• Title/Summary/Keyword: optical sensor

검색결과 2,129건 처리시간 0.028초

Cell-SELEX Based Identification of an RNA Aptamer for Escherichia coli and Its Use in Various Detection Formats

  • Dua, Pooja;Ren, Shuo;Lee, Sang Wook;Kim, Joon-Ki;Shin, Hye-su;Jeong, OK-Chan;Kim, Soyoun;Lee, Dong-Ki
    • Molecules and Cells
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    • 제39권11호
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    • pp.807-813
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    • 2016
  • Escherichia coli are important indicator organisms, used routinely for the monitoring of water and food safety. For quick, sensitive and real-time detection of E. coli we developed a 2'F modified RNA aptamer Ec3, by Cell-SELEX. The 31 nucleotide truncated Ec3 demonstrated improved binding and low nano-molar affinity to E. coli. The aptamer developed by us out-performs the commercial antibody and aptamer used for E. coli detection. Ec3(31) aptamer based E. coli detection was done using three different detection formats and the assay sensitivities were determined. Conventional Ec3(31)-biotin-streptavidin magnetic separation could detect E. coli with a limit of detection of $1.3{\times}10^6CFU/ml$. Although, optical analytic technique, biolayer interferometry, did not improve the sensitivity of detection for whole cells, a very significant improvement in the detection was seen with the E. coli cell lysate ($5{\times}10^4CFU/ml$). Finally we developed Electrochemical Impedance Spectroscopy (EIS) gap capacitance biosensor that has detection limits of $2{\times}10^4CFU/mL$ of E. coli cells, without any labeling and signal amplification techniques. We believe that our developed method can step towards more complex and real sample application.

Mechanical Properties of High Stressed Silicon Nitride Beam Measured by Quasi-static and Dynamic Techniques

  • Shin, Dong Hoon;Kim, Hakseong;McAllister, Kirstie;Lee, Sangik;Kang, Il-Suk;Park, Bae Ho;Campbell, Eleanor E.B.;Lee, Sang Wook
    • 한국진공학회:학술대회논문집
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    • 한국진공학회 2016년도 제50회 동계 정기학술대회 초록집
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    • pp.361.1-361.1
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    • 2016
  • Due to their high sensitivity, fast response, small energy consumption and ease of integration, nanoelectromechanical systems (NEMS) have attracted much interest in various applications such as high speed memory devices, energy harvesting devices, frequency tunable RF receivers, and ultra sensitive mass sensors. Since the device performance of NEMS is closely related with the mechanical and flexural properties of the material in NEMS, analysis of the mechanical and flexural properties such as intrinsic tensile stress and Young's modulus is a crucial factor for designing the NEMS structures. In the present work, the intrinsic mechanical properties of highly stressed silicon nitride (SiN) beams are investigated as a function of the beam length using two different techniques: (i) dynamic flexural measurement using optical interferometry and (ii) quasi-static flexural measurement using atomic force microscopy. The reliability of the results is analysed by comparing the results from the two different measurement techniques. In addition, the mass density, Young's modulus and internal stress of the SiN beams are estimated by combining the techniques, and the prospect of SiN based NEMS for application in high sensitive mass sensors is discussed.

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상관관계를 활용한 집적 영상의 깊이 추출 방법 (Depth Extraction of Integral Imaging Using Correlation)

  • 김영준;조기옥;김철수;조명진
    • 한국정보통신학회논문지
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    • 제20권7호
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    • pp.1369-1375
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    • 2016
  • 본 논문에서는 요소 영상간의 상관관계를 위상필터를 이용하여 구하고 집적 영상 기술에서 이를 활용하여 깊이정보를 추출하는 시스템을 제안한다. 집적 영상 기술은 수동 광원을 사용하여 렌즈 배열을 통해 3차원 물체의 광선정보를 2차원 영상 획득 장치로 기록하고 이를 동일한 렌즈 배열을 통해 3차원 영상을 디스플레이 하는 3차원 영상 시스템 기술이다. 렌즈 배열을 통해 획득된 2차원 영상들은 서로 다른 원근감을 가지는 요소 영상이다. 이 영상들과 물체와의 상관관계를 구하여 깊이정보를 추출할 수 있다. 그리고 요소 영상으로 컴퓨터 재생을 하여 다시 필터를 이용한 상관관계를 구하게 되면 더욱 정확한 깊이정보를 얻을 수 있다. 본 논문에서는 제안된 기술을 검증하기 위해 광학적 실험을 수행하고 상관관계의 지표로써 Peak Side lobe Ratio (PSR)을 구하였다.

