• Journal of Applied Biological Chemistry
• /
• v.65 no.3
• /
• pp.129-142
• /
• 2022

Ginseng sprouts, which can be eaten from leaves to roots, has the advantage of not having to use pesticides without being affected by the season by using smart farms. The optimal cultivation timing of sprout ginseng was checked and the nutritional content and antioxidant activity were compared and analyzed. The values of total fatty acids and total minerals were no significant changes during the growth periods. The contents of total amino acids were slightly decreased to 45 days and after increased to 65 days. When the growth period was 65 days, arginine had the highest content of 3309.11 mg/100 g. The total phenolic contents were high at 3.73 GAE mg/g on the 45 days, and the total flavonoid contents were also the highest at 9.04 RE mg/g on the 45 days. The contents of total ginsenoside was not noticeable for the growth periods (29.83 on 25 days→32.77 on 45 days→26.02 mg/g on 65 days). The ginsenoside Rg2 (0.62 mg/g), Re (8.69 mg/g), Rb1 (4.75 mg/g) and Rd (3.47 mg/g) had highest contents on 45 days during growth. The values of phenolic acids and flavonols were gradually increased to 45 days (338.6 and 1277.14 ㎍/g) and then decreased to 65 days. The major compounds of phenolic acids and flavonols were confirmed to benzoic acid (99.03-142.33 ㎍/g) and epigallocatechin (416.03-554.64 ㎍/g), respectively. The values of 2,2-diphenyl-1-picrylhydrazyl (44.27%), 2,4,6-azino-bis (3-ethylbenzothiazoline-6-sulphnoic acid) diammonium salt (75.16%), and hydroxyl (63.29%) radical scavenging activities and ferric reducing/antioxidant power (1.573) showed the highest activity on the 45 days as well as results of total phenolic and total flavonoid contents.

• Journal of Mushroom
• /
• v.20 no.3
• /
• pp.113-118
• /
• 2022

This study was conducted to diversify the cultivation of mushroom items and develop a competitive variety of Hypsizigus marmoreus. We focused on developing Hypsizigus marmoreus with lower bitterness, likable shape, and high yield. We have collected and tested characteristics of genetic resources from domestic and abroad since 2019. Breeding (2019), characterization (2020), productivity test (2021), and farm demonstration test (2021) have been sequentially conducted. We bred the new variety 'Yunseul' with unique traits. The optimal temperature for mycelial and fruit body growth were 22-25℃ and 15-18℃, respectively. It was similar to the control variety (Mangadak-2Ho) in the pileus form (hemispherical shape) and the cultivation period. However, it was thinner and longer than the control variety with the pileus diameter, thickness, stipe diameter, and length being 19.9 mm, 7.0 mm, 9.4 mm, and 86.3 mm, respectively. The effective number of fruit bodies was 47.8 in bottle cultivation, which was more than that of the control variety. The yield was 197.4 g/bottle (1,100 cc), which was 30% higher than the yield of the control variety, 151.9 g/bottle (1,100 cc). The parent and control varieties were also incubated alongside the new variety. The somatic incompatibility line was distinct. The band pattern in the mycelial DNA PCR reaction was different from that of the parent and control varieties, confirming the hybrid species.

