• 한국염색가공학회:학술대회논문집
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• 한국염색가공학회 2008년도 제38차 학술발표대회
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• pp.25-27
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• 2008

The optimum extraction conditions of green tea used for a bath were investigated for later application to textile. The extraction was more effective in water and methanol than in ethanol. The optimum extraction temperature was determined as 80$^{\circ}C$ in water and 60$^{\circ}C$ in methanol. The extracts were more stable in acid and neutral conditions than in alkaline region. The UV satbility was better than generally expected. From the analysis of contents of active ingredients, the about 20% of effective catechin was appeared to be contained in the extract solid. The contents of polyphenol is 0.016g in the 0.1g extracts.

• 생약학회지
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• 제40권1호
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• pp.13-17
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• 2009

It was previously reported that yeast elicitor transiently increased oleanolic acid and ursolic acid in Scutellaria baicalensis suspension cultures and also doubled phospholipase $A_2$ ($PLA_2$) activity. Thus, $PLA_2$ was purified from the soluble fractions of S. baicalensis suspension cultures and the characters of the purified $PLA_2$ were identified. The $PLA_2$ was purified about 160 times compared with the starting soluble-protein extract from S. baicalensis suspension culture cells. The purified protein showed a molecular mass of about 43 kDa by SDS-PAGE. The purified plant $PLA_2$ had a neutral pH optimum (pH 7.0) and required $Ca^{2+}$ for activity. The $PLA_2$ activity was inhibited by mammalian $PLA_2$ inhibitors such as 5,8,11,14-eicosatetraynoic acid(ETYA) and arachidonyl trifluoromethyl ketone ($AACOCF_3$).

• 한국식물병리학회지
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• 제14권6호
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• pp.598-602
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• 1998

Twenty two rhizobacteria were isolated from the roots and rhizosphere of radish, carnation, potato and tomato. There isolates produced a fluorescent pigment in King's B medium and identified as Pseudomonas spp. These isolates colonized roots and rhizosphere of the host plants. In the study of cultural characteristics of the bacteria, the pH of the culture broth was changed from neutral (7.0) to alkali (8.8∼9.41) and the numbers of cells were increased from 106 to 108 after 40 hr of incubation in basal standard succinate medium. The salicylic acid production identified by pink color reaction were observed in 7 bacteria. Out of these 7 salicylic acid producing bacteria, only 2 strains of bacteria such as Pseudomonas fluorescens RS006, and Pseudomonas sp. EN401 were confirmed as salicylic acid producers by optical density measurement. Therefore, for screening of salicylic acid producing bacteria from the roots and rhizosphere, color reaction of the culture medium should be done in the first step, and then optical density measurement of culture extract should be made for the confirmation of salicylic acid production.

• Journal of Pharmaceutical Investigation
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• 제39권5호
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• pp.333-337
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• 2009

We aimed to optimize the formulation of porcine placental extract (PPE)-loaded liposomes for intramuscular administration and to investigate the effect of surface charges on the muscular retention in mice. PPE-loaded liposomes were formulated to have neutral, anionic, or cationic surface charges. The in vitro release profiles were studied by spectrofluorometry. In vivo distribution patterns at mice were studied using molecular imaging technology. Among the three types of liposomes, 1,2-dioleoyl-3-trimethylammonium-propane and 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-based cationic liposomes showed the most prolonged in vitro release profile. Consistent with the in vitro results, the in vivo distribution study revealed that the cationic liposomes were retained at the site of administration for the longest period. Our results suggest the potential of cationic PPE-loaded liposomes for sustained release of the components after intramuscular administration.

• Biotechnology and Bioprocess Engineering:BBE
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• 제7권2호
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• pp.100-104
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• 2002

Serratia marcescens biovar A2/A6, isolated from an Indonesian freshwater source, was identified based on extensive morphological, biochemical and genetic characterization. Formation of pigment was found to be strongly influenced by environmental conditions. Placket-Burman design was used to analyze the effect of carbon and nitrogen sources. Based on results of physiological and biochemical studies, the optimum conditions for growth and pigment formation were incubation 30$^{\circ}C$ in a neutral to slightly alkaline medium containing lactic acid and beef extract.

• 한국가정과학회지
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• 제5권2호
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• pp.119-127
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• 2002

Dyeing properties of the colorants extracted from acorn on wool fabric were investigated. Effects of dyeing temperature, dyeing time, repeating, and pH on dye uptake and colorfastness were explored. Acorn colorants showed very good affinity to wool fiber and produced bright to dark brown color ranged 4.81-8.97YR, 4.46-7.47/1.79-3.35. ,Higher temperature increased dye adsorption, so did longer time and more repeating. Washfastness was fairly good. The Dyed wool fabric had deeper color after irradiation to light. pH had an effect on the development of color, also. Dye uptake and washfastness were higher in acidic and alkaline pH than in neutral pH solution.

