• Journal of Broadcast Engineering
• /
• v.13 no.5
• /
• pp.612-621
• /
• 2008

Due to the various constraints such as feasible size of data payload and low transmission power, no technical specifications on the voice communication are included in the Zigbee standard. In this paper, a voice coding technique for application to the IEEE 802.15.4 standard, which is the basis of Zigbee communication, is presented. Here, both high compression and good waveform recovery are essential. To meet those requirements, a multi-stage discrete wavelet transform (DWT) block and a binary coding block consisting of two different pulse-code modulations are exploited. Theoretical analysis and simulation results in an indoor wireless channel show that the voice coder with 2-stage DWT is most appropriate from the viewpoint of compression and waveform recovery. When the line-of-sight component is dominant, the voice coding scheme has good recovery capability even in the moderate signal-to-noise power ratios. Hence, it is considered that the presented scheme will be a technical reference for the future recommendation of voice communication exploiting Zigbee.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.13 no.1
• /
• pp.1-6
• /
• 1993

This experiment was conducted to select acid tolerant cells from red clover suspension culture and obtained following results. The most effective condition of plant growth regulators for suspension culture of red clover cells was 2 mg/l 2,4-D as auxin source plus 0.5 mg/l BAP as cytokinin source. Among serveral basal media, PC medium brought the best result. The most suitable EMS concentration for the mutated cells tolerant to acid was 1 % with fours treatment and average 27 colonies/plate were selected. The frequency of the occurence of mutants tolerant to acid in 1 % EMS with four hours treatment was 8.9 $\times$ 10$^{-5}$ which was 100 times larger than that of control. The total colonies selected initially were 176 but the survived colonies after two subcultures with 4 weeks interval in a selection medium were 44.

• The Journal of the Korean dental association
• /
• v.46 no.10
• /
• pp.623-632
• /
• 2008

Purpose : The purpose of this study is to identify the effect of zoledronate(Zometa(R)), which is most common nitrogen containing bisphosphonate, on survival, proliferation, and differentiation of osteoblast. Material & Methods: Twenty four cell culture plates containing essential medium were seeded with UMR-106 cell lines, at density of 5 x $10^4 cells per plates. Each plates were incubated with 5% $CO^2 incubator at $37^{\circ}C$. Starting from 2 days after incubation, cell culture medias were replaced, and added with osteogenesis induction media and 0, 0.01, 0.1, 0.5, 1, $3\muM$ of zoledronate(Zometa(R)), every 2 days, for 12 days. Control group was plates not added with zoledronate($0\muM$), and experiment group were plates added with different concentration of zoledronates(0, 0.01, 0.1, 0.5, 1, $3\muM$). Mature osteoblasts were identified with Alizarine Red staining, and protein samples were collected. Optical density was determined at wavelength of 405nm with ELISA reader. For viability analysis, cells were harvested and incubated with propidium iodide, and analysed with flow cytometry. Western blot technique was used to analyse Runx2 protein of osteoblast. Results : Secretion of bone matrix decreased as zoledronate concentration increased, and zoledronate did not effect survival rate of UMR-106 cells when measured with flow cytometer. Expression of Runx2 protein was inhibited as zoledronate concentration increased. Conclusion : From the results, we were able to identify that increase of zoledronate concentration inhibited differentiation of UMR-106 cell to osteoblast, without effecting quantity or survival rate.

• Journal of Microbiology and Biotechnology
• /
• v.6 no.6
• /
• pp.474-481
• /
• 1996

Since the commercially available rabbit anti-cyclin D3, generated from c-terminal 16 amino acid residues which are common to human and murine cyclin D3, is highly cross-reactive with many other cellular proteins of mouse, a new rabbit polyclonal anti-cyclin D3 has been raised by using murine cyclin D3 protein expressed at a high level in Escherichia coli as the immunogen. To express murine cyclin D3 protein in E. coli, the cyclin D3 cDNA fragment encoding c-terminal 236 amino acid residues obtained by polymerase chain reaction (PCR) was inserted into the NcoI/BamHI site of protein expression vector, pET 3d. Molecular mass of the cyclin D3 overexpressed in the presence of IPTG (Isopropyl $\beta$-D-thiogalactopyranoside) was approximately 26 kDa as calculated from the reading frame on the DNA sequence, and the protein was insoluble and mainly localized in the inclusion bodies that could be easily purified from the other cellular soluble proteins. When renaturation was performed following denaturation of the insoluble cyclin D3 protein in the inclusion bodies using guanidine hydrochloride, 4.4 mg of soluble form of cyclin D3 protein was produced from the transformant cultured in 100ml of LB media under the optimum conditions. Four-hundred micrograms of the soluble form of cyclin D3 protein was used for each immunization of a rabbit. When the antiserum obtained 2 weeks after tertiary immunization was applied to Western blot analysis, it was able to detect 33 kDa cyclin D3 protein in both murine lymphoma cell line BW5147.G.1.4 and human Jurkat T cells at 3,000-fold dilution with higher specificity to murine cyclin D3, demonstrating that the new rabbit polyclonal anti-murine cyclin D3 generated against c-terminal 236 amino acid residues more specifically recognizes murine cyclin D3 protein than does the commercially available rabbit polyclonal antibody raised against c-terminal 16 amino acids residues.

