• 원예과학기술지
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• 제34권1호
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• pp.102-111
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• 2016

본 연구의 목적은 BrDSR(Drought Stress Resistance in B. rapa) 유전자의 기능을 명확히 밝히고, 배추에서 건조 스트레스 반응 유전자들을 분석하는데 있다. 내혼계배추('CT001')와 BrDSR 완전장(438bp의 오픈리딩프레임)을 지닌 pSL100 vector를 재료로 아그로박테리아를 이용한 배추 형질전환을 수행하였다. PCR 분석을 통해 4개체의 형질전환체를 확보하였고, 이들의 BrDSR 발현량은 건조 스트레스 조건에서 비형질전환체 대비 약 1.9-3.4배 정도 더 큰 것으로 분석되었다. 또한 표현형 분석에서도 BrDSR이 과발현된 형질전환체들은 건조 스트레스에 저항성을 보이며 정상적인 생장을 하였다. 기 구축된 건조 스트레스 반응 유전자의 상호발현 네트워크를 기반으로 BrDSR과 밀접한 관련이 있는 유전자들을 분석하기 위해 B. rapa 135K cDNA microarray 데이터를 분석하였다. 그 결과, 환경 스트레스와 관련하여 식물체에서 잎의 노화와 자가소화에 관련된 것으로 보고된 'dark inducible 2(DIN2, AT3G60140)'와 'autophagy 8h(ATG8H, AT3G06420)' 유전자가 확인되었다. 위 결과들을 근거로 BrDSR 유전자는 건조 스트레스에 대한 저항성 향상에 중요한 역할을 할 것으로 판단되었다.

• 대한지역사회영양학회지
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• 제3권4호
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• pp.533-541
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• 1998

This study compares the growth performance(weight-for-age Z-score, height-for-age Z-score, weight-for-height Z-score) of one- to three- month-old Korean infants(n=232) with the Korean standard(1994) and NCHS reference(1983). The weight-for-age Z-scores(WAZ) by the Korean standard were -2∼1,5 for males and -3∼1 for females. The NCHS reference, WAZ results were -1∼2.5 and -2.5∼3.5 for males and females, respectively. The WAZ compared Korean standard showed no subjects with overnutrition. But 1.3% of the infants showed signs of malnutrition. Comparison with NCHS reference revealed that 0.4% of the infants were malnourished and 7.0% of the infants were overnourished. The WAZ of formula-fed infants were distributed higher than breast-fed infants, but that was not significant. The height-for-age Z-score(HAZ) by the Korean standard were -4.5%∼1.5% for males and -4∼1.5% for females. According to the NCHS reference, HAZ were -2.5%∼2.5% and -3∼3 for males and females, respectively. When the WAZ was compared with the Korean standard, there was no overnutrition but 16% of the infants showed signs of malnutrition. The NCHS reference, revealed that 6.9% of the infants were malnourished and 1.8% of the infants were ovemourished. The weight-for-height Z-scores(WHZ) by the Korean standard were -2∼5 for males and -2.5∼5 for females. The NCHS reference WAZ scores were -1∼4 and -1.5∼4.5 for males and females, respectively. When the WHZ was compared with the Korean standard, 2.2% of infants were malnourished and 19.5% were overnourished. There were no malnourished subjects according to the NCHS reference and 19.1% of the infants were overnourished. When the three Z-scores are considered together, 92.0% of the infants should a normal growth status, there was no malnutrition, and 8.0% of the infants were overnourished. The growth performance was evaluated differently according to the type of standards. Thus, it is necessary to set proper growth standards for infants, according to which classification of feeding methods is chosen. A longterm and careful assessment of infants's growth performances to develop any group of standards.

