• Applied Microscopy
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• v.32 no.1
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• pp.39-44
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• 2002

The ultrastructure of the fertilized egg envelope from dark sleeper, Odontobutis obscurus interrupta belong to Eleotrididae was studied using scanning and transmission electron microscopes. The fertilized egg of dark sleeper was of transparent, ellipsoidal, adhesive and demersal type, and there were numerous oil droplets in the yolk sac and adhesive filaments in the area of the animal pole. The outer surface of egg envelope was smooth with pore canal. The fertilized egg envelope consisted of two layers, an outer nonadhesive layer and an inner layer, consisting of seven horizontal low electron-dense lamellae alternating with the middle electron dense interlamellae. These ultrastructural characters of fertilized egg envelope from dark sleeper can be utilized in taxonomy of teleost.

• Korean Journal of Ichthyology
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• v.26 no.4
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• pp.267-273
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• 2014

This research has carried out to make a data base of taxonomic research. That Data base explains about spawning behavior, egg development, and morphologic development of variable sabretooth blenny. Fertilized egg was demersal egg which is white and opaque. The number of oil glouble were 10~11, and the size of egg was 0.90~1.43 mm (average rate $1.11{\pm}0.23mm$, n=10). Breeding water temperature was $25.5{\sim}28.5^{\circ}C$ (average rate $27.0^{\circ}C$), and salinity was 32.5~33.5‰ (average rate 33.0‰). After 24 hours from 2 cells, the process of egg development was reached to Blastula stage. Moreover, after 330 hours from 2 cells, nostrils and eyes were formed. Egg membrane was pierced by the head, and the hatch began. After the hatch, postlarvae had 2.59~3.02 mm (average rate $2.81{\pm}0.25mm$, n=5) of whole length, and the mouth and anus were opened. Yolk sac and oil glouble were absorbed. After three days from hatch, prelarvae were 3.02~3.07mm(average rate $3.04{\pm}0.04mm$) of whole length, and caudal fin was grown with round shape. After 13 days from hatch, prelarvae had 3.04~3.20 mm (average rate $3.12{\pm}0.11mm$) of whole length, and they could eat food with upper jaw and bottom jaw.

• Korean Journal of Ichthyology
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• v.12 no.2
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• pp.137-145
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• 2000

The embryonic and larval development of Chelon lauvergnii (Eydoux & Souleyet) was surveyed by incubating artificially inseminated eggs with parent fishes obtained at Kang-wha island in the mid-western coastal area of Korea on June, 1997. The fertilized eggs were transparent, spherical in shape, measuring 0.95~1.08 mm in diameter, having a large oil globule, and their perivitelline space narrow, and began to hatch at 40 hrs. in water temperature $22{\pm}1^{\circ}C$. The newly hatched larvae were 2.35~2.68 mm in total length with 23 myomeres, anus opened, mouth closed, preanal length 58.7~61.6% of total length, oil globule located in posterior end of yolk sac. Melanophores, branch in shape, were distributed mainly along the ventro-lateral region of trunk part and a few on the anterior end of caudal part and surface of oil globule. The larvae measuring 3.08~3.36 mm in total length absorbed yolk material completely in 3 days after hatching, in which air bladder began to appear and mouth opened. In 8 days after hatching, the larva was measured 5.09 mm in total length, its posterior end of notochord began to flex upward and the caudal fin rays differentiated as 7, finfold of the second dorsal and anal fins appeared. In this time, melanophores, branch in shape, were concentrated in the anterior half region of the caudal part and a few also distributed on the top of head, snout region, ventral margin of lower jaw and isthmus region. In 12 days after hatching, the larva measuring 8.48 mm in total length completed all the fins (D. IV-9; P1. 16; P2. I, 5; A. II, 9) and reached to the juvenile stage. Melanophores, in this time, were distributed on the mid-lateral region of the caudal part in enlargment than before and a few also found in the dorso-lateral region of the trunk part, and in the cheek region.

