Journal of Pharmaceutical Investigation

The Korean Society of Pharmaceutical Sciences and Technology (한국약제학회)
권호 표지
DOI QR코드

DOI QR Code

Effects of Surface Charges on the Retention of Placenta-loaded Liposome Formulations Administered by Intramuscular Route

  • Noh, Sang-Myoung (School of Pharmacy, Seoul National University) ;
  • Park, Da-Eui (School of Life Science and Biotechnology, Korea University) ;
  • Kim, Young-Bong (Department of Animal Biotechnology, Konkuk University) ;
  • Oh, Yu-Kyoung (School of Pharmacy, Seoul National University)
  • Published : 2009.10.20
Download PDF
⟨ Previous Next ⟩

Abstract

We aimed to optimize the formulation of porcine placental extract (PPE)-loaded liposomes for intramuscular administration and to investigate the effect of surface charges on the muscular retention in mice. PPE-loaded liposomes were formulated to have neutral, anionic, or cationic surface charges. The in vitro release profiles were studied by spectrofluorometry. In vivo distribution patterns at mice were studied using molecular imaging technology. Among the three types of liposomes, 1,2-dioleoyl-3-trimethylammonium-propane and 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-based cationic liposomes showed the most prolonged in vitro release profile. Consistent with the in vitro results, the in vivo distribution study revealed that the cationic liposomes were retained at the site of administration for the longest period. Our results suggest the potential of cationic PPE-loaded liposomes for sustained release of the components after intramuscular administration.

Keywords

References

  1. S. Togashi, N. Takahashi, M. Iwama, S. Watanabe, K. Tamagawa and T. Fukui, Antioxidative collagen-derived peptides in human-placenta extract, Placenta, 23, 497-502 (2002). https://doi.org/10.1053/plac.2002.0833
  2. S. Watanabe, S. Togashi, N. Takahashi and T. Fukui, L-tryptophan as an antioxidant in human placenta extract, J. Nut. Sci. Vit., 48, 36-39 (2002). https://doi.org/10.3177/jnsv.48.36
  3. G. Tonello, M. Daglio, N. Zaccarelli, E. Sottofattori, M. Mazzei and A. Balbi, Characterization and quantitation of the active polynucleotide fraction (PDRN) from human placenta, a tissue repair stimulating agent, J. Pharm. Biomed. Anal., 14: 1555-1560 (1996). https://doi.org/10.1016/0731-7085(96)01788-8
  4. H.R. Cho, J.H. Ryou, J.W. Lee and M.H. Lee, The effects of placental extract on fibroblast proliferation, J. Cosmet. Sci., 59, 195-202 (2008).
  5. H.S. Kim, J.H. Cho, H.W. Park, H. Yoon, M.S. Kim and S.C. Kim, Endotoxin-neutralizing antimicrobial proteins of the human placenta, J. Immunol., 168, 2356-2354 (2002). https://doi.org/10.4049/jimmunol.168.5.2356
  6. D. Chakraborty, J.M. Basu, P. Sen, S. Sundar and S. Roy, Human placental extract offers protection against experimental visceral leishmaniasis: a pilot study for a phase-I clinical trial, Ann. Trop. Med. Parasitol., 102, 21-38 (2008). https://doi.org/10.1179/136485908X252133
  7. V. Kaushal, K. Verma, S. Manocha, H.S. Hooda and B.P. Das, Clinical evaluation of human placental extract (placentrex) in radiation-induced oral mucositis, Int. J. Tissue React., 23, 105- 110 (2001)
  8. M.V. Langston, M.P. Ramprasad, T.T. Kararli, G.R. Galluppi and N.V. Katre, Modulation of the sustained delivery of myelopoietin (Leridistim) encapsulated in multivesicular liposomes (DepoFoam), J. Control. Rel., 89, 87-99 (2003). https://doi.org/10.1016/S0168-3659(03)00073-7
  9. H. Zhong, Y. Deng, X. Wang and B. Yang, Multivesicular liposome formulation for the sustained delivery of breviscapine, Int. J. Pharm., 301, 15-24 (2005). https://doi.org/10.1016/j.ijpharm.2005.04.001
  10. S. Mantripragada, A lipid based depot (DepoFoam technology) for sustained release drug delivery, Prog. Lipid Res., 41, 392- 406 (2002). https://doi.org/10.1016/S0163-7827(02)00004-8
  11. M. Meng, Y. Liu, Y.-B. Wang, J.-C. Wang, H. Zhang, X.-Q. Wang, X. Zhang, W.-L. Lu and Q. Zhang, Increase of the pharmacological and pharmacokinetic efficacy of negatively charged polypeptide recombinant hirudin in rats via parenteral route by association with cationic liposomes, J. Control. Rel. 128, 113-119 (2008). https://doi.org/10.1016/j.jconrel.2008.03.001
  12. Z. Pavelic, N. Skalko-Basnet and I. Jalsenjak, Characterisation and in vitro evaluation of bioadhesive liposome gels for local therapy of vaginitis, Int. J. Pharm., 301, 140-148 (2005). https://doi.org/10.1016/j.ijpharm.2005.05.022