• 대한지역사회영양학회지
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• 제8권2호
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• pp.181-191
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• 2003

The purpose of this study was to investigate the dietary habits, the nutritional knowledge and the consumption patterns of convenience foods of 618 university students located in the Gwangju area, from November twentieth to November thirtieth, 2002. The subjects included 301 males (48.7%) and 317 females (51.3%). This survey was conducted using a self-administered questionnaire. The percentage of students who were underweight, normal and overweight based on their body mass index (BMI) was 3.7, 88.0 and 8.3 far the males, and 29, 71 and 0 for the females, respectively. Most students (73.8%) skipped breakfast. This was because of lack of time (65.1%) of the cases. Self-reported eating habit problems were eating irregular meal (52.4%), overeating (21%), prejudiced meal (12.4%) and skipping meal (7.4%). The type of favorite snack was biscuit (31.9%) and cup Ramyon (31.9%). The rates of alcohol drinking in male and female students were 88.0% and 85.8% respectively, and the rates of smoking were 35.9% and 28% respectively. Males ate fiequently cup Ramyon (2.08 $\pm$ 2.95), fries (pork cutlet, fried potato) (1.91 $\pm$ 2.58), Ramyon (1.81 $\pm$ 3.00) and kimbab (1.70 $\pm$ 3.17) more frequently, while females ate kimbab (2.25 $\pm$ 3.26), cup Ramyon (1.89 $\pm$ 2.09), fries (pork cutlet, fried potato) (1.77 $\pm$ 2.67), Ramyon (1.46 $\pm$ 1.1.95) and fish paste (1.45 $\pm$ 2.22) more frequently. The nutritional knowledge score was higher in females than in males. The mean consumption patterns of convenience foods of students living in home were lower than that of students living in dormitory and boarding w/cooking. When the amount of pocket money available and the body mass index increased and the nutritional knowledge level was low, the mean consumption of convenience foods increased. The mean consumption of convenience foods correlated negatively with nutritional knowledge (p < 0.01). Therefore, nutritional education for university students is needed so as to improve their health and nutritional education program should be developed to meet the various needs of these students. (Korean J Community Nutrition 8(2) : 181~191, 2003)

• 한국멀티미디어학회논문지
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• 제16권1호
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• pp.100-107
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• 2013

최근 전자책 시장이 활성화됨에 따라 전자책 단말기에서부터 스마트 기기의 소프트웨어 리더까지 각종 전자책뷰어가 등장하고 있다. 하지만 시각장애인, 난독증, 학습장애인과 같은 독서장애인을 위한 모바일 전자책 인터페이스에 대한 개발과 연구는 부족한 실정이다. 비장애인을 대상으로 만들어진 전자책뷰어는 독서장애인에게 그대로 적용할 수 없기 때문에 독서장애 사용자의 특성에 따라 차별화된 인터페이스가 요구된다. 이에 본 논문에서는 독서장애인용 전자책 표준 형식을 지원하는 모바일 전자책 뷰어 인터페이스 모델을 제안한다. 제시 모델은 전맹인, 저시력인, 학습장애인 등 사용자의 특성 및 상황(context)에 따라 차별화된 인터페이스를 제공한다. 아울러 독서장애인용 어노테이션 시스템을 지원함으로써 기존의 독서장애인용 오디오북과는 다른 사용자-전자책 간의 상호작용을 지원한다. 또한 본 모델을 이용하여 스마트폰 플랫폼인 안드로이드 환경에서의 독서장애인용 전자책뷰어 프로토타입을 구현하고 그 활용 가능성을 제시한다. 본 연구의 결과는 국내 인구 10%에 해당하는 독서장애인의 효율적인 독서활동을 지원할 수 있다.

• Nutrition Research and Practice
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• 제10권1호
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• pp.11-18
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• 2016