• Korean Journal of Ichthyology
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• v.1 no.1_2
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• pp.1-8
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• 1989

In August, 1988, matured adults of devil stinger were collected from Jinhae Bay and Jaran Bay, Kyongsangnam-do, Korea and kept in the aquarium (1 ton) for spawning. The egg development was observed with the eggs laid in the aquarium of adult fish. The diameters of eggs ranged from 1.2 to 1.3 mm (n=6), and no oil globules were found in the eggs. The hatching took place from 26 to 29 hours after eight cells stage at the water temperature of $24.9-26.5^{\circ}C$. The newly hatched larvae were 2.47~3.10 mm in total length with xanthophore on the body and yolk sac. Myomere number was 13+16-17=29-30. Two days after hatching, larvae were 3.61-4.16 mm in total length, and 3 to 4 large spots of melanophore appeared on the large pectoral fin. Three days after hatching, larvae were 3.97-4.29 mm in total length, and the larvae absorved the yolk material completely to become post larvae with 4-5 rays of the pectoral fin. Six days after hatching, larvae attained 4.07-5.46 mm in total length, and 11 rays and 8-9 spots of melanophore were developed on the pectoral fin.

• Korean Journal of Ichthyology
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• v.30 no.1
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• pp.18-26
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• 2018

We investigated to egg development and early life history of Tribolodon brandtii in the Korean peninsula. The fertilized eggs of the T. brandtii were slightly adhesive and separated with a yellow yolk and no oil globule, and they was an average of $2.4{\pm}0.2mm$ in diameter. The hatching of the embryo took place in about 60 hrs after fertilization under water temperature of $20{\pm}1^{\circ}C$ and the newly hatched larvae were average $6.4{\pm}0.4mm$ in total length. At 10 days after hatching, they were average $11.7{\pm}0.2mm$ in total length and their yolk sac were completely absorbed. At 46 days after hatching, they became to juvenile stage and reached $19.6{\pm}2.7mm$ in total length and all their fin rays were formed. At 125 days after hatching, the juvenile were $54.9{\pm}15.8mm$ in total length and their body shape were similar to the adult fish. At 236 days after hatching, they fish were $54.9{\pm}15.8mm$ in total length and their body color were similar to the adult fish.

• Journal of Aquaculture
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• v.11 no.1
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• pp.49-58
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• 1998

The spawning behavior and early life history of the Korean aucha perch, Coreoperca herzi were studied. The eggs were spawned in a one-layer mass, hanging from the underside of a largy stone and guarded by one male. According to the considerable size difference os eggs in the individual ovaries, it was concluded that they spawns several times within a spawning season of May~June. The ripe eggs were demersal and adhesive, and their diameter were 2.60~3.04mm with several oil globule of 0.10~0.64mm. Hatching in the tank with $19.0{\pm}1.0^{\circ}C.$ in men water temperature started from the 380 hours after fertilization. Newly-hatched larvae were measured 6.45~7.34mm in total length (TL, mean : 6.85mm), and mouth and anus were open. Melanophores were present on the head, around the yolk, and on the dorsal part of the abdominal region. 3 days old larvae were measured 7.00~7.40mm in TL (mean : 7.25mm), and they fed Daphnia sp. and Brachionus plicatilis actively. 6 days old larvae transformed to postlarval stage and they were 7.94~10.10mm in TL (mean : 9.00mm). As yolk sac were nearly absorbed, the caudal notochord flex at 45. Aggregate numbers of all fin rays were completed at over 16.90~19.80mm in TL (30 days after hatching), at which time the larvae reached the juvenile stage. In fifty days old larvae(24.60~27.10mm in TL) were similar in body form, lateral line and color to adult.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.15 no.4
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• pp.323-328
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• 1982

Kareius bicoloratus (Basilewsky) is one of the commonly found right-eye flounders and widely distributed in the coastal waters of Korea and Japan. On December 11,1980, the ailthors carried out an experiment to obtain a large number of fertilized eggs from wild adult fish caught by a trawler. The fish were obtained from Maisaka fish market, Shizuoka Prefecture, Japan. The egg is pelagic, spherical in shape and measuring 1.014-1.04 mm in diameter. The yolk as well as the egg capsule is colorless and transparent, and contain no oil globules. The hatching took place in 73 hr 45 min after fertilization at the water temperature $8.0-9.5^{\circ}C$. Newly hatched larvae are 3.09-3.146 mm in total length, with the anus situated in the middle of the body. The marginal fin does not have Pigment cells and myotome number is 17+20=37. Within one day after hatching, the larvae attained 3.77 mm in total length, and there appeared three or four melanophore on yolk sac. When the larvae attained 3.96 mm in total length, melanophores began to appear on the eye ball. Two days after hatching, the larvae attained 4.05 mm in total length, most of yolk material was absorbed, and the caudal fin began to grow at the terminal part of the notochord. When the larvae attained 4.21 mm in total length, mouth and eyes began to move. After 3 days, the larvae attained 4.342-4.394 mm in total length, alimentary canal differentiated, melanophores appeared on the lower jaw and posterior part of the fin membrane. When the larvae attaind 4.576 mm in total length, marginal line of dorsal fin membrane became concave.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.19 no.4
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• pp.380-386
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• 1986

