. macranthum.

• The Korean Journal of Mycology
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• v.33 no.2
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• pp.92-94
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• 2005

A orchid rotting fungus was isolated and identified. The isolate was consistent with the genus Fusarium in morphological and cultural characteristics. The partial 18S rRNA sequence of the isolate showed high similarity with anamorph or telemorph of Fusarium and other Fusarium species. In phylogenetic analysis, the isolates was poorly related to other Fusarium species. The isolate closely related to Fusarium sp. LP-A2/3.