BACKGROUND/OBJECTIVES: Type 2 diabetes (T2D) is more frequently diagnosed and is characterized by hyperglycemia and insulin resistance. $\small{D}$-xylose, a sucrase inhibitor, may be useful as a functional sugar complement to inhibit increases in blood glucose levels. The objective of this study was to investigate the anti-diabetic effects of $\small{D}$-xylose both in vitro and stretpozotocin (STZ)-nicotinamide (NA)-induced models in vivo. MATERIALS/METHODS: Wistar rats were divided into the following groups: (i) normal control; (ii) diabetic control; (iii) diabetic rats supplemented with a diet where 5% of the total sucrose content in the diet was replaced with $\small{D}$-xylose; and (iv) diabetic rats supplemented with a diet where 10% of the total sucrose content in the diet was replaced with $\small{D}$-xylose. These groups were maintained for two weeks. The effects of $\small{D}$-xylose on blood glucose levels were examined using oral glucose tolerance test, insulin secretion assays, histology of liver and pancreas tissues, and analysis of phosphoenolpyruvate carboxylase (PEPCK) expression in liver tissues of a STZ-NA-induced experimental rat model. Levels of glucose uptake and insulin secretion by differentiated C2C12 muscle cells and INS-1 pancreatic ${\beta}$-cells were analyzed. RESULTS: In vivo, $\small{D}$-xylose supplementation significantly reduced fasting serum glucose levels (P < 0.05), it slightly reduced the area under the glucose curve, and increased insulin levels compared to the diabetic controls. $\small{D}$-xylose supplementation enhanced the regeneration of pancreas tissue and improved the arrangement of hepatocytes compared to the diabetic controls. Lower levels of PEPCK were detected in the liver tissues of $\small{D}$-xylose-supplemented rats (P < 0.05). In vitro, both 2-NBDG uptake by C2C12 cells and insulin secretion by INS-1 cells were increased with $\small{D}$-xylose supplementation in a dose-dependent manner compared to treatment with glucose alone. CONCLUSIONS: In this study, $\small{D}$-xylose exerted anti-diabetic effects in vivo by regulating blood glucose levels via regeneration of damaged pancreas and liver tissues and regulation of PEPCK, a key rate-limiting enzyme in the process of gluconeogenesis. In vitro, $\small{D}$-xylose induced the uptake of glucose by muscle cells and the secretion of insulin cells by ${\beta}$-cells. These mechanistic insights will facilitate the development of highly effective strategy for T2D.

• Journal of Nutrition and Health
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• 제44권1호
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• pp.16-28
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• 2011

Oxidative stress leads to the induction of cellular oxidative damage, which may cause adverse modifications of DNA, proteins, and lipids. The production of reactive species during oxidative stress contributes to the pathogenesis of many diseases. Antioxidant defenses can neutralize reactive oxygen species and protect against oxidative damage. The aim of this study was to assess the antioxidant status and the degree of DNA damage in Korean young adults using glutathione s-transferase (GST) polymorphisms. The GSTM1 and GSTT1 genotypes were characterized in 245 healthy young adults by smoking status, and their oxidative DNA damage in lymphocytes and antioxidant status were assessed by GST genotype. General characteristics were investigated by simple questionnaire. From the blood of the subjects, GST genotypes; degree of DNA damage in lymphocytes; the erythrocyte activities of superoxide dismutase, catalase, and glutathione peroxidase; plasma concentrations of total peroxyl radical-trapping potential (TRAP), vitamin C, ${\alpha}$- and ${\gamma}$-tocopherol, ${\alpha}$- and ${\beta}$-carotene and cryptoxanthin, as well as plasma lipid profiles, conjugated diene (CD), GOT, and GPT were analyzed. Of the 245 subjects studied, 23.2% were GSTM1 wild genotypes and 33.4% were GSTT1 wild genotype. No difference in erythrocyte activities of superoxide dismutase, catalase, or glutathione peroxidase, and the plasma TRAP level, CD, GOT, and GPT levels were observed between smokers and non-smokers categorized by GSTM1 or GSTT1 genotype. Plasma levels of ${\alpha}$- and ${\gamma}$-tocopherol increased significantly in smokers with the GSTT1 wild genotype (p < 0.05); however, plasma level of ${\alpha}$-carotene decreased significantly in non-smokers with the GSTM1 wild genotype (p < 0.05). DNA damage assessed by the Comet assay was significantly higher in non-smokers with the GSTM1 genotype; whereas DNA damage was significantly lower in non-smokers with the GSTT1 genotype. Total cholesterol and LDL cholesterol levels were significantly higher in non-smokers with the GSTT1 genotype than those with the GSTT1 wild genotype (p < 0.05). In conclusion, the GSTM1 genotype or the GSTT1 wild genotype in non-smokers aggravated their antioxidant status through DNA damage of lymphocytes; however, the GSTT1 wild type in non-smokers had normal plasma total cholesterol and LDL-cholesterol levels. This finding confirms that GST polymorphisms could be an important determinant of antioxidant status and plasma lipid profiles in non-smoking young adults. Further study is necessary to clarify the antioxidant status and/or lipid profiles of smokers with the GST polymorphism and to conduct a study with significantly more subjects.

