• 대한화장품학회지
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• 제38권4호
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• pp.305-310
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• 2012

우리는 평생 동안 하루도 빠짐없이 치약과 같은 구강관리 제품들을 사용한다. 이와 같이 매일 입에 사용되는 제품의 안전성이 담보되어야 함은 매우 중요한 일이다. 이전까지 이루어진 동물시험이나 임상시험에서 치약 내 계면활성제 등에 의한 구강 자극이 유발될 수 있음이 알려져 있다. 하지만, 동물복지를 위하여 유럽 화장품 법안은 화장품과 그에 사용하는 원료에 대한 동물 시험을 금지했다. 그로 인해 여러 분야에서 동물을 대체하거나 동물의 사용을 줄일 수 있는 동물대체 시험법의 개발이 활발하게 이루어지고 있다. 하지만, 현재까지 구강 점막 독성을 측정할 수 있는 방법으로 임상시험과 동물시험이 있었으며, 최근에는 구강 점막 조직 모델이나 구강 세포들을 활용한 방법들이 연구되고 있다. 이번 연구의 목적은 구강관리 제품의 안전성을 확보할 수 있는 동물대체 시험법을 개발하는 것이다. 구강 세포주(YD-38 cell)를 활용해 불용성 물질을 포함한 치약에 대한 시험이 가능하도록 구강 점막 자극 시험법을 개발하였고, 이 시험법으로 이전에 이루어진 동물시험에서 자극유발원으로 알려진 물질에 의한 자극을 구별해낼 수 있었다. 또한, 유아와 어린이 치약의 자극 수준이 일반 성인 치약에 비해 낮음을 증명하였다. 이 결과를 바탕으로 동물을 사용하지 않고 인체에 대한 위해성을 줄일 수 있도록, 구강관리 제품의 구강 점막 자극 수준을 평가할 수 있는 이 시험법이 하나의 새로운 구강 자극 시험 방법으로 사용될 수 있으리라 사료된다.

• KSBB Journal
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• 제11권3호
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• pp.329-339
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• 1996

The effects of ammonium ion on cell growth kinetics, monoclonal antibody productivity, and cell metabolism of hybridoma cells were investigated. The mouse-mouse hybridoma cell line VlIIH-8 producing mouse IgG2a was used as a model system. Ammonium ion showed an inhibitory effect on cell growth and monoclonal antibody production. New immobilized adsorbents were developed for the reduction of the inhibitory effect of ammonium ion. The ammonium ion selective zeolite, Phillipsite-Gismondine was entrapped in calcium alginate bead or in dialysis membrane and applied to the hybridoma cell culture system for the in situ removal of ammonium ion from culture media. The effects of ammonium the both serum supplemented and serum free media on the cell growth were studied by applying immobilized adsorbents of calcium alginate bead type. The results demonstrated a substantial enhancement in cell growth. Applying immobilized adsorbents of dialysis membrane type to serum supplemented media also resulted in the stimulation of cell growth, cell viability and monoclonal antibody production.

• 대한의생명과학회지
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• 제13권4호
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• pp.273-278
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• 2007

Stem cells have been the subject of increasing scientific interest because of their utility in numerous biomedical applications. Stem cells are capable of renewing themselves; that is, they can be continuously cultured in an undifferentiated state, giving rise to more specialized cells of the human body. Therefore, stem cells are an important new tools for developing unique, in vitro model systems to test drugs and chemicals and a potential to predict or anticipate toxicity in humans. In the present study, in vitro cultured F3 immortalized human neural stem cell line and in vivo adult Sprague Dawley rats was used to evaluate the cytotoxicity of anticancer drug paclitaxel. In vitro apoptotic activity of paclitaxel was evaluated in F3 cell line by a MTT assay and DAPI test. The cell death was induced with the treatment of 20 nM paclitaxel and chromatin degradation was detected by DAPI staining, which was analyzed by fluorescent microscope. In vivo studies, we also observed nestin immunoreactivity on subventricular zone, which is stem cell rich region in the adult brain of the SD rat. Immunofluorescent staining result shows that pixel intensities of nestin were decreased in a dose dependent manner. These results suggest that paclitaxel is able to induce cytotoxic activity both in F3 neural stem cell line and neural stem cell in SD rat brain.

• 대한전자공학회논문지SD
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• 제40권4호
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• pp.74-84
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• 2003

기존의 테스트 알고리즘은 대부분 셀간의 고장에 중심이 맞추어져 있어 메모리의 집적도의 증가와 더불어 일어나는 word-line 과 bit-line 결합 잡음에 의한 고장을 효과적으로 테스트 할 수 없다 본 논문에서는 word-line 결합 capacitance에 의한 고장의 가능성을 제시하고 새로운 고장 모델인 WLSFs(Word-Line Sensitive Faults)을 제안하였다. 또한 word-line 과 bit-line 결합 잡음을 동시에 고려한 알고리즘을 제시하여 고장의 확률을 높였고 고장의 원인을 기존의 고장 모델로는 되지 않음을 보여준다. 제안된 알고리즘은 기존의 기본적인 고장인 고착고장, 천이고장, 그리고 결합고장을 5개의 이웃셀 내에서 모두 검출할 수 있음을 보여준다.