• Journal of Bio-Environment Control
• /
• v.31 no.4
• /
• pp.416-422
• /
• 2022

This study was conducted to investigate the effect of each light intensity and photoperiod combination on the growth and glucosinolates (GSLs) content of three species of Brassicaceae plants under the same daily light integral (DLI) conditions. Seeds of leaf mustard (Brassica juncea (L.) Czern.), red mustard(Brassica juncea L.) and kale (Brassica oleracea L. var. acephala (DC.) Alef.) were sown in a rockwool cubes and grown for three weeks. DLI was set to 10 mol·m^-2·d^-1 and treated with 10h-280, 14h-200, 18h-155, 22h-127 µmol·m^-2·s^-1 for three weeks. As a result at 14h-200 µmol·m^-2·s^-1 treatment, shoot fresh/dry weight, the number of leaves, and leaf area were increased in leaf mustard and kale but there was no significant difference in other treatments. In the total GSLs content, the treatment of 14h-200 µmol·m^-2·s^-1 increased significantly 139.95, 135.87, 154.03% compared to 10h-280, 18h-155, 22h-127 µmol·m^-2·s^-1 treatment in red mustard, and 14h-200 µmol·m^-2·s^-1 treatment increased significantly 132.96, 132.96, 134.03% compared to other treatments in kale. In red mustard, the treatment of 18h-155 µmol·m^-2·s^-1 showed an increase in shoot fresh/dry weight and the total GSLs contents than other photoperiods and 14h-200 µmol·m^-2·s^-1 treatment, the number of leaves significantly 15.62, 12.12, and 32.14% higher than other photoperiods. Since the DLI response is different depending on species even for similar Brassicaceae crops, it is necessary to get more detailed results by conducting optical light quality studies and deriving optimal DLI conditions to achieve minimum power consumption and maximum efficiency.

• Journal of Wetlands Research
• /
• v.24 no.2
• /
• pp.83-92
• /
• 2022

The amount of the plastic waste has been increasing according to global demand for plastic. Microplastics are the most hazardous among all plastic pollutants due to their toxicity and unknown physicochemical properties. This study investigates the optimal methodology that can be applied to sewage samples for detecting microplastics before discussing reducing microplastics in MWTPs. In this study, the effect of different pretreatment methods while detecting microplastic analysis of MWTP influent samples was investigated; the samples were collected from the J sewage treatment plant. There are many pretreatment methods but two of them are widely used: Fenton digestion and hydrogen peroxide oxidation. Although there are many pretreatment methods that can be applied to investigate microplastics, the most widely used methods for sewage treatment plant samples are Fenton digestion and H₂O₂ oxidation. For each pretreatment method, there were factors that could cause an error in the measurement. To overcome this, in the case of the Fenton digestion pretreatment, it is recommended to proceed with the analysis by filtration instead of the density separation method. In the case of the H₂O₂ oxidation method, the process of washing with distilled water after the reaction is recommended. As a result of the analysis, the concentration of microplastics was measured to be 2.75ea/L for the sample using the H₂O₂ oxidation method and 3.2ea/L for the sample using the Fenton oxidation method, and most of them were present in the form of fibers. In addition, it is difficult to guarantee the reliability of measurement results from quantitative analysis performed via microscope with eyes. A calibration curve was created for prove the reliability. A total of three calibration curves were drawn, and as a result of analysis of the calibration curves, all R² values were more than 0.9. This ensures high reliability for quantitative analysis. The qualitative analysis could determine the series of microplastics flowing into the MWTP, but could not confirm the chemical composition of each microplastic. This study can be used to confirm the chemical composition of microplastics introduced into MWTP in the future research.

• Economic and Environmental Geology
• /
• v.55 no.5
• /
• pp.429-438
• /
• 2022

This study evaluated the effect of the degree of weathering on the particle size distribution and the amount of fine particles generated in the aggregate production process during the crushing of igneous rock. Rock samples were collected from three areas with differences in strength from the Schmith hammer measurement at the aggregate quarry in Geochang, Gyeongsangbuk-do. After crushing with a jaw crusher under the same conditions in laboratory, particle size analysis, mineral analysis, chemical analysis, and weathering index were calculated. The Schmidt hammer measurements were 56, 28, and <10, and the CIA and CIW values of weathering index were also different, so the rock samples were classified into hard rock, soft rock, and weathered rock according to the weathering degree. It shows a smaller particle size distribution toward weathered rocks under the microscope, and the proportion of altered clay minerals such as sericite increased. The composition of feldspar and quartz was high for hard rock, and the ratio of muscovite and kaolinite was low. As a result of the crushing of the jaw crusher, hard rock produced a lot of coarse crushed material (13.2mm), while soft rock and weathered rock produced fine crushed material (4.75mm). The former showed the characteristics of the beta distribution curve, and the latter showed the bimodal distribution curve. The production of fine rock particles (based on 0.71mm of sieve, wt. %) increased to 13%<21%<22% in hard rock, soft rock, and weathered rock, and the greater the degree of weathering, the more fine rock particles were generated. The fine particles are recovered by the operation of the sand unit in the wet aggregate production process. Therefore, in order to minimize the amount of sludge generated in the aggregate production process, it was judged that a study on the optimal operation of cyclones could be necessary.