• 과학기술학연구
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• 제19권3호
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• pp.201-246
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• 2019

정보통신기술은 교육을 큰 위기에 빠트리는 위험물질 취급을 받기도 하고, 위기에서 구원하는 구원자로 비추어지기도 한다. 한국 정부는 2011년 스마트교육 정책을 통해 정보통신기술을 적극 공교육의 학교들로 끌어들여 교육을 혁신하고 인재대국을 건설하겠다고 발표했다. 스마트교육은 타블렛 PC나 노트북과 같은 기기를 활용하여 수업을 진행할 수 있는 표준 교실 모델을 정립해 각 학교에 확산, 정착시키고자 했다. 이 논문은 스마트교육 정책이 꿈꾸었던 스마트교실이 실제 교육 현장에서는 어떤 마찰을 일으켰는지 분석한다. 이를 위해 스마트교육 정책의 방향과 이상을 분석하고, 실제로 스마트한 교실 환경을 구축하고 있는 학교 세 곳에 방문해 시설 견학 및 심층 인터뷰를 수행했다. 스마트교육 정책이 제안한 이상적 교실은 모델하우스처럼 깔끔한 공간에 스마트폰처럼 다양한 기능을 한 번에 갖춘 패키지였지만 일상적으로 보통 교실처럼 활용되기 어려웠다. 각 학교는 모델을 수용하는 대신 규모, 지역 특성, 학생들과 교사의 사회경제적 상황, 교사의 경험, 기존 인프라의 유무 등의 요인들이 조합되는 방식에 따라 각자의 방식으로 "스마트"해졌다. A 초등학교에서는 기계가 학교의 공간을 길들여 자신만의 공간을 만들어내는 한편 이동을 위해서는 학교의 규칙에 따르게 되었다. B 초등학교에서는 학교 전반을 아우르는 인프라가 만들어지지는 못했지만 선생님의 경험에 힘입어 조직화했으며, C 초등학교에서는 특정 교실에 밀집되어 선생님의 전문성을 증진시켰다. 스마트교실의 구성하고 있던 기기들은 분해되고, 맥락에 맞게 재조합되어 각 학교의 교실들을 "스마트"하게 만들었다.

• 여성건강간호학회지
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• 제4권1호
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• pp.84-102
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• 1998

In this study the levels and influencing characteristics of maternal identity and self confidence for caring the baby were identified during the immediate postpartum period. 114 primiparous women who delivered vaginally normal baby participated in the survey from August 1 to October 31, 1996. SD scale was to measure maternal identity which consisted of 11 items for mother and 6 items for baby. Likert scale was to measure self confidence for caring the baby (38 items). Cronbach's alphas for evaluating internal consistency as follows : .86 for maternal identity and .96 for self confidence scale for caring the baby. The study showed these results : 1. Mean score of maternal identity(82.03 : 52.65 for mother, 29.38 for baby) was considered relatively low. 2. Mean score of self confidence for caring the baby(113.91) was considered relatively low. 3. There were significant influencing characteristics of primiparous to maternal identity : age(total ; F=3.53, p=.0329, for mother ; F=2.60, p=.0719, for baby ; F=3.12, p=.0481), prenatal infant care preparation(total ; t=2.31, p=.0306, for mother ; F=2.62, p=.0160), knowledge about infant care during the hospitalization(for baby ; F=3.94, p=.02222), colostrum feeding(total ; t=1.95, p=.0541, for baby ; t=2.71, p=.0080), frequency of breast feeding during the hospitalization(for baby ; F=3.91, p=.0228)and feeding type after discharge(for baby ; F=3.18, p=.0456). 4. There were significant influencing characteristics of primiparous to self confidence for caring the baby : routine husband support(F=6.09, p=.0031), prenatal infant care preparation(t=2.04, p=.0574), knowledge about infant care during the hospitalization(F=3.15, p=.0467), education of breast feeding during the hospitalization(t=-1.79, p=.0850). 5. Correlation between maternal identity and self confidence for caring the baby was r=.37608(p=.0001). This study implies that special education programs for primipara are needed. For the future, this study suggest that maternal role variables need to be monitored through the home visiting follow up. Also intervention programs related to prenatal care, discharge education, home visiting follow up need to be developed and then evaluated their effectiveness.