• 한국식품영양과학회지
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• 제27권2호
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• pp.333-337
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• 1998

This study was carried out to get infomation on the nitric oxide production ability and its formation mechanisms of polysaccharides extracted from Ganoderma lucidum(PSG) by using murine macrophage cell line. The cultured mycelial cells of Ganoderma lucidum were extracted by alkali, and than neutralized by acid. The extract were passed through the column of DEAE cellulose for more purification. The neutral fraction was concentrated and precipitated with 95% ethanol. The precipitate was lyophilized and PSG was obtained. The immunomodulating effects of PSG on macrophage were performed by using murine macrophage cell line ATCC TIB 71 cells with PSG 0.5mg. PSG alone could not induce the production of nitrite, but it had a significant potential effect on nitrite secretion when the cells were primed and triggered with BCG and Interferon(IFN)-${\gamma}$. Also it was prominent by using calcium channel blocker(verapamil) and adenylate cyclase activator(forskolin).

• 한국연초학회지
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• 제10권2호
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• pp.99-107
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• 1988

Pot experiment was conducted to find out the effect of lime application on yield and chemical composition of burley tobacco in 1986, Lime increased exchangeable calcium and pH of soil, but decreased Al, Fe and Mn concentrations. Yield was increased by lime application, however lime could not be caused to yield increasing in the soil with high calcium contents. Cored leaves of limed Plot contained higher Mg. K, total nitrogen, NO3-nitrogen, water soluble and insoluble ash, alkalinity number of water soluble and insoluble ash, citric and malic acid, but lower Fe, Mn, protein-nitrogen, NH3-nitrogen, nicotine petroleum ether extract, palmitic and linolenic acid concentrations than those of unlined plot. The linoleic acid and volatile neutral constituents of cured leaves were not affected by liming. Lime increased yield, however it did not affect leaf duality in respect to chemical characteristics, suggesting that liming should be necessary for tobacco cultivation.

• 한국식품영양과학회지
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• 제24권1호
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• pp.147-153
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• 1995

The cultured mycelial cells of Ganoderma lucidum was extracted by alkali, and then neutralized by acid. The extract was passed through the column of DEAE cellulose for more purification. The neutral fraction was concentrated and precipitated with 95% ethanol. The precipitate was lyophilized and then PSG(polysaccharides) was obtained. PSG was composed of 82.2% polysaccharide, 0.7% protein and 17.1% uronic acid. Sugar conjugates of its hydrolysates were produced using with fluorescent compound(7-amino-1,3-naphthalene disulfonic aicd : 7-AGA), and then fluorescent labeled sugar conjugates were separated by reverse phase high perfomance liquid chromatography. Hydrolysates of PSG were composed of sixteen amino acids and 95.7% glucose, 2.7% xylose, 1.6% fucose and tract amount of galactose and mannose. The immunomodulating effects of PSG on macrophage were perfomed using murine macrophage cell line ATCC TIB 71 cells. PSG augumented the phagocytic activity of TIB 71 cells against fluorescent latex beads.

• 한국식품영양학회지
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• 제30권2호
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• pp.388-394
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• 2017

본 연구에서는 생물전환 발효고려엉겅퀴 주정추출물을 온도별(4, 25 및 $50^{\circ}C$), pH별(4.0, 7.0, 및 10.0)로 21일간 저장하면서 7일 간격으로 pectolinarin과 pectolinarigenin 함량, 총 페놀 함량, DPPH 라디칼 소거능 및 FRAP를 측정하여 안정성을 평가하였다. 발효고려엉겅퀴 추출물인 pectolinarin과 pectolinarigenin 함량은 저장기간이 증가함에 따라 감소하는 경향을 보였으며, 온도에 의한 영향보다 pH에 의한 영향이 큰 것으로 나타났다. pH 10.0에서 pectolinarin의 함량 변화는 초기 $2.13{\pm}0.05mg/g$에서 $1.60{\pm}0.01mg/g$으로, pectolinarigenin 함량 변화는 $24.96{\pm}0.07mg/g$에서 $0.45{\pm}0.02mg/g$으로 크게 감소하였다. 총 페놀 함량도 저장기간이 따라 같이 감소하는 경향을 보였으며, $50^{\circ}C$와 pH 10.0에서 큰 감소치를 보였다. 항산화 안정성은 온도별 저장조건에서는 DPPH 라디칼 소거능은 큰 차이를 보이지 않았고, pH 10.0에서는 크게 감소하는 경향을 보였으나, FRAP은 온도별, pH별 저장조건에 따른 큰 변화를 보이지 않았다. 이상의 저장 온도별, pH별 결과를 종합해 볼 때, 생물전환 발효고려엉겅퀴 주정 추출물은 저온($4^{\circ}C$)에서 산성(pH 4.0)과 중성(pH 7.0) 상태에서 안정하였다.