• Economic and Environmental Geology
• /
• v.55 no.5
• /
• pp.477-495
• /
• 2022

This study examined the sustainability of CO₂-rich water by analyzing the water level and water quality change pattern with the amount of its use in Angseong area, Chungju. The origin and supply of CO₂ component were discussed in consideration of ⁸⁷Sr/⁸⁶Sr ratio, occurrence of CO₂-rich fluid inclusions in nearby W-Mo deposits and other surrounding geological characteristics. According to the data from 1986 to 2017, the depth of the water level of CO₂-rich water was significantly lowered in the late period (2009-2015) than in the early period (1986-1992) of the development of hot spa wells, and the optimal yields for pumping tests also showed a tendency to gradual decrease. Concentrations of CO₂ component also decreased continuously in the later stages compared to the early stages of development, but it has been stable since 2012. It is inferred that the geological environment related to forming W-Mo quartz vein deposits (0.5×1.5×several km) around the study area are largely involved in the origin and supply of CO₂ component, and the supply of CO₂ component is not infinitely supplied from deep current magma activity. Rather, since it is finitely supplied from a restricted subsurface region formed in the past geological period, it is necessary to efficiently control its use in order to maintain the sustainability of CO₂-rich water in the study area.

• Korean Journal of Environment and Ecology
• /
• v.12 no.1
• /
• pp.58-77
• /
• 1998

Four species(25%) of Viperidae(Agkistrodon brevicaudus, Agkistrodon ussuriensis, Agkistrodon saxatilis) and Cloubridae(Rhabdophis tigrenus tigrenus) were Korean poisonous snake. Copulation season of these species was from July to August. Reproduction mode of genus Agkistrodon species was ovoviviparous but Rhabdophis tigrinus tigrinus was the other pattern of oviparous. Optimal movement temperature range was from 20$\circ $C to 29$\circ $C(March~September). Wjen atmosphere temperature was below 10$\circ $C, at that time they hibernate at the ground, rock bottom, stone wall and embankment around the end of a field. The venom of these snakes consist mainly Hematoxin, Cytolysin, Neurotoxin and Cardiotoxin of poisonous liquids. These material injection to animal cause systemic syndrome such as Dizziness(25.7%), Vomitting(23.1%), Fever(22%), Visual trouble(18%), Headace(17.7%), Dyspnoea(17.6%) and bring about other local syndrome such as Discoloration(54.2%), Bleeding(20.2%), Bullae(10.7%) and Skin ulcer(!0.8%). The annual distribution was appeared to decrease 1972 after 1992 and average snakebiting patients was 25.6 per year, but practically total estimated snakebiting was 2,700 per year. The seasonal distribution was most frequent in August(25%), and mortality was 1.8%(26 per 1,430). The sex ratio was 2:1 and according to age distribution, it was most prevalent at one's fifties(19%). The most frequent place where the accident happened was the field(48.2%) and most predilection site of the body for victim were hand(47.8%) and foot(39.5%), Commonly bite snake were Agkistrodon ussuriensis(27.1%), Agkistrodon brevicaudus(22.6%) and Agkistrodon saxatilis(9.6%) but 40.7% of species could not be identified. Treatment of antivenin patient was 75.9% (1,068/1,407).