• 한국건설순환자원학회논문집
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• 제7권3호
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• pp.194-201
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• 2019

본 연구는 CGS를 콘크리트용 잔골재로서 활용성을 검토하기 위하여 CGS 치환율에 따른 콘크리트의 특성을 분석하였다. 실험결과, 슬럼프 및 슬럼프 플로우는 CSa 및 MS 모두 CGS 치환율이 증가할수록 증가하였으며, 공기량은 반대로 감소하였다. 재료분리평가정수(EISN)는 CSa를 사용한 경우 CGS 25%, MS를 사용한 경우는 CGS 50~75%에서 양호한 것으로 나타났다. 압축강도는 CSa를 사용한 경우 CGS 치환율이 증가할수록 저하하였는데, CGS 25%에서 저하폭이 가장 작았다. 단, MS를 사용한 경우는 포물선 경향으로 CGS 50%에서 최대치를 나타내었다. 상호비교로서 잔골재 종류에 따른 압축강도는 MS보다 CSa가 약 8% 높게 나타났고, CGS 사용 유무에 따라서는 CGS를 사용하였을 때 낮은 강도 영역에서는 작게 나타났지만, 높은 강도 영역에서 크게 나타났다. CGS 치환율에 따른 콘크리트의 품질을 종합적으로 검토한 결과, CGS를 잔골재에 25~50% 전후로 혼합하여 사용한다면 유동성 및 강도 증진에 긍정적으로 기여할 수 있을 것으로 판단되었다.

• 대한화장품학회지
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• 제41권4호
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• pp.351-359
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• 2015

극심하고 지속적인 자외선에의 노출은 정상적인 피부구조를 파괴하는 다양한 피부 광노화 과정을 야기한다. 자외선은 인체 피부에서 세포외기질의 구성성분을 분해시키는 기질 분해효소인 matrix metalloproteinases(MMPs)의 발현을 활성화시키고, 콜라겐 합성은 감소시킴으로써 피부의 탄력과 구조적 치밀도를 약화시켜 궁극적으로 피부주름을 생성한다. 본 연구에서는 이러한 피부 광노화 현상을 완화시키는 소재로서 영실의 효능을 검증하고자 하였다. 먼저 인간 섬유아세포주인 Hs68을 이용하여 영실의 세포증식 촉진효능을 확인하였다. 여기에 더해 영실이 activator protein (AP)-1 전사인자의 억제를 통해 MMP의 발현을 감소시킴을 mRNA 및 단백질 수준에서 검증하였다. 또한, 진피층을 구성하는 타입 I형 콜라겐과 표피-진피 경계부를 단단히 고정시키는 역할을 하는 타입 IV형 콜라겐 역시 영실에 의해 발현이 증가하며, 자외선에 의한 염증반응의 억제에도 영실이 효과적으로 작용하는 것을 확인할 수 있었다. 결론적으로 본 연구를 통해 영실이 자외선에 의한 피부노화와 주름생성을 효과적으로 개선할 수 있는 가능성을 가짐으로써 항노화, 항염증 및 항주름 소재로서 화장품에 응용될 수 있을 것으로 기대된다.