Browse > Article
http://dx.doi.org/10.4489/KJM.2015.43.2.129

Identification of Orchid Mycorrhizal Fungi Isolated from Terrestrial Orchids in Mt. Hambaek, Korea  

Lee, Bong-Hyung (Department of Biology Education, Korea National University of Education)
Han, Han-Kyeol (Department of Biology Education, Korea National University of Education)
Eom, Ahn-Heum (Department of Biology Education, Korea National University of Education)
Publication Information
The Korean Journal of Mycology / v.43, no.2, 2015 , pp. 129-132 More about this Journal
Abstract
In this study, orchid mycorrhizal fungi (OMF) were isolated from four terrestrial orchids on Mt. Hambeak, Platnathera chlorantha, Platnathera mandarinorum, Cephalanthera falcate, and Cephalanthera longibracteata. OMF were identified using morphological and sequences analysis of fungal internal transcribed spacer (ITS) regions by specific primer of basidiomycetous orchid mycorrhizas; ITS1-OF and ITS4-OF. Four species of orchid mycorrhizal fungi were identified as Ceratobasidium sp, Epulorhiza anaticula, Tulasnella calospora and Tulasnella sp.
Keywords
Ceratobasidium; Epulorhiza anaticula; Orchid mycorrhizal fungi; Tulasnella calospora;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Swarts ND, Dixon KW. Terrestrial orchid conservation in the age of extinction. Ann Bot 2009;104:543-56.   DOI   ScienceOn
2 Rasmussen HN. Recent developments in the study of orchid mycorrhiza. Plant Soil 2002;244:149-63.   DOI   ScienceOn
3 Sneh B, Burpee L, Ogoshi A. Identification of Rhizoctonia species. St. Paul (MN): APS Press; 1991.
4 Moore RT. The genera of Rhizoctonia-like fungi: Ascorhizoctonia, Ceratorhiza gen. nov., Epulorhiza gen. nov., Moniliopsis, and Rhizoctonia. Mycotaxon 1987;29:91-9.
5 Dearnaley JD. Further advances in orchid mycorrhizal research. Mycorrhiza 2007;17:475-86.   DOI
6 Cameron DD, Leake JR, Read DJ. Mutualistic mycorrhiza in orchids: evidence from plant-fungus carbon and nitrogen transfers in the green-leaved terrestrial orchid Goodyera repens. New Phytol 2006;171:405-16.   DOI   ScienceOn
7 Leake JR. The biology of myco-heterotrophic ('saprophytic') plants. New Phytol 1994;127:171-216.   DOI   ScienceOn
8 Youm JY, Han HK, Chung JM, Cho YC, Lee BC, Eom AH. Identification of orchid mycorrhizal fungi isolated from five species of terrestrial orchids in Korea. Kor J Mycol 2012;40:132-5.   DOI   ScienceOn
9 Han HK, Chung JM, Cho YC, Kim DS, Eom AH. Identification of orchid mycorrhizal fungi isolated from Epipactis thunbergii in Korea. Kor J Mycol 2013;41:9-13.   DOI   ScienceOn
10 Lee SS, Hong SW, Lee JK, Kim U, Park SY, Paek KY. Isolation of the orchid mycorrhizal fungi from the Korean native orchid in Cheju. Mycobiology 2000;28:97-102.
11 Richardson KA, Currah RS, Hambleton S. Basidiomycetous endophytes from the roots of neotropical epiphytic Orchidaceae. Lindleyana 1993;8:127-37.
12 Gardes M, Bruns TD. ITS primers with enhanced specificity for basidiomycetes-application to the identification of mycorrhizae and rusts. Mol Ecol 1993;2:113-8.   DOI   ScienceOn
13 Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011;28:2731-9.   DOI   ScienceOn
14 Filipello Marchisio V, Berta G, Fontana A, Marzetti Mannina F. Endophytes of wild orchids native to Italy: their morphology, caryology, ultrastructure and cytochemical characterization. New Phytol 1985;100:623-41.   DOI   ScienceOn
15 Pope EJ, Carter DA. Phylogenetic placement and host specificity of mycorrhizal isolates belonging to AG-6 and AG-12 in the Rhizoctonia solani species complex. Mycologia 2001:712-9.   DOI
16 Warcup JH. The mycorrhizal relationships of Australian orchids. New Phytol 1981;87:371-81.   DOI   ScienceOn
17 Currah RS, Smreciu EA, Hambleton S. Mycorrhizae and mycorrhizal fungi of boreal species of Platanthera and Coeloglossum (Orchidaceae). Can J Bot 1990;68:1171-81.   DOI
18 Nontachaiyapoom S, Sasirat S, Manoch L. Symbiotic seed germination of Grammatophyllum speciosum Blume and Dendrobium draconis Rchb. f., native orchids of Thailand. Sci Hortic 2011;130:303-8.   DOI   ScienceOn
19 Lee SS, You JH. Identification of the orchid mycorrhizal fungi isolated from the roots of Korean native orchid. Mycobiology 2000;28:17-26.
20 Hadley G. Non-specificity of symbotic infection in orchid mycorrhiza. New Phytol 1970;69:1015-23.   DOI   ScienceOn
21 Yuan L, Yang ZL, Li SY, Hu H, Huang JL. Mycorrhizal specificity, preference, and plasticity of six slipper orchids from South Western China. Mycorrhiza 2010;20:559-68.   DOI
22 Warcup J. Symbiotic germination of some Australian terrestrial orchids. New Phytol 1973;72:387-92.   DOI   ScienceOn
23 Zettler LW, Hofer CJ. Propagation of the little club-spur orchid (Platanthera clavellata) by symbiotic seed germination and its ecological implications. Environ Exp Bot 1998;39:189-95.   DOI   ScienceOn
24 Matsuda Y, Amiya A, Ito S. Colonization patterns of mycorrhizal fungi associated with two rare orchids, Cephalanthera falcata and C. erecta. Ecol Res 2009;24:1023-31.   DOI