Ultrastructure of the Fertilized Egg Envelope from Dark Sleeper, Eleotrididae, Teleost

경골어류 구굴무치과 얼룩동사리의 수정란 난막 미세구조

  • Kim, Dong-Heui (Dept. of Basic Science and Institute of Basic Medical Science, Wonju College of Medicine, Yonsei Univ.) ;
  • Reu, Dong-Suck (Dept. of Biology, Chongju Univ.) ;
  • Deung, Young-Kun (Dept. of Basic Science and Institute of Basic Medical Science, Wonju College of Medicine, Yonsei Univ.)
  • 김동희 (연세대학교 원주의과대학 기초과학교실.연세대학교 기초의학연구소) ;
  • 류동석 (청주대학교 생물학과) ;
  • 등영건 (연세대학교 원주의과대학 기초과학교실.연세대학교 기초의학연구소)
  • Published : 2002.03.01

Abstract

The ultrastructure of the fertilized egg envelope from dark sleeper, Odontobutis obscurus interrupta belong to Eleotrididae was studied using scanning and transmission electron microscopes. The fertilized egg of dark sleeper was of transparent, ellipsoidal, adhesive and demersal type, and there were numerous oil droplets in the yolk sac and adhesive filaments in the area of the animal pole. The outer surface of egg envelope was smooth with pore canal. The fertilized egg envelope consisted of two layers, an outer nonadhesive layer and an inner layer, consisting of seven horizontal low electron-dense lamellae alternating with the middle electron dense interlamellae. These ultrastructural characters of fertilized egg envelope from dark sleeper can be utilized in taxonomy of teleost.

한국어류의 수정란 난막 미세구조를 분류체계에 따른 체계화 작업의 일환으로 난문의 유무, 난막의 표면 및 단면구조를 밝힘으로써 계통분류학적 기초자료를 제시하고자 경골어류, 구굴무치과에 속하는 얼룩동사리의 수정란을 실험재료로 하여 주사전자현미경과 투과전자현미경을 이용하여 관찰하였다. 수정란은 부착성 및 침성란으로 장타원형이었으며 동물극쪽에 부착사가 부착되어 있었다. 난황낭 내에는 많은 유적들이 있었으며, 난막 표면은 pore cannal들이 분포하고, 난문은 관찰되지 않았다. 난막은 두 층으로 구성되어 있었는데 외층은 전자밀도가 높은 비부착성이었으며, 내층은 전자밀도가 서로 다른 7층으로 구성되어 있었다. 이상과 같이 얼룩동사리 수정란 난막의 미세구조적 특징은 얼룩동사리의 수정란이 가지는 독특한 형태학적 형질로서 종을 분류하는데 사용될 수 있을 것으로 사료된다.

Keywords

References

  1. Deung YK, Reu DS, Kim DH: Comparative ultrastructures of the fertilized egg envelopes in golden severum, convic cichlid and discus, Cichlidae, Teleost, Korean J Electron Microscopy 27 (4): 417-432, 1997. (Korean)
  2. Deung YK, Kim DH, Reu DS: Ultrastructure of gametes in the Three-spine stickleback, Gasterosteus aculeatus aculeatus, Korean J. Electron Microscopy 29 (2): 177-187, 1999. (Korean)
  3. Deung YK, Kim DH, Reu DS: Ultrastructure of the fertilized egg envelope in pale chub Cyprinidae, Teleost, Korean J Electron Microscopy 30 (4): 321-326, 2000. (Korean)
  4. Guraya SS: Monographs in developmental biology, The cell and molecular biology of fish oogenesis, Karger 18: 111-147, 1986
  5. Ivankov VN, Kurdyayeva VP: Systematic differences and the ecological importance of the membranes in fish eggs, J Ichthyol 13: 864-873, 1973
  6. Kim DH, Reu DS, Deung YK: A comparative study on the ultrastructure of the egg envelope in fertilized eggs of fishes, Characidae, three species, Korean J Electron Microscopy 26 (3): 277-291, 1996. (Korean)
  7. Kim DH, Reu DS, Deung YK: Comparative ultrastructure of the fertilized egg envelope in three species, Cyprinidae, Teleost, Korean J Electron Microscopy 28 (2): 237-253, 1998a. (Korean)
  8. Kim DH, Reu DS, Deung YK: Ultrastructure of fertilized egg envelop in the tomato clown anemonefish, Amphiprion freatus (Pomacentridae: Marine teleostei), Korean J Electron Microscopy 28 (3): 273-282, 1998b. (Korean)
  9. Kim DH, Deung YK, Kim WJ, Reu DS, Kang SJ: Comparative ultrastructures of the fertilized egg envelopes from three-spot gourami, pearl gourami and marble gourami, Belontiidae, Teleost, Korean J Electron Microscopy 29 (3): 343-351, 1999. (Korean)
  10. Kim DH, Deung YK, Kim HY, Reu DS: Ultrastructure of the fertilized egg envelope from long nose barbel, Cyprinidae, Teleost, Korean J Electron Microscopy 31 (1): 85-90, 2001. (Korean)
  11. Kim IS and Kang YJ: Coloured fishes of Korea, Academy Publishing Company: 373-374, 1993. (Korean)
  12. Kim YU, Myoung JG, Choi SO: Egg development and larvae of the hornfish, Hemiramphus sajori, Bull Korean Fish Soc 17 (2): 125-131, 1984. (Korean)
  13. Laale HW: The perivitelline space and egg envelopes of bony fishes: a review, Copeia 2: 210-226, 1980
  14. Lönning S: Comparative electron microscopic studies of teleostean eggs with special reference to the chorion, Sarsia 49: 41-48, 1972 https://doi.org/10.1080/00364827.1972.10411206