• 생명과학회지
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• 제12권3호
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• pp.369-373
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• 2002

Bacillus thrungensis를 이용한 미생물 살충제를 생산할 목적으로 두부폐수를 이용하여 내생포자 생산능을 검토하였다. 실험에 사용한 두부폐수원액의 성상은 pH 9.8, COD 276mg/$\ell$, 전질소량 71.1mg/$\ell$, 전인량 5.52mg/$\ell$ 이며 Bacillus thrungensis균을 대량배양하기 위한 기질로서의 이용 가능성을 확인한 결과, 두부폐수원액에서 가장 높은 포자형성능이 관찰 되었으며, 두부폐수원액의 첨가량이 작을수록 포자형성은 감소하는 경향을 나타내었다. 최대 포자형 성능은 1% $K_2$HPO4i 첨가때 가장 높은 포자형 성능을 나타내었다. 두부 공업폐수를 이용한 Bacillus thrungensis의 최적배지 조건에서 시간별 포자형 성능을 확인한 결과, 동일 시간당 1.5배 높은 포자형 성능이 확인되었다. 두부폐수는 Bacillus thrungensis를 생산하기 위한 적당한 배양기질로서 이용될 수 있을 것으로 생각되어 진다.

• 농업생명과학연구
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• 제44권3호
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• pp.23-30
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• 2010

본 실험은 Dendrobium nobile 계통의 교잡종인 'Hamana Lake Dream' ${\times}$ 'No. 55' 유묘의 기내삽목 시 증식 및 생육에 알맞은 배지와 배양조건을 구명하고자 실시하였다. Hyponex배지보다 MS배지에서 생장이 잘되었는데 MS배지에 활성탄을 0.1과 1.0g/L 수준에서 첨가했을 때 줄기수와 엽장, 근수, 생체중이 양호 하였으나 2.0g/L이상에서는 감소하였으나 통계적 유의성은 없었다. MS배지에 agar, sucrose, peptone, gelite 를 농도별로 첨가한 실험에서는 agar 5g/L 첨가 시 줄기 길이와 엽폭, 생체중의 값이 높았고, 절간장과 근수는 sucrose 40g/L을 첨가했을 때 효과적이었다. 또한 줄기수와 엽수는 peptone 1g/L에서 효과적이었으나 gelite는 모든 농도에서 대조구에 비해 저조하였다. 기내삽목 시 배양온도는 $28^{\circ}C$가 알맞았고 동일한 온도조건에서는 MS배지가 Hyponex배지보다 더 효과적이었다. 또한 sucrose 농도별 실험에서는 $28^{\circ}C$에서 40 g/L를 첨가하는 것이 좋았다.

• Journal of Microbiology
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• 제39권1호
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• pp.17-23
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• 2001

Various pretreatment procedures and selective media were applied to assess the optimal conditions for the isolation of rare actinomycetes from soil. Pretreatment of wet-heating for 15 min at 70$^{\circ}C$ and phenol treatment of soil suspension were the most effective methods for the isolation of these microorganisms. Hair hydrolysate vitamin agar (HHVA) was the most suitable medium for the recovery of rare actinomycetes. Thirty-five rare actinomycete strains were chosen using selective isolation approaches, then morphological and chemical properties of the isolates were determined. The isolates belonged to one of the following genus, Micromonospora, Microbispora, Actinoplanes and Streptosporangium.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2001년도 추계학술발표대회
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• pp.841-844
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• 2001

Free radicals cause many diseases and aging. In our body coexist useful and harmful bacteria. Some useful intestinal bacteria such as L. plantarum, B. adolescentis, B .bifidum, B. infantis, C. butyricum were presented positive growth in culture medium bearing natural products. L. plantarum was more effectively grown by mixed natural products of CORNI FRUCTUS+THEAE FOLIUM+COPTIDIS RHIZOMA+PAEONIA JAPONICA, B. adolescentis was BEIAMCANDA CHINENSIS+ANGELICAE GIGANTIS RADIX+EPIMEDII HERBA+PAEONIA JAPONICA, B. bifidum was NELUMBO NUCLFERA GAERTNER+THEAE FOLIUM+BEIAMCANