Browse > Article

Purification of Phospholipase $A_2$ from Scutellaria baicalensis Suspension Cells  

Ma, Choong-Je (Department of Biomaterials Engineering, School of Bioscience and Biotechnology, Kangwon National University)
Kim, Dae-Kyung (College of Pharmacy, Chungang University)
Publication Information
Korean Journal of Pharmacognosy / v.40, no.1, 2009 , pp. 13-17 More about this Journal
Abstract
It was previously reported that yeast elicitor transiently increased oleanolic acid and ursolic acid in Scutellaria baicalensis suspension cultures and also doubled phospholipase $A_2$ ($PLA_2$) activity. Thus, $PLA_2$ was purified from the soluble fractions of S. baicalensis suspension cultures and the characters of the purified $PLA_2$ were identified. The $PLA_2$ was purified about 160 times compared with the starting soluble-protein extract from S. baicalensis suspension culture cells. The purified protein showed a molecular mass of about 43 kDa by SDS-PAGE. The purified plant $PLA_2$ had a neutral pH optimum (pH 7.0) and required $Ca^{2+}$ for activity. The $PLA_2$ activity was inhibited by mammalian $PLA_2$ inhibitors such as 5,8,11,14-eicosatetraynoic acid(ETYA) and arachidonyl trifluoromethyl ketone ($AACOCF_3$).
Keywords
arachidonyl trifluoromethyl ketone; 5,8,11,14-eicosatetraynoic acid; phospholipase $A_2$; Scutellaria baicalensis;
Citations & Related Records

Times Cited By SCOPUS : 0
연도 인용수 순위
  • Reference
1 Ishiguro, S., Kawai-Oda, A., Ueda, J., Nishida, I. and Okada, K. (2001) The defective in anther dehiscence1 gene encodes a novel phospholipase A1 catalyzing the initial step of jasmonic acid biosynthesis, which synchronizes pollen maturation, anther dehiscence, and flower opening in arabidopsis. Plant Cell 13: 2191-2209   DOI   ScienceOn
2 Ma, C. J. (2008) Cellulase elicitor induced accumulation of capsidiol in Capsicum annumm L. suspension cultures. Biotech. Lett. 30: 961-965   DOI   PUBMED   ScienceOn
3 Dole V.P. and Meimertz H. (1960) Microdetermination of long-chain fatty acids in plasma and tissues. J. Biol. Chem. 235: 2595-2599   PUBMED
4 Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685   DOI   PUBMED   ScienceOn
5 Rordorf, G., Uemura, Y. and Bonventre, J. V. (1991) Characterization of phospholipase $A_{2}$ ($PLA_{2}$) activity in gerbil brain: enhanced activities of cytosolic, mitochondrial, and microsomal forms after ischemia and reperfusion. J. Neurosci. 11: 1829-1836   PUBMED
6 Munnik, T., Irvine, R. F. and Musgrave, A. (1998) Phospholipid signaling in plants. Biochim. Biophys. Acta 1389: 222-272   DOI   PUBMED   ScienceOn
7 Jung, K. M. and Kim, D. K. (2000) Purification and characterization of a membrane-associated 48-kilodalton phospholipase $A_{2}$ in leaves of broad bean. Plant Physiol. 123: 1057-1067   DOI   ScienceOn
8 Bergey, D. R., Howe, G. A. and Ryan, C. A. (1996) Polypeptide signaling for plant defensive genes exhibits analogies to defense signaling in animals. Proc. Natl. Acad. Sci. USA 93: 12053-12058   DOI   ScienceOn
9 Creelman, R. A. and Mullet, J. E. (1997) Biosynthesis and action of jasmonates in plants. Annu. Rev. Plant Physiol. Plant Mol. Biol. 48: 355-381   DOI   ScienceOn
10 Yoon, H. J., Kim, H. K., Ma, C. J. and Huh, H. (2000) Induced accumulation of triterpenoids in Scutellaria baicalensis suspension cultures using a yeast elicitor. Biotech. Lett. 22: 1071-1075   DOI   ScienceOn
11 Dennis, E. A., Rhee, S. G., Billah, M. M. and Hannun, Y. A. (1991) Role of phospholipases in generating lipid second messengers in signal transduction. FASEB J. 5: 2068-2077   DOI   PUBMED   ScienceOn
12 Street, I. P., Lin, H. K., Laliberte, F., Ghomashchi, F., Wang, Z., Perrier, H., Tremblay, N. M., Huang, Z., Weech, P. K. and Gelb, M. H. (1993) Slow- and tight-binding inhibitors of the 85-kDa human phospholipase $A_{2}$. Biochemistry 32: 5935-5940   DOI   ScienceOn
13 Divchev, D. and Schieffer, B. (2008) The secretory phospholipase $A_{2}$group IIA: a missing link between inflammation, activated rennin-angiotensin system and atherogenesis? Vascul. Health Risk Manag. 4: 597-604   DOI
14 Ackermann, E. J., Conde-Frieboes, K. and Dennis, E. A. (1995) Inhibition of macrophage$Ca^{2+}$+-dependent phospholipase $A_{2}$ by bromoenol lactone and trifluoromethyl ketones. J. Biol. Chem. 270: 445-450   DOI   ScienceOn
15 Kudo, I., Murakami, M., Hara, S. and Inoue, K. (1993) Mammalian non-pancreatic phospholipases $A_{2}$. Biochim. Biophys. Acta 1170: 217-231   DOI   PUBMED   ScienceOn
16 Scherer, G. F. E. and Arnold, B. (1997) Inhibitors of animal phospholipase A2 enzymes are selective inhibitors of auxin dependent growth: implications for auxin-induced signal transduction. Planta 202: 462-469   DOI   ScienceOn