• BMB Reports
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• 제43권5호
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• pp.305-310
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• 2010

Syndecans, cell surface heparansulfate proteoglycans, have been proposed to act as cell surface receptors and/or coreceptors to play critical roles in multiple cellular functions. However, recent reports suggest that the function of syndecans can be further extended through shedding, a cleavage of extracellular domain. Shedding constitutes an additional level for controlling the function of syndecans, providing a means to attenuate and/or regulate amplitude and duration of syndecan signals by modulating the activity of syndecans as cell surface receptors. Whether these remaining cleavage products are still capable of functioning as cell surface receptors to efficiently transduce signals inside of cells is not clear. However, shedding transforms cell surface receptor syndecans into soluble forms, which, like growth factors, may act as novel ligands to induce cellular responses by association with other cell surface receptors. It is becoming interestingly evident that shed syndecans also contribute significantly to syndecan functions in cancer biology. This review presents current knowledge about syndecan shedding and its functional significance, particularly in the context of cancer.

• Biomolecules & Therapeutics
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• 제19권1호
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• pp.1-8
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• 2011

G-protein coupled receptors (GPCRs) constitute an important class of drug targets and are involved in every aspect of human physiology including sleep regulation, blood pressure, mood, food intake, perception of pain, control of cancer growth, and immune response. Radiometric assays have been the classic method used during the search for potential therapeutics acting at various GPCRs for most GPCR-based drug discovery research programs. An increasing number of diverse small molecules, together with novel GPCR targets identified from genomics efforts, necessitates the use of high-throughput assays with a good sensitivity and specificity. Currently, a wide array of high-throughput tools for research on GPCRs is available and can be used to study receptor-ligand interaction, receptor driven functional response, receptor-receptor interaction,and receptor internalization. Many of the assay technologies are based on luminescence or fluorescence and can be easily applied in cell based models to reduce gaps between in vitro and in vivo studies for drug discovery processes. Especially, cell based models for GPCR can be efficiently employed to deconvolute the integrated information concerning the ligand-receptor-function axis obtained from label-free detection technology. This review covers various platform technologies used for the research of GPCRs, concentrating on the principal, non-radiometric homogeneous assay technologies. As current technology is rapidly advancing, the combination of probe chemistry, optical instruments, and GPCR biology will provide us with many new technologies to apply in the future.

• Mass Spectrometry Letters
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• 제1권1호
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• pp.13-16
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• 2010

The active components in a plant extract can be represented as mass profiles. We introduce here a new, multi-compound discovery method known as Scaling of Correlations between Activity and Mass Profiles (SCAMP). In this method, a correlation coefficient is used to quantify similarities between the extract activity and mass profiles. The method was evaluated by first measuring the anti-oxidation activity of eleven fractions of an Astragali Radix extract using DPPH assays. Next, 15 T Fouriertransform ion cyclotron resonance (FT-ICR) MS was employed to generate mass profiles of the eleven fractions. A comparison of correlation coefficients indicated two compounds at m/z 285.076 and 286.076 that were strong antioxidants. Principal component analyses of these profiles yielded the same result. FT-ICR MS, which offers a mass resolving power of 500,000, was used to discern isotopic fine structures and indicated that the molecular formula corresponding to the peak at m/z 285.076 was $C_{16}H_{13}O_5$. SCAMP in combination with high-resolution MS can be applied to any type of mixture to study pharmacological activity and is a powerful tool for active compound discovery in plant extract studies.

• ETRI Journal
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• 제27권4호
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• pp.355-366
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• 2005

Automatic discovery of physical topology plays a crucial role in enhancing the manageability of modern metro Ethernet networks. Despite the importance of the problem, earlier research and commercial network management tools have typically concentrated on either discovering logical topology, or proprietary solutions targeting specific product families. Recent works have demonstrated that network topology can be determined using the standard simple network management protocol (SNMP) management information base (MIB), but these algorithms depend on address forwarding table (AFT) entries and can find only spanning tree paths in an Ethernet mesh network. A previous work by Breibart et al. requires that AFT entries be complete; however, that can be a risky assumption in a realistic Ethernet mesh network. In this paper, we have proposed a new physical topology discovery algorithm which works without complete knowledge of AFT entries. Our algorithm can discover a complete physical topology including inactive interfaces eliminated by the spanning tree protocol in metro Ethernet networks. The effectiveness of the algorithm is demonstrated by implementation.

• 한국컴퓨터정보학회논문지
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• 제17권2호
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• pp.139-147
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• 2012

본 논문에서는 텍스트 마이닝을 통해 생물학 문헌에서 분자 수준의 사건(event) 정보를 자동으로 추출하고, 이들 사건 정보를 기반으로 새로운 생물학 지식을 자동 추론하는 텍스트 마이닝 - 추론 통합 구조의 시스템을 다룬다. 이러한 통합 구조의 지식 발견 시스템은 미리 추출되어 데이터베이스에 등록된 정보만을 입력으로 사용하는 시스템들에 비하여 최신 정보를 보다 빨리 사용할 수 있고, 미리 정의된 형식 이외의 다양한 정보를 사용할 수 있다는 장점이 있다. 반면, 텍스트 마이닝 정보 추출 결과를 그대로 사용하기 때문에 텍스트 마이닝 모듈(module)의 성능에 따라 전체 시스템의 효용성이 크게 저하될 수도 있다는 문제가 있다. 본 논문에서는 확률 기반 필터링(filtering) 방법을 제안하여, 텍스트 마이닝 결과 중 양성 오류(false positive)를 효과적으로 제거함으로써 전체 지식 발견 시스템의 정확도 및 효용성을 높이고자 한다. 본 논문에서 제안한 확률 기반 필터링 방법은 기준(baseline) 방법으로 사용된 횟수 기반 필터링 방법보다 높은 성능을 보였다.