• Korean Journal of Poultry Science
• /
• v.50 no.1
• /
• pp.1-13
• /
• 2023

The study was carried out to investigate the effects of dietary combined supplementation of antioxidants as catechin and vitamin C on growth performance, meat quality, blood profiles and stress responses of broilers exposed to high temperature. For this experiment, a total of 360 21-day-old male Ross 308 broilers were used. Treatments were assigned with 6 replicates per treatment and 10 birds per replicate in a 2 × 3 factorial design with vitamin C (0, 250 mg/kg) and catechin (0, 600, 1,200 mg/kg). The heat stress environment was maintained at temperature 32±1℃ and relative humidity 60±5% for 24 hours until the end of the experiment. The supplemented antioxidants had no significant difference in weight gain, feed intake and feed conversion ratio (P>0.05). The content of total cholesterol in blood had no interaction, but decrease (P<0.01) in the supplemented catechin group. Also, the supplementation with catechin showed increase in the SOD activity of blood, and lower corticosterone and IgM levels of broilers. The contents of HSP70 and MDA in liver decrease (P<0.05) with the supplementation of antioxidants, and HSP70 showed an interaction between groups. DPPH radical scavenging ability in breast meat increased (P<0.01) in catechin, but meat quality did not show difference according to treatments. Respiratory rate decreased (P<0.05) in catechin, but no interaction with vitamin C. In conclusion, the combination of vitamin C and catechin can alleviate stress under high temperature, such as HSP70 and MDA, but further study on the optimal supplemental level is needed.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.67 no.4
• /
• pp.199-210
• /
• 2022

This study aimed to develop a cropping system to use limited crop-land with optimum efficiency, while considering management from farmers. To establish the cropping system involving a two-year rotation of three crops, three types of cropping system were evaluated in Suwon (Seogcheon series) and Anseong (Geumcheon series) in the middle plain area using six crops from 2018 to 2019: maize-perilla-onion, potato-sesame-garlic, and maize-sesame-onion. The crop productivity and income of the cropping systems involving food-, oilseed-, and horticultural crops were analyzed, and the optimal cropping system was reviewed. The total yield of each crop was as follows: maize 1,281 kg, potato 4,837 kg, perilla 125 kg, sesame 120 kg, onion 6,503 kg, and garlic 1,027 kg per 10a. However, in terms of gross profit, the potato was more than 3.8 times more profitable than corn, sesame was 1.8 times more profitable than perilla, and garlic was more than 2.8 times more profitable than onions. As a result, in terms of net income, the potato-sesame-garlic cropping system produced the highest income per unit area. Sesame seedlings were planted after the potato harvest, thereby solving the problem of competition between the first and last crops. Overall, this study confirmed that the potato-sesame-garlic cropping system, a two-year rotation of three crops, contributed to the improvement of upland crop productivity and farmers' income and was an overall effective cropping system.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.67 no.4
• /
• pp.234-241
• /
• 2022

Wheat (Triticum aestivum L.) is a major staple foods and is in increasing demand in the world. The elevated temperature caused by changes in climate and environmental conditions is a major factor affecting wheat development and grain quality. The optimal temperature range for winter wheat is between 15 and 25℃, and it is necessary to study the physiological characteristic of wheat according to elevated temperatures. This study presents the effect of elevated temperature on the yield and quality of two Korean bread wheat (Baekkang and Jokyoung) in temperature gradient tunnels (TGT). Two bread wheat cultivars were grown in TGT at four different temperature conditions: T0 (control, near ambient temperature), T1 (T0+1℃), T2 (T0+2℃), (T0+2℃), T3 (T0+3℃). The period from sowing to heading stage accelerated and the number of grains per spike and grain yield reduced under T3 condition compared with those under T0 condition. Grain filling rate and grain maturity also accelerated with elevated temperature (T3). The increase in temperature led to the increase in protein contents, whereas decreased the total starch contents. These results are consistent with the decreased expression of starch synthesis genes and increased gliadin synthesis or gluten metabolism genes during the late grain filling stage. Taken together, our results suggest that the increase in temperature (T3) led to the decrease in grain yield by regulating the number of grains/spike, whereas increased the protein content by regulating the expression of starch and gliadin-related genes or gluten metabolism process genes expression. In addition, our results provide a useful physiological information on the response of wheat to heat stress.