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http://dx.doi.org/10.4489/KJM.2010.38.1.001

Characteristic of Orchid Mycorrhizal Fungi from Roots of Cypripedium japonicum and C. macranthum  

Sim, Mi-Yeong (Department of Biology Education, Korea National University of Education)
Youm, Jae-Young (Department of Biology Education, Korea National University of Education)
Chung, Jae-Min (Department of Forest Resources Conservation, Korea National Arboretum)
Lee, Byung-Chun (Department of Forest Resources Conservation, Korea National Arboretum)
Koo, Chang-Duck (Department of Forestry, Chungbuk National University)
Eom, Ahn-Heum (Department of Biology Education, Korea National University of Education)
Publication Information
The Korean Journal of Mycology / v.38, no.1, 2010 , pp. 1-4 More about this Journal
Abstract
Orchid mycorrhizal fungi(OMF) were examined in roots of the two threatened orchid species, Cypripedium japonicum and C. macranthum. The morphological characteristics of mycorrhizal colonization in the roots of two orchid species were observed. OMF colonized in the roots of two species were identified using molecular analysis. DNA from the root was extracted and amplified internal transcribed spacer(ITS) region using basidiomycete ITS primers, ITS1-OF and ITS4-OF. Four species belonging to Tulasnellaceae in roots of C. japonicum and two species of Tulasnellaceae and one basidiomycetous species was found in roots of C. macranthum.
Keywords
Cypripedium japonicum; Cypripedium macranthum; OMF; Orchid mycorrhizal fungi; Peloton; Tulasnellaceae;
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