Browse > Article
http://dx.doi.org/10.15230/SCSK.2012.38.4.305

The New in vitro Oral Irritation Test Method for Toothpaste using YD-38 Oral Mucosal Cell Line  

Nam, Gi Baeg (R&D Center, Amorepacific Coporation)
Cho, Sun-A (R&D Center, Amorepacific Coporation)
Cho, Jun-Cheol (R&D Center, Amorepacific Coporation)
Kim, Chanho (R&D Center, Amorepacific Coporation)
Kim, Yoo-Jin (R&D Center, Amorepacific Coporation)
Lee, John Hwan (R&D Center, Amorepacific Coporation)
Shin, Kyeho (R&D Center, Amorepacific Coporation)
Publication Information
Journal of the Society of Cosmetic Scientists of Korea / v.38, no.4, 2012 , pp. 305-310 More about this Journal
Abstract
Through our entire life, oral care products such as toothpaste are used. Thus the safety of oral care products used every day to our mouth is very important. As the previous study in animal tests or clinical trials, surfactant in toothpaste may cause the oral irritation. However, EU cosmetics legislation prohibits animal testing of cosmetics and its ingredient for animal welfare. Therefore the development of alternative in vitro test has been actively performed to replace or reduce using the animal in many areas. However, the way to evaluate oral mucosal toxicity has been done using animal models or clinical trials from now on. Even more, the experiment with human oral 3D tissue or human oral cell line is used recently. The aim of this study is the development of oral mucosal irritation method without using animal for the safety of the oral care product. We developed in vitro test method for oral irritation by using human oral cell line (YD-38 cell) acceptable to toothpaste which contains insoluble material. By the results of this assay, we could discriminate toothpaste with or without irritating substance as same manner in animal studies reported previously. In addition, we confirmed that toothpaste for babies and children toothpaste irritated oral musoca lower than the general adult toothpaste. The present study suggest that this new in vitro method by using human oral cell line (YD-38 cell) could be used for evaluation of oral irritation without using animal.
Keywords
oral irritation; YD-38; toothpaste; alternative;
Citations & Related Records
연도 인용수 순위
  • Reference
1 I. Rantanen, K. Jutila, I. Nicander, J. Tenovuo, and E. Soderling, The effects of two sodium lauryl sulphate- containing toothpastes with and without betaine on human oral mucosa in vivo, Swedish Denal Journal, 27, 31 (2003).
2 E. E. Ahlfors, J. E. Dahl, and T. Lyberg, The development of T cell-dominated inflammatory responses induced by sodium lauryl sulphate in mouse oral mucosa, Archives of oral biology, 57(6), 796 (2012).   DOI   ScienceOn
3 E. Ahlfors and C. Czerkinsky, Contact sensitivity in the murine oral mucosa. I. An experimental model of delayed-type hypersensitivity reactions at mucosal surfaces, Clin Exp Immunol., 86(3), 449 (1991).   DOI   ScienceOn
4 P. Bourrinet, JP. Conduzorgues, H. Dutertre, J. Macabies, P. Masson, J. Maurin, and O. Mercier, Interlaboratory study for the assessment of potential irritative properties of hygiene products on the hamster cheek pouch, Lab Anim. Sci., 45(1), 36 (1995).
5 R. J. Veys, J. H. Baert, and J. A. De Boever, Histological changes in the hamster cheek pounch epithelium induced by topical application of sodium laul sulphate, Int. J. Path., 75, 203 (1994).
6 M. Klausner, S. Ayehunie, B. A. Breyfogle, P. W. Wertz, L. Bacca, and J. Kubilus, Organotypic human oral tissue models for toxicological studies, Toxicology in Vitro, 21, 938 (2007).   DOI   ScienceOn
7 T. Tsutsui, Y. Tanaka, A. Ushimura, T. Ide, M. Matsumura, and J. C. Barrett, In vitro cytotoxicity of diverse preparations used in dental practice to human gingival keratinocytes, Toxicology in Vitro, 11(4), 393 (1997).   DOI   ScienceOn
8 K. Moharamzadeh, H. Colley, C. Murdoch, V. Hearnden, W. L. Chai, I. M. Brook, M. H. Thornhill, and S. MacNeil, Tissue-engineered Oral Mucosa, Journal of Dental Research, 86, 115 (2007).   DOI   ScienceOn
9 J. P. Babich and H. Babich, Sodium lauryl sulfate and triclosan : In vitro cytotoxicity studies with gingival cells, Toxicology Letters, 91(3), 189 (1997).   DOI   ScienceOn