침입 감지기능을 가진 다중모드 GRIN(graded-index) 광섬유 내에서의 광파의 전파 (Light Propagation in Multimode GRIN(graded-index) Fibers with Intrusion Sensing Capability)

  • 손영호
    • 센서학회지
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    • 제11권5호
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    • pp.273-278
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    • 2002
  • 광섬유의 침입 감지기능을 개발하기 위하여 다중모드 GRIN(graded-Index) 광섬유의 휨에 의한 광섬유내의 광전파 특성변화를 고찰하였다. 다중모드 CRIN 광섬유의 data파는 기본(fundamental) 모드로, 침입 감시용 감시파는 높은 차수의 모드로 동시에 전파 되게 하고, 광섬유의 휨(bending)에 의한 데이타 유출시도는 높은 차수 모드의 감시파의 감쇠를 가져오고, 결국 수신측의 경보를 작동하도록 한다. 또한 일정한 곡률반경으로 감겨진 광섬유에 기본 모드만 선별적으로 주입된 경우에 있어서, 다중모드 GRIN 광섬유 내에서의 광파의 전파과정을 이론적으로 해석하고, 광섬유가 휘게 피는 경우 여러 모드 사이에 결합(coupling)이 생겨나게 되는데, 광섬유가 감겨진 곡률반경을 변경해가면서 결합되는 기본모드의 power 변화를 수치해석을 이용한 프로그램 시뮬레이션을 통하여 조사하였다. 이 연구결과는 광섬유의 휨에 의하여 방출되는 데이타 도청 및 침입자 감지에 다중모드 GRIN 광섬유 활용 가능성과 광섬유의 곡률반경이 1cm 정도가지는 Asawa-Taylor의 모델[4]이 정당함을 보였다.

다목적실용위성 5호의 SAR 영상 검·보정을 위한 이동형 능동 트랜스폰더 개발 (Development of Mobile Active Transponder for KOMPSAT-5 SAR Image Calibration and Validation)

  • 박덕종;염경환
    • 한국전자파학회논문지
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    • 제24권12호
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    • pp.1128-1139
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    • 2013
  • 다목적실용위성 5호는 영상 레이더(SAR: Synthetic Aperture Radar)을 탑재하여 야간이나 구름이 있는 지역에 대해서도 지속적인 영상촬영이 가능하다. 발사 후 초기 운영 시험에서는 SAR 탑재체에 의해 관측된 영상에 대한 기하 및 복사 보정을 수행한다. 이러한 보정은 지표상의 위치 및 RCS(Radar Cross Section)을 알고 있는 보정장치에서 반사된 SAR 신호에 의해 나타난 영상 픽셀에서의 위치 및 값을 이용하여 처리된다. 이러한 보정을 위해 기존 반사기와 달리 능동 트랜스폰더는 RCS 값을 변경할 수 있으며, 내부 시간 지연 기능에 의해 가상의 위치로 이동하는 효과를 영상에서 나타낼 수 있다. 본 논문에서는 능동 트랜스폰더의 개발에 필요한 요구사항을 도출하여 이로부터 설계, 조립 및 시험결과를 정리하였다.

사파이어 화학기계적 연마에서 결정 방향이 재료제거 특성에 미치는 영향 (Effect of Crystal Orientation on Material Removal Characteristics in Sapphire Chemical Mechanical Polishing)

  • 이상진;이상직;김형재;박철진;손근용
    • Tribology and Lubricants
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    • 제33권3호
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    • pp.106-111
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    • 2017
  • Sapphire is an anisotropic material with excellent physical and chemical properties and is used as a substrate material in various fields such as LED (light emitting diode), power semiconductor, superconductor, sensor, and optical devices. Sapphire is processed into the final substrate through multi-wire saw, double-side lapping, heat treatment, diamond mechanical polishing, and chemical mechanical polishing. Among these, chemical mechanical polishing is the key process that determines the final surface quality of the substrate. Recent studies have reported that the material removal characteristics during chemical mechanical polishing changes according to the crystal orientations, however, detailed analysis of this phenomenon has not reported. In this work, we carried out chemical mechanical polishing of C(0001), R($1{\bar{1}}02$), and A($11{\bar{2}}0$) substrates with different sapphire crystal planes, and analyzed the effect of crystal orientation on the material removal characteristics and their correlations. We measured the material removal rate and frictional force to determine the material removal phenomenon, and performed nano-indentation to evaluate the material characteristics before and after the reaction. Our findings show that the material removal rate and frictional force depend on the crystal orientation, and the chemical reaction between the sapphire substrate and the slurry accelerates the material removal rate during chemical mechanical polishing.

