Journal of Microbiology and Biotechnology
- Volume 6 Issue 6
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- Pages.474-481
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- 1996
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- 1017-7825(pISSN)
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- 1738-8872(eISSN)
Rabbit Antibody Raised against Murine Cyclin D3 Protein Overexpressed in Bacterial System
- Jun, Do-Youn (Department of Microbiology, College of Natural Sciences Kyungpook National University) ;
- Kim, Mi-Kyung (Department of Microbiology, College of Natural Sciences Kyungpook National University) ;
- Kim, Young-Ho (Department of Microbiology, College of Natural Sciences Kyungpook National University)
- Published : 1996.12.01
Abstract
Since the commercially available rabbit anti-cyclin D3, generated from c-terminal 16 amino acid residues which are common to human and murine cyclin D3, is highly cross-reactive with many other cellular proteins of mouse, a new rabbit polyclonal anti-cyclin D3 has been raised by using murine cyclin D3 protein expressed at a high level in Escherichia coli as the immunogen. To express murine cyclin D3 protein in E. coli, the cyclin D3 cDNA fragment encoding c-terminal 236 amino acid residues obtained by polymerase chain reaction (PCR) was inserted into the NcoI/BamHI site of protein expression vector, pET 3d. Molecular mass of the cyclin D3 overexpressed in the presence of IPTG (Isopropyl
Keywords
- murine cyclin 3D protein;
- overexpression in E. coli;
- inclusion body;
- renaturation;
- polyclonal anti-murine cyclin D3 antibody