• Journal of Marine Bioscience and Biotechnology
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• v.6 no.2
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• pp.118-122
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• 2014

The ocean provide great benefits for microalgal mass cultures with maintaining stable temperature due to high specific heat, mixing by wave energy, and providing large area for large-scale microalgae cultures. In this study, we cultivated a marine green microalga, Tetraselmis sp. KCTC12432BP, using marine photobioreactors on the ocean for investigating the effect of $NaHCO_3$ concentration on the biomass productivities and evaluating the potential of ocean microalgae culture. The culture medium consist of three fold concentrated f/2-Si with 4 g/L of $NaHCO_3$, which is dissolved in natural seawater. After 11 days of cultivation, the cultures reached stationary phase at biomass concentration of 1.6 g/L. At that time, $NaHCO_3$ concentration of 0, 2, and 4 g/L were fed to the cultures. The daily productivities of 0.11, 0.19, 0.30 g/L/day were attained with feeding rate of 0, 2, and 4 g/L $NaHCO_3$, respectively. Biomass productivity of Tetraselmis sp. KCTC12432BP was a function of the $NaHCO_3$ feeding rate as expected. This research shows that the microalgae can grow with $NaHCO_3$ as carbon source in marine photobioreactors on the ocean while exploiting various benefits of ocean cultivation.

• Ocean and Polar Research
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• v.35 no.4
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• pp.383-394
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• 2013

The purpose of this study is to demonstrate, through case studies, the usefulness of utilizing local R&D centers under science and technology ODA programs. For the past few decades, advanced countries have supported ODA projects of developing countries, but there have been negative opinions regarding the results. Through a case study of the black pearl cultivation project between the Korea Institute of Ocean Science & Technology and Micronesia, this study explains the usefulness of actively utilizing Korean R&D centers established and operational in recipient countries. Although black pearl cultivation is not an ODA project, the case study offers valuable insights as it is operated in a similar form and thus highly applicable to future projects. Based on the case study, four implications were derived to ensure the successful operations of science and technology ODA projects in the future. First, there is a need to improve relevance by making use of the technological capacities of local R&D institutes to develop projects that reflect the needs of recipient and donor countries. Second, trust must be established with local communities over the long term in order to enhance the effectiveness and efficiency of project operations. Third, the proportion of science and technology ODA projects must be expanded to acquire sustainability, and more support should be granted to ODA projects involving marine resources, which are an advantage for countries of Micronesia. Lastly, the locals should be offered employment opportunities and regular training programs to allow for the actual transfer of knowledge instead of mere techniques. The implications derived in this study will prove useful in pursuing science and technology ODA projects, especially with Micronesia.

• Proceedings of KOSOMES biannual meeting
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• 2006.05a
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• pp.47-52
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• 2006

The cultural grounds of laver has been surveyed using SPOT-5 satellite images to calculate the facilities of laver cultivation area in the coastal waters of Korea 10m resolution multispectral images of SPOT-5 are adopted for the south area of Daebu Island, Hwaseong city to develop an automatic detection approach of laver nets that consists of the following: band difference technique, canny edge detector and morphological analysis. The satellite-based facilities number was relatively high as compared with the licensed number in 2005, 676,749 chaek and 572,745 chaek(柵, unit of measure for laver farm), respectively. The data could be applied to achieve a good harvest for laver seaweed growers and to control its national production keeping a stable market price for the government body.

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2001.10a
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• pp.23-24
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• 2001

Iro-ochi as has been called by Nori (Porphyra) farmers is biologically a bleaching phenomenon and has been one of the serious problems in Nori cultivation in Japan. Especially in the last Nori cultivation season, Iro-ochi gave an extremely serious damage to the Nori production in Ariake Bay, Kyushu, Japan, which attracted considerable socio-economic attention in Japan. As early as in late 1960s, we already made fundamental studies on Iro-ochi of Porphyra. (omitted)

• Proceedings of the KSRS Conference
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• 2007.03a
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• pp.167-170
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• 2007

연안 김 양식장의 효과적 관리를 위해서는 실제 시설량의 조사가 펼요하며 인공위성을 이용한 방법이 가장 효과적이다. 본 연구에서는 스펙트로미터에 의한 해수 및 김 양식장 시설에 대한 광 측정을 통하여 파장별 특성을 조사하였다 10m의 해상도를 갖고 있는 SPOT-5 다중분광영상을 사용하였으며，김 양식장의 자동탐지알고리듬의 개발을 위하여 경기도 화성시 제부도 남방해역에 대한 2005년도 영상을 사용하였다. 김 양식장을 추출하기 위하여 우선 3밴드 영상의 분광특성을 이용한 밴드차(Band difference) 영상을 작성하여，두 가지 방법(형태학적 처리기법 및 Canny 에지 탐지기법)으로 처리를 한 후，두 결과를 합성하여 라벨령함으로써 탐지율을 극대화하였다 양식장 시설 현황 조사 결과는，정부에서 전체 생산량을 조절할 수 있게 하며，양식업자가 좋은 수확을 달성하는데 도움이 될 수 있을 것이다.