Au와 탄소나노튜브 복합체 전극의 연성 향상 (Enhanced Stretchability of Gold and Carbon Nanotube Composite Electrodes)

  • 우정민;전주희;강지연;이태일;명재민
    • 한국재료학회지
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    • 제21권3호
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    • pp.133-137
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    • 2011
  • Gold have been used as an electrode materials having a good mechanical flexibility as well as electrical conductivity, however the stretchability of the gold on a flexible substrate is poor because of its small elastic modulus. To overcome this mechanical inferiority, the reinforcing gold is necessary for the stretchable electronics. Among the reinforcing materials having a large elastic modulus, carbon nanotube (CNT) is the best candidate due to its good electrical conductivity and nanoscale diameter. Therefore, similarly to ferroconcrete technology, here we demonstrated gold electrodes mechanically reinforced by inserting fabrics of CNTs into their bodies. Flexibility and stretchability of the electrodes were determined for various densities of CNT fabrics. The roles of CNTs in resisting electrical disconnection of gold electrodes from the mechanical stress were confirmed using field emission scanning electron microscope and optical microscope. The best mechanical stability was achieved at a density of CNT fabrics manufactured by 1.5 ml spraying. The concept of the mechanical reinforced metal electrode by CNT is the first trial for the high stretchable conductive materials, and can be applied as electrodes materials in various flexible and stretchable electronic devices such as transistor, diode, sensor and solar cell and so on.

투명한 p형 반도체 CuAlO2 박막의 일산화질소 가스 감지 특성 (Nitrogen Monoxide Gas Sensing Characteristics of Transparent p-type Semiconductor CuAlO2 Thin Films)

  • 박수정;김효진;김도진
    • 한국재료학회지
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    • 제23권9호
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    • pp.477-482
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    • 2013
  • We investigated the detection properties of nitrogen monoxide (NO) gas using transparent p-type $CuAlO_2$ thin film gas sensors. The $CuAlO_2$ film was fabricated on an indium tin oxide (ITO)/glass substrate by pulsed laser deposition (PLD), and then the transparent p-type $CuAlO_2$ active layer was formed by annealing. Structural and optical characterizations revealed that the transparent p-type $CuAlO_2$ layer with a thickness of around 200 nm had a non-crystalline structure, showing a quite flat surface and a high transparency above 65 % in the range of visible light. From the NO gas sensing measurements, it was found that the transparent p-type $CuAlO_2$ thin film gas sensors exhibited the maximum sensitivity to NO gas in dry air at an operating temperature of $180^{\circ}C$. We also found that these $CuAlO_2$ thin film gas sensors showed reversible and reliable electrical resistance-response to NO gas in the operating temperature range. These results indicate that the transparent p-type semiconductor $CuAlO_2$ thin films are very promising for application as sensing materials for gas sensors, in particular, various types of transparent p-n junction gas sensors. Also, these transparent p-type semiconductor $CuAlO_2$ thin films could be combined with an n-type oxide semiconductor to fabricate p-n heterojunction oxide semiconductor gas sensors.

인치웜 리니어 모터 시스템 설계 및 제작에 관한 연구 (A Study on Design and Manufacture of an Inchworm Linear Motor System)

  • 예상돈;정재훈;민병현
    • 한국정밀공학회지
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    • 제21권12호
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    • pp.174-181
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    • 2004
  • Ultra precision positioning mechanism has widely been used on semiconductor manufacturing equipments, optical spectrum analyzer and cell manipulations. Ultra precision positioning mechanism is consisted of several actuators, sensors, guides and control systems. Its efficiency depends on each performance of components. The object of this study is to design, analysis and manufacture all of the inchworm linear motor system, which is one of the equipments embodied in ultra precision positioning mechanism. Inchworm linear motor system is consisted of a controller system and an inchworm linear motor, and its driving form is similar to a motion of spanworm. A design and manufacture of inchworm linear motor, which is consisted of three PZT actuators, a rod, two columns and a guide plate, are performed. Minimizing the von-Mises stress of the hinge using Taguchi method and simulation by FEM software optimizes the structural design in a column of flexure hinge. The designed columns and guide plates are manufactured by a W-EDM and NC-milling. A controller system, which is an apparatus to drive inchworm linear motor, can easily adjust driving conditions by varying resonance frequency and input-output voltage of actuators and amplifiers. The performance of manufactured inchworm linear motor system is verified and valuated. In the future, inchworm linear motor system will be used to make a more precision positioning by reinforcing a sensor and feedback system.

UV KrF 레이저 공정조건에 따른 FBG 센서의 방사선 영향 (Radiation Effects on Fiber Bragg Grating Sensors Written in UV KrF Laser Process Condition)

  • 김종열;이남호;정현규
    • 한국정보통신학회논문지
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    • 제20권1호
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    • pp.161-166
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    • 2016
  • 본 논문에서는 광섬유 브래그 격자의 공정조건 변화에 따른 $Co^{60}$ 감마방사선 영향을 연구하였다. 광섬유 브래그 격자는 붕소가 첨가된 광민감 광섬유를 이용하여 UV KrF 레이저 세기를 달리하여 제작하였으며, 제작된 광섬유 브래그 격자 센서에 총 선량 33.8 kGy 감마선을 조사하였다. 실험결과를 통하여, 격자 공정을 위한 UV 레이저 세기가 광섬유 브래그 격자의 방사선 민감도에 큰 영향을 줄 수 있다는 것을 확인하였으며, 레이저 공정조건 변화에 따른 방사선에 의한 광섬유 브래그 파장의 변화는 30 % 이상의 차이를 보였다.