• The Research Journal of the Costume Culture
• /
• v.11 no.6
• /
• pp.808-825
• /
• 2003

The purpose of this study is to research the reasons of this outcome by comparing the consumers purchasing characteristics of the Internet shopping mall and cable TV home shopping. A questionnaire was designed for on-line survey panels residing in Korea and the period of the survey was from July 30 to August 6, 2002. 1362 questionnaires were collected and 665 were chosen. The Frequency, %, t-test, and x2-test was performed using Korean version of SPSS 10.0. The summarized results of this study are as follows: The reasons of purchasing Apparel products through Internet and Cable TV home shopping are low price, time Saving, and convenience. The Item that is purchased most often at the Internet shopping mall is casual T-shirt. In case of the cable TV home shopping, it is inner-wears. Most of the users were satisfied with the home shopping. There are significant difference in ‘inexpensiveness of price’, ‘variety of product’, ‘sufficiency of product information’, ‘easiness of searching product’, ‘security of personal information’, ‘convenience of order’ in the side of consumers satisfaction. The reason of not using the media shopping are as follows: ‘Distrust of the product’ appeared one of the main reason of not using internet shopping mall and cable TV home shopping, accounting for 34.3%(Internet)/25.3%(Cable TV) of all respondents, followed by ‘absence of products(18.7%)’, In case of the cable TV home shopping, ‘lack of necessity of home shopping(24.7%)’ was the main reason.

• Reproductive and Developmental Biology
• /
• v.28 no.1
• /
• pp.7-12
• /
• 2004

This study was performed to establish the effective culture condition for the establishment of clonal lines from porcine fetal fibroblast cells. Fibroblasts derived from a pig fetus (Day 50) were cultured and passaged two times before use. A single cell was seeded in 96-well plates, cultured in medium supplemented with different concentrations of FBS, catalase or $\beta$-mercaptoethanol ($\beta$ME), and classified by cell size and morphology. Cells were passaged two times into 4-well dish before freezing. The establishment efficiencies were not different among different concentrations of FBS (0.3 to 5.1%). However, population doubling time (PDT) was significantly decreased by increasing the FBS concentration (P＜0.05). The establishment efficiency of $\beta$ME-added group (10.4%) was significantly higher than those of catalase-added and control groups (3.5%, and 3.5%, respectively, p＜0.05), and PDT was significantly decreased (23.6 vs 28.1, and 25.5 h, respectively, p＜0.05). However, catalase did not show a positive effect on the establishment efficiency. Cell size and morphology did not affect the establishment efficiency and PDT of clonal lines. The result of present study shows that the establishment efficiency of clonal cell lines can be enhanced by the culture in media supplemented with 30% FBS and $\beta$ME.

• Journal of Broadcast Engineering
• /
• v.24 no.6
• /
• pp.992-1001
• /
• 2019

In many applications, images acquired from multiple cameras are stitched to form an image with a wide viewing angle. We propose a method of estimating a seam line using motion information to stitch multiple images without distortion of the moving object. Existing seam estimation techniques usually utilize an energy function based on image gradient information and parallax. In this paper, we propose a seam estimation technique that prevents distortion of moving object by adding temporal motion information, which is calculated from the gradient information of each frame. We also propose a measure to quantify the distortion level of stitched images and to verify the performance differences between the existing and proposed methods.

• Journal of KIISE:Software and Applications
• /
• v.31 no.2
• /
• pp.203-212
• /
• 2004

The watermarking is a method to achieve the copyright protection of multimedia contents. Among several media, the left documents show very peculiar properties: block/line/word patterning, clear separation between foreground and background areas. So algorithms specific to the text documents are required that meet those properties. This paper proposes a novel watermarking algorithm for the grayscale text document images. The algorithm inserts the watermark signals through the edge direction histograms. A concept of sub-image consistency is developed that the sub-images have similar shapes in terms of edge direction histograms. Using Korean, Chinese, and English document images, the concept is evaluated and proven to be valid over a wide range of document images. To insert watermark signals, the edge direction histogram is modified slightly. The experiments were performed on various document images and the algorithm was evaluated in terms of imperceptibility and robustness.

• Journal of Advanced Navigation Technology
• /
• v.14 no.6
• /
• pp.824-830
• /
• 2010

PLC_Zigbee system, implemented in a previous study, uses ZigBee Node based on PLC (Power Line Communication). The weak point of the system is that the long distance powerline communication and PLC is impossible to use in the areas where the powerline is not installed. In this paper, we added CDMA (Code Division Multiple Access) module using a data transfer method of SMS (Short Message Service) and XBee of WSN (Wireless Sensor Network) module to the previous system, and got around the restriction of place and environment of PLC system through the interfacing of each communication media. We, thus, implemented a wide range of real monitoring system.

• Journal of the Korea Institute of Information and Communication Engineering
• /
• v.23 no.12
• /
• pp.1551-1557
• /
• 2019

In modern society, a variety of video media have been widely spread in line with the fourth industrial revolution and the development of IoT technology; in accordance with this trend, numerous researches have been performed to remove noise generated in image and data communications. However, the conventional Additive White Gaussian Noise (AWGN) cancellation techniques are likely to induce a blurring phenomenon in the noise removal process, thus impairing the information of the image. In this study, we propose an algorithm for minimizing the loss of image information in the removal process of AWGN. The proposed algorithm can apply weights according to the characteristics of noise by predicting AWGN in the image, where the output is calculated based on adding and subtracting the outputs of the high pass filter and the low pass filter. Compared to the existing method, the noise reduction using the proposed algorithm exhibited less blurring issues and better noise reduction properties in the AWGN removal process.