Snailfish, Liparis tanakai (Gilbert et Burke) is widely distributed in the coastal waters of Korea and Japan. On December 21, 1985, fertilized eggs of the fish were collected from the coastal waters of Saryang-do island, Kyongsangnam-do, Korea, and the eggs were incubated and the larvae reared at a mean water temperature of $9.2^{\circ}C$ in the laboratory. The fertilized eggs of this species are demersal, adhesive and spherical in shape, measuring $1.68{\sim}1.78mm$ (mean 1.72mm, n=30) in diameter, containing about 20 oil globules. Hatching took place 21 days after blastula stage. During the incubation period water temperature fluctuated between $8.1^{\circ}C\;and\;11.8^{\circ}C$. The newly hatched larvae were $5.70{\sim}6.41mm$ (mean 6.10mm, n=30) in total length with $7{\sim}9$ (abdominal) and 36 (caudal) myomeres. Many melanophores were distributed on the large pectoral fin, yolk sac and peritoneum. Twelve days after hatching, the larvae attained $5.85{\sim}6.46mm$ in total length, and at this time the larvae absorbed the yolk almost completely. Melanophores appeared on the snout and the posterior part of head.

• The Korean Journal of Zoology
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• v.31 no.3
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• pp.191-206
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• 1988

Chick embryos which have received a single injection of the organophosphate compound,malathion (0.1 mg, 0.5 mg, 1.0 mg or 2.0 mg in 0.05 ml of corn oil) via the yolk sac at certain times (2 daya, 4 days or 6 days after incubation) have been investigated. After 9 days of incubation, chick embryos were harvested to investigate the effects of malathion on the development of cerebrum morphologically and biochemically. The effects of simultaneous injection of malathion and nicotinamide were also compared. On ultrastructural examination, neurons in cerebral cortex showed to be inhibited in their differentiation by malathion; nuclear irregularity, swelling of endoplasmic reticulum, and cytoplasmic vacuoles were observed. On cytochemical study of acetylcholinesterase(AChE) by electron microscope, the positive reaction products of this enryme were localized at the membrane of nucleus and endoplasmic reticulum of neurons. Inhibition of AChE activty was severe in groups treated with relatively low doses, which was consistent with the results of spectrophotometric analysis. The activity of lactate dehydrogenase(LDH) of cerebrum in groups treated with malathion was higher than that of the control group. The nicotinamide adenine dinucleotide(NAD) content of chick embruo treated with malathioti decreased significantly, and nicotinamide coinjection raised the NAD level as compared with the control group, thus preventing malathion-induced momhological alteration. In conclusion, it is suggested that malathion changes the ultrastructure of differentiating neurons and alters some enzyme activities in chick embryo cerebrum, and the severity of which is consistently dose-or age-dependent.

• Korean Journal of Ichthyology
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• v.25 no.4
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• pp.200-207
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• 2013

We investigated developmental stages of egg and early life history of the Korean indigenous fish, Korean Chub Zacco koreanus from the South Han River in 2011 for phylogenetic study and conservation of this species. Eggs of Zacco koreanus were artificially fertilized by the dry method in the laboratory. The fertilized eggs were demersal, almost spherical in shape, transparent and unpigmented, with a pale yellow yolk, and no oil globule and average $3.09{\pm}0.07$ mm (n=10) in diameter. The hatching of the embryo took place in about 68 hrs after fertilization under water temperature of $20.0{\sim}23.0^{\circ}C$ and the newly hatched larvae were average $10.30{\pm}0.40$ mm (n=10) in total length (TL). Six days after hatching, the larvae grew up to $16.12{\pm}0.42$ mm (n=8) in TL and york sac absorption, mouth and anus opening were shown postflexion larvae stage. 17 days after hatching, most of fin-rays appeared at $18.21{\pm}0.38$ mm (n=6) in TL and brown spot appeared on the abdomen. 27 days after hatching, the larvae were brought up to $20.01{\pm}1.12$ mm(n=5) in TL and all their fin-rays were formed. 120 days after hatching, the larvae (juvenile) were $23.29{\pm}3.12$ mm (n=10) in TL and their body shape and color pattern were similar to the adult fish.