The Influence of Environmental Conditions on the Production of Pigment by Serratia marcescens

  • Hardjito, Linawati (Center for Coastal and Marine Resources Studies, Bogor Agricultural University and Inter University Research Center on Biotechnology, Bandung Institute of Technology, Indonesia) ;
  • Huq, Anwar (Center of Marine Biotechnology, University of Maryland Biotechnology Institute, Baltimore, Maryland, USA and Department of Cell and Molecular Biology, University of Maryland, College Park) ;
  • Colwell, Rita R. (Center of Marine Biotechnology, University of Maryland Biotechnology Institute, Baltimore, Maryland, USA and Department of Cell and Molecular Biology, University of Maryland, College Park)
  • Published : 2002.03.01

Abstract

Serratia marcescens biovar A2/A6, isolated from an Indonesian freshwater source, was identified based on extensive morphological, biochemical and genetic characterization. Formation of pigment was found to be strongly influenced by environmental conditions. Placket-Burman design was used to analyze the effect of carbon and nitrogen sources. Based on results of physiological and biochemical studies, the optimum conditions for growth and pigment formation were incubation 30$^{\circ}C$ in a neutral to slightly alkaline medium containing lactic acid and beef extract.

Keywords

References

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