The Journal of Internal Korean Medicine (대한한방내과학회지)

The Society of Internal Korean Medicine (대한한방내과학회)
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Effects of Several Medicine Herb Prescriptions on Lung Carcinoma Cells

수종의 한약처방이 폐암세포에 미치는 영향

  • Kang, Pil-Gu (Dept. of Internal Medicine, College of Oriental Medicine, Dong Eui University) ;
  • Park, Dong-Il (Dept. of Internal Medicine, College of Oriental Medicine, Dong Eui University) ;
  • Choi, Won-Choul (Dept. of Biology, College of Natural Sciences, Pusan National University) ;
  • Jeon, Jong-Choul (Dept. of Internal Medicine, College of Oriental Medicine, Dong Eui University)
  • 강필구 (동의대학교 한의과대학 폐계내과학교실) ;
  • 박동일 (동의대학교 한의과대학 폐계내과학교실) ;
  • 최원철 (부산대학교 생물학과) ;
  • 전종철 (동의대학교 한의과대학 폐계내과학교실)
  • Published : 2000.12.30
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Abstract

Objective : In this study, the medicine herbs above were directly treated to cultured normal lung cells and lung carcinoma cells, and the effects were investigated to develope new cancer treatments with increased anti-cancer efficiency as well as decreased side-effects and to suggest more useful clinical therapies. Materials and Methods : In the experiment, WI-26 VA lung normal cell line and A-427 lung carcinoma cell line were cultured. To observe the morphological change of the treated cells, were subjected to Giemsa staining and observed under Reflected Fluorescence microscope. To examine whether cell death occurred, cells observed under Reflected Fluorescence microscope, To investigate the degree of cell death in the nucleus, cells were screened by Laser cytometry ACAS 570. Results : Samgibopye-tang(Shenqibufei-tang) stimulated not only the growth of the normal cells but also that of the carcinoma cells, Wikyeung-tang(Weijing-tang) induced morphological change such as cytoplasmic constriction in the normal cells and the carcinoma cells, but it did not show any strong inhibitory effect on the cell growth. Samso-eum(Shensu-yin) caused severe cell damage in both cell lines, Eunkyo San(Yinqiao-san) significantly damaged the nuclei and caused weak cytoplasmic constriction in both cell lines, Normal cells treated with Gilgyeung-tang(Jingeng-tang) did not show any significant morphological change while some Gilgyeung-tang(Jingeng-tang) treated carcinoma cells were observed to have a normal cell-like shape, interestingly, conclusions : As the results above, Samgibopye-tang(Shenqibufei-tang), Wikyeung-tang(Weijing-tang), and Gilgyeung-tang(Jingeng-tang) helped the growth of both cell lines, and especially Samgibopye-tang(Shenqibufei-tang) showed the best effect, However, Samso-eum(Shensuyin) and Eunkyo-san(Yinqiao-san) caused lethal damage in the normal cells and also showed strong toxicity in the carcinoma cells.

Keywords

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