The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Identification of Orchid Mycorrhizal Fungi Isolated from Epipactis thunbergii in Korea

닭의난초(Epipactis thunbergii)에 공생하는 난 균근균의 분리 및 동정

  • Han, Han-Kyeol (Department of Biology, Korea National University of Education) ;
  • Chung, Jae-Min (Department of Plant Resources Conservation, Korea National Arboretum) ;
  • Cho, Yong-Chan (Department of Plant Resources Conservation, Korea National Arboretum) ;
  • Kim, Dae-Shin (Research Institute for Hallasan) ;
  • Eom, Ahn-Heum (Department of Biology, Korea National University of Education)
  • 한한결 (한국교원대학교 생물교육과) ;
  • 정재민 (국립수목원 산림자원보존과) ;
  • 조용찬 (국립수목원 산림자원보존과) ;
  • 김대신 (제주특별자치도 한라산 연구소) ;
  • 엄안흠 (한국교원대학교 생물교육과)
  • Received : 2013.03.05
  • Accepted : 2013.03.20
  • Published : 2013.03.31
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Abstract

In this study, roots of Epipactis thunbergii were collected from Chujado on the north of Jeju-do. Six fungal isolates were isolated from surface-sterilized roots of the orchid and classified with groups based on morphological characteristics. Fungal DNA was extracted from each isolate and amplified ITS region using ITS1-OF/ITS4-OF primer pair. Three species of orchid mycorrhizal fungi were identified as Tulasnella calospora, Tulasnella sp. and Sebacina sp. based on molecular and morphological characteristics.

제주도의 북쪽에 위치한 추자도에서 닭의난초 뿌리를 채취하였고, 표면 살균한 뿌리에서 6개의 균주를 분리하였다. 각 균주는 형태적 특징을 기초로 그룹을 나누었고, 난 균근균에 특이적인 프라이머인 ITS1-OF/ITS4-OF를 이용하여 균주의 rDNA의 ITS지역을 증폭하였다. 형태적 특징과 분자적 분석을 통하여 6개의 균주를 Sebacina sp., Tulasnella calospora, Tulasnella sp. 총 3종의 난 균근균으로 동정하였다.

Keywords

References

  1. Batty, A. L., Brundrett, M. C., Dixon, K. W. and Sivasithamparam, K. 2006. New methods to improve symbiotic propagation of terrestrial orchid seedlings from axenic culture to soil. Aust. J. Bot. 54:367-374. https://doi.org/10.1071/BT04023
  2. Bidartondo, M. I., Burghardt, B., Gebauer, G., Bruns, T. D. and Read, D. J. 2004. Changing partners in the dark: isotopic and molecular evidence of ectomycorrhizal liaisons between forest orchids and trees. Proc. Roy. Soc. Lond. B Biol. Sci. 271:1799-1806. https://doi.org/10.1098/rspb.2004.2807
  3. Chung, M. Y. and Chung, M. G. 2007. Extremely low levels of genetic diversity in the terrestrial orchid Epipactis thunbergii (Orchidaceae) in South Korea: implications for conservation. Bot. J. Linn. Soc. 155:161-167. https://doi.org/10.1111/j.1095-8339.2007.00698.x
  4. Declerck, S., Strullu, D. G. and Fortin, A. 2005. In vitro culture of mycorrhizas, 4th edition, pp. 291-312. Springer, France.
  5. Lee, N. S. 2011. Illustrated flora of Korean Orchids. Ewha Womans University Press, Seoul. (in Korean).
  6. Lee, S. S., Oh, C. H., Paek, K. Y. and Lee, T. S. 1998. Isolations of the orchid mycorrhizal fungi from the roots of the Korean native orchids and inoculations of the isolates to four different orchids. Kor. J. Plant Pathol. 14:536-542. (in Korean).
  7. Peterson, R. L., Uetake, Y. and Zelmer, C. 1998. Fungal symbioses with orchid protocorms. Symbiosis 25:29-55.
  8. Pope, E. J. and Carter, D. A. 2001. Phylogenetic placement and host specificity of mycorrhizal isolates belonging to AG-6 and AG-12 in the Rhizoctonia solani species complex. Mycologia93:712-719. https://doi.org/10.2307/3761825
  9. Rasmussen, H. N. and Whigham, D. F. 1993. Seed ecology of dust seeds in situ: a new study technique and its application in terrestrial orchids. Am. J. Bot. 80:1374-1378. https://doi.org/10.2307/2445665
  10. Selosse, M. A., Bauer, R. and Moyersoen, B. 2002. Basal Hymenomycetes belonging to the Sebacinaceae are ectomycorrhizal on temperate deciduous trees. New Phytol.155:183-195. https://doi.org/10.1046/j.1469-8137.2002.00442.x
  11. Sim, M. Y., Youm, J. Y., Chung, J. M., Lee, B. C., Koo, C. D. and Eom, A. H. 2010. Characteristic of orchid mycorrhizal fungi from roots of Cypripedium japonicum and C. macranthum. Kor. J. Mycol. 38:1-4. (in Korean). https://doi.org/10.4489/KJM.2010.38.1.001
  12. Smith, S. E. and Read, D. J. 2008. Mycorrhizal symbiosis. Academic Press, New York.
  13. Sneh, B., Burpee, L. and Ogoshi, A. 1991. Identification of Rhizoctonia species. APS Press, St. Paul.
  14. Taylor, D. L., Bruns, T. D., Szaro, T. M. and Hodges, S. A. 2003. Divergence in mycorrhizal specialization within Hexalectris spicata(Orchidaceae), a nonphotosynthetic desert orchid. Am. J. Bot. 90:1168-1179. https://doi.org/10.3732/ajb.90.8.1168
  15. Taylor, D. L. and McCormick, M. K. 2008. Internal transcribed spacer primers and sequences for improved characterization of basidiomycetous orchid mycorrhizas. New Phytol. 177:1020-1033. https://doi.org/10.1111/j.1469-8137.2007.02320.x
  16. Youm, J. Y., Chung, J. M., Lee, B. C. and Eom, A. H. 2011. Molecular identification of orchid mycorrhizal fungi of native orchids in Ulleung Island. Kor. J. Mycol. 39:7-10. (in Korean). https://doi.org/10.4489/KJM.2011.39.1.0007
  17. Youm, J. Y., Han, H. K., Chung, J. M., Cho, Y. C., Lee, B. C. and Eom, A. H. 2012. Identification of orchid mycorrhizal fungi isolated from five species of terrestrial orchids in Korea. Kor. J. Mycol. 40:132-135. (in Korean). https://doi.org/10.4489/KJM.2012.40.3.132
  18. Yuan, L., Z. I. Yang, S. Y. Li, H. Hu and J. L. Huang. 2010. Mycorrhizal specificity, preference and plasticity of six slipper orchids from South Western China. Mycorrhiza 20:559-568. https://doi.org/10.1007/s00572-010-0307-5
  19. Warcup, J. H. and Talbot, P. H. B. 1967. Perfect states of Rhizoctoniasassociated with orchids. New Phytol. 66:631-641. https://doi.org/10.1111/j.1469-8137.1967.tb05434.x

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