Reproductive and Developmental Biology

The Korean Society of Animal Reproduction (한국동물번식학회)
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Culture of Clonal Lines in Porcine Fetal Fibroblast Cells

돼지 태아섬유아세포 Clonal Lines의 배양

  • Kwon, D. J. (College of Animal Resource Science, Kangwon National University) ;
  • Park, C. K. (College of Animal Resource Science, Kangwon National University) ;
  • B. K. Yang (College of Animal Resource Science, Kangwon National University) ;
  • Kim, C. I. (College of Animal Resource Science, Kangwon National University) ;
  • H. T. Cheong (College of Animal Resource Science, Kangwon National University)
  • Published : 2004.03.01
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Abstract

This study was performed to establish the effective culture condition for the establishment of clonal lines from porcine fetal fibroblast cells. Fibroblasts derived from a pig fetus (Day 50) were cultured and passaged two times before use. A single cell was seeded in 96-well plates, cultured in medium supplemented with different concentrations of FBS, catalase or $\beta$-mercaptoethanol ($\beta$ME), and classified by cell size and morphology. Cells were passaged two times into 4-well dish before freezing. The establishment efficiencies were not different among different concentrations of FBS (0.3 to 5.1%). However, population doubling time (PDT) was significantly decreased by increasing the FBS concentration (P＜0.05). The establishment efficiency of $\beta$ME-added group (10.4%) was significantly higher than those of catalase-added and control groups (3.5%, and 3.5%, respectively, p＜0.05), and PDT was significantly decreased (23.6 vs 28.1, and 25.5 h, respectively, p＜0.05). However, catalase did not show a positive effect on the establishment efficiency. Cell size and morphology did not affect the establishment efficiency and PDT of clonal lines. The result of present study shows that the establishment efficiency of clonal cell lines can be enhanced by the culture in media supplemented with 30% FBS and $\beta$ME.

Keywords

References

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