• 한국콘텐츠학회논문지
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• 제21권6호
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• pp.425-434
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• 2021

본 연구는 머레이 쉐이퍼(Murray Schafer)의 사운드스케이프(Soundscape) 개념을 통해 소리의 구분, 지각, 요소의 이해를 바탕으로 일상생활 속의 소음과 비음악적인 소리를 활용한 무용작품 의 창작과정과 이에 대한 음악적인 효과 및 새로운 안무 유형의 가치를 탐구하는 것을 연구의 목적으로 두었다. 실기기반연구(Practice-based Research)의 방법론적 연구에 따라 실기, 이론, 평가의 단계를 몸적 자료(Somatic Data)로 축적하였으며, 『공간디자인과 조형연습』(2003)의 일부분을 기반으로 형상화 방법, 움직임 형식, 공간화 방식으로 제시하여 분석의 근거를 마련하였다. 그 결과 창작과정에서 사운드스케이프, 즉 일상생활 속의 소리에 대한 음악적 효과 및 새로운 움직임 창작방법을 발견해낼 수 있었으며, 소리 역시 작품의 전반적인 분위기, 움직임의 의미, 작품의 주제를 효과적으로 전달할 수 있는 가능성을 탐구하였다. 아울러 사운드 스케이프라는 개념을 바탕으로 연구되어 나타난 소리에 대한 잠재적 가능성은 무용작품의 또 다른 창작환경을 만들어갈 수 있을 것이라 기대한다.

• 환경생물
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• 제35권3호
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• pp.319-328
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• 2017

The continuous exploration in deep seawater from Korea makes us lead the discovery of ancient Chlorophyta, Palmophyllum, in the Korean coast. The phylogenetic analyses of 18S rRNA and rbcL genes demonstrate that our specimens are Palmophyllum crassum (Naccari) Rabenhorst, recorded in Japan and clearly distinguished from P. umbracola from New Zealand and California, USA. Palmophyllum crassum grows in the subtidal region, 8-30 m deep, and has a crustose thallus which is closely adherent to substrates such as non-geniculate crustose coralline algae, sponge, shells, or rocks. P. crassum is composed of numerous spherical cells embedded in the gelatinous matrix. The discovery of this ancient green seaweed implies that the Korean coast is one of the hotspots of algal species diversity and has the suitable marine environment for algal speciation. We suggest the grounds to conserve the Korean coast environmentally as the biodiversity center of marine species by studying the phylogeny of seaweeds.

• 한국IT서비스학회지
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• 제11권2호
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• pp.185-196
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• 2012

The rapid evolution of ICT industry brings not only new services or products but also changes from common individual life to whole human society. Coping with these situations and survival, companies cannot help regarding R&D as the most important thing. So, discovering R&D projects which are suitable for the alternation is a big issue for many companies. To resolve the issue, KT has adopted Innovative Management Methodology developed by Strategos, which is co-founded by Gary Harmel. This paper describes this Innovative Management Methodology tailored to KT R&D. The methodology consists of five phases : focusing discovery, discovery, ideas/domains, domain elaboration & aiming point and R&D project proposal. Also, it shows some interim findings that came from the Innovative Management Process. Finally, the future plan for elaborating the methodology itself and generating new R&D projects is suggested.

• Biomolecules & Therapeutics
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• 제28권3호
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• pp.222-229
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• 2020

The process of drug discovery and drug development consumes billions of dollars to bring a new drug to the market. Drug development is time consuming and sometimes, the failure rates are high. Thus, the pharmaceutical industry is looking for a better option for new drug discovery. Drug repositioning is a good alternative technology that has demonstrated many advantages over de novo drug development, the most important one being shorter drug development timelines. In the last two decades, drug repositioning has made tremendous impact on drug development technologies. In this review, we focus on the recent advances in drug repositioning technologies and discuss the repositioned drugs used for inflammatory diseases such as sepsis, asthma, and atopic dermatitis.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 1997년도 춘계학술대회
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• pp.3-5
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• 1997

Properties of a good drug include safety, efficacy, half-life and bioavailability. With the current approach to drug discovery based on receptor-based and cell-based screening methods, compounds are frequently moved into development with poor bioavailability. With low bioavailability, drug administration is typically limited to parenteral routes, thus limiting the potential wide-spread utility of these therapeutic agents. The first and most important factor limiting a drug's bioavailability is the intestinal membrane permeability which in turn determines the maximum fi:action of the dose administered that can be absorbed. We have recently utilized new intubation methods for performing permeability measurements in humans and establishing a fundamental human data base for correlating intestinal jejunal membrane permeabilities with permeabilities determined in other systems, e.g., animals, tissue culture, as well as physical chemical properties.