• Journal of Microbiology and Biotechnology
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• v.17 no.1
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• pp.168-175
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• 2007

We report here on the cultivation of numerous novel bacterial species from a eutrophic freshwater pond. A total of 60 strains, 15 strains per each culture medium, were obtained from the surface of a eutrophic freshwater pond by employing a conventional dilution-plating method with four different kinds of culture media, including R2A, 1/10R2A, PCA, and 1/10PCA. Among the 60 strains isolated, 27 strains showed less than 97% 16S rRNA gene sequence similarities to validly published species, and thus they are considered to comprise 19 novel species. Of the 27 strains assigned to the novel species, the majority of the strains (20 strains) were affiliated with the Alphaproteobacteria and Betaproteobacteria. The remaining 7 strains were affiliated with the Gammaproteobacteria, Firmicutes, Actinobacteria, and Deinococci. Because we have isolated 19 novel species from a usual freshwater pond using a conventional culturing technique, our results suggest that an unexplored ecosystem, even if it looks like a common ecosystem found elsewhere, harbors diverse unidentified microbes, which will be definitely further characterized.

• Journal of Marine Bioscience and Biotechnology
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• v.11 no.1
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• pp.23-28
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• 2019

For successful microalgal biodiesel production, the strain should be selected carefully. Fast growth rate and high fatty acid contents are desired traits for algal biodiesel production. In ocean cultivation of microalgae, seawater temperature slowly changes over seasons, and rotating algal strains in accordance with their optimal temperature could improve overall productivity. Additionally, use of indigenous strain is preferred to alleviate potential impacts on the environment. In this study, five strains of Tetraselmis sp. from nearshore of Youngheung Island, Incheon, Korea, were isolated during winter and characterized for their growth patterns and fatty acid compositions in the low temperatures ($5-15^{\circ}C$). The five strains showed various characteristics in optimal growth temperature, fatty acid contents, and compositions. Compared with a strain of Tetraselmis sp., isolated from Ganghwa island in a previous study, a rapid-growing strain with 237% higher biomass productivity and an oleaginous strain with twice higher fatty acid contents at $10^{\circ}C$ were isolated. The oleaginous Tetraselmis strain showed the highest fatty acid productivity among the strains, having 438% higher productivity than the previous strain. Using the new isolates in the seasons with low seawater temperature would improve microalgal fatty acid productivity in ocean cultivation.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.20 no.3
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• pp.237-247
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• 1987

The present study deals with the physico-chemical and meteorological conditions in Porphyra-cultivation ground to determine the relationship between laver production and its environmental factors in Kwangyang Bay from January to April in 1986. As a result, major environmental factors which are thought to have a great influence upon the poor harvest during the cultivation period are as follows; 1) the excessive rainfall in the beginning of cultivation period 2) the accumulation of suspended matters on the thallus of laver 3) the decrease of current velocity and the stagnation of the water in the cultivation ground.

• Proceedings of the Korea Committee for Ocean Resources and Engineering Conference
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• 2003.10a
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• pp.329-332
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• 2003

Aquaculture have been important role to supply food resources for mankind. However, competitive power of domestic mariculture industry was declined due to increase of labor and feed expenditures, and quantity import of low-priced livefishes from the developing underdeveloped nations in North and South East Asia. Mass production and quality enhancement can be pointed out to overcome such an industrial environment in this decade. To meet these requirement, movable mariculture base remodeling feasible vessel of chemical tanker or crude oil carrier has been proposed for more advanced mariculture management system by using deep seawater from about 200m which is sustainablely clean, nutrient-rich and cold seawater. Deep seawater can be applied for control of seawater temperature for mariculture base and cultivation phytoplankton and seaweed as feed. Besides mariculture, strategic marketing can be implemented by raw water and ice of deep seawater. Feasibility of applying deep seawater was considered after evaluating general movable mariculture base and management system.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.20 no.4
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• pp.328-340
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• 1987

In order to clarify major reasons of a poor harvest, the Porphyra-cultivation ground was investigated in Kwangyang Bay in terms of the laver production per unit area, diseases, and fouling organisms from January to April in 1986. The laver production per unit area of the control station was more than that of the study area at 'Soppal' cultivation ground, while the difference at the cultivation groud using 'nets' seemed to be insignificant. The vertical attachment range of laver was directly associated with the production per unit area at 'Soppal' cultivation ground. The number of young thalli of 1-3 mm length was almost similar between control and study sites at 'nets' cultivation ground. Two kinds of diseases in cultured laver, the crape and shot hole, were prevalent during the study period. However, the affected thalli were gradually decreased at the end of the study period. Major fouling organisms attached to 'Soppal' were Balanus albicostatus, Enteromorpha prolifera, Capsosiphon fulvescens, and Scytosiphon lomentaria. In conclusion, the poor harvest of laver in 1986 may result from such following problems as the decrease in vertical attachment range of laver and fouling problems as the decrease in vertical attachment range of laver and fouling organisms at 'Soppal' cultivation ground, prevalence of certain diseases, changes in environmental conditions, arrangement and maintenance of facilities for laver cultivation.