• 한국동물학회지
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• 제38권2호
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• pp.204-211
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• 1995

In an effort to develop a new therapeutic strategy for human gene therapy of solid ovarian tumor, we studied the expression of the p53 tumor suppressor Sene as well as regulatory subunits of cyclic AMP (cAMP)-dependent protein kinase in human ovarian carcinoma cells. Four cell lines (2774, Caov-3, SK-OV-3 and OVCAR-3) were selected for the analyses. The p53 transcript and protein were detected only in the 2774 cell line by Northern and Western Bnalysis. In the relatively fast growing cell line, SK-OV-3, the %rope 1 a regulstorv subunit (RIA of CAMP-dependent protein kinase was the highest among the four cell lines. The expression level of $RII\beta$ protein was low in the four cell lines examined. These results maw point to a direction to select the target gene(sl to be employed for gene therapy to control the ovarian cancer.

• KIEE International Transactions on Electrophysics and Applications
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• 제4C권4호
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• pp.160-164
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• 2004

In this study, we demonstrate for the first time a bio-inspired Cell-Microsystem to manipulate and detect living cells. Cultured retinal pigment epithelial cell line (ARPE-19) was directed to grow in a pre-defined Cell-Microsystem. The three-dimensional micropillars of 5 ${\mu}{\textrm}{m}$ in height and diameter of the Cell-Microsystem were fabricated. Inhibited DNA synthesis and transformed cell morphology were observed throughout the culture period. The demonstration of manipulating and detecting living cells by the surface topography is a new approach, and it will be very useful for the future design of cell-based biosensors and bioactuators.

• 한국전자파학회논문지
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• 제19권1호
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• pp.40-45
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• 2008

본 논문에서는 개방 스터브를 갖는 평행 결합 선로의 해석과 이를 이용하여 고조파 저지 특성을 갖는 소형화된 새로운 브랜치 라인 하이브리드를 제안한다. 먼저, 개방 스터브를 포함한 평행 결합 선로 전체의 Z-파라미터를 유도하였으며, 이는 넓은 저지 대역을 갖는 단위 저역 통과 여파기(LUC)의 특성을 갖는다. 이를 브랜치 라인 하이브리드의 설계에 적용하여, 중심 주파수 1.8 GHz 근처에서는 기존 브랜치 라인 하이브리드와 동일한 특성을 가지지만 크기는 약 1/2로 줄이고, 5체배 고조파를 포함한 12 GHz까지 스퓨리어스 성분을 -20dB 이하로 저지하는 새로운 브랜치 라인 하이브리드를 설계하였다.

• 대한한방내과학회지
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• 제21권4호
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• pp.621-631
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• 2000

Objective : In this study, the medicine herbs above were directly treated to cultured normal lung cells and lung carcinoma cells, and the effects were investigated to develope new cancer treatments with increased anti-cancer efficiency as well as decreased side-effects and to suggest more useful clinical therapies. Materials and Methods : In the experiment, WI-26 VA lung normal cell line and A-427 lung carcinoma cell line were cultured. To observe the morphological change of the treated cells, were subjected to Giemsa staining and observed under Reflected Fluorescence microscope. To examine whether cell death occurred, cells observed under Reflected Fluorescence microscope, To investigate the degree of cell death in the nucleus, cells were screened by Laser cytometry ACAS 570. Results : Samgibopye-tang(Shenqibufei-tang) stimulated not only the growth of the normal cells but also that of the carcinoma cells, Wikyeung-tang(Weijing-tang) induced morphological change such as cytoplasmic constriction in the normal cells and the carcinoma cells, but it did not show any strong inhibitory effect on the cell growth. Samso-eum(Shensu-yin) caused severe cell damage in both cell lines, Eunkyo San(Yinqiao-san) significantly damaged the nuclei and caused weak cytoplasmic constriction in both cell lines, Normal cells treated with Gilgyeung-tang(Jingeng-tang) did not show any significant morphological change while some Gilgyeung-tang(Jingeng-tang) treated carcinoma cells were observed to have a normal cell-like shape, interestingly, conclusions : As the results above, Samgibopye-tang(Shenqibufei-tang), Wikyeung-tang(Weijing-tang), and Gilgyeung-tang(Jingeng-tang) helped the growth of both cell lines, and especially Samgibopye-tang(Shenqibufei-tang) showed the best effect, However, Samso-eum(Shensuyin) and Eunkyo-san(Yinqiao-san) caused lethal damage in the normal cells and also showed strong toxicity in the carcinoma cells.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 2000년도 제15차 학술회의논문집
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• pp.412-412
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• 2000

This paper presents a task teaching method for off-line programming of a multi-robot system. Teaching commands were developed in order to simplify a complex teaching process, to shorten the setup time for new working environment and to have flexibility for changes in working environment. Four teaching commands can be used to automatically generate trajectories of an end-effector of the robot in electronics assembly line. The robots used in the work cell are a four-axis SCARA robot and six-axis articulated robot. Each robot is controlled in a independent way while objects, working environment and robots are modeled in corresponding modules, respectively. The off- line programming system developed uses OpenGL for a smooth graphic effect in Window s where three dimensional CAD data can be leaded for graphical modeling.

• 한국산업융합학회 논문집
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• 제13권2호
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• pp.107-112
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• 2010

The automation and Cell-Line of manufacturing process are going to be new trend in the industry spot. But workers bodily burden by manual labour is still doing repeatedly at many processes. It is appearing to workers bodily burden (Shoulder, waist, hand, wrist, leg) with repeating works at assembly line which is from the static working space. The analysis with 6 Sigma Tool at specific standard assembly line improve the point at issue for unsuitable items and analyzed objects. Physical pain of worker is solved by the improvement action for the factor of 7 items with the result of analysis. It was known to be improved by solving of workers burden related to the change of 6 Sigma level from 2.16 to 4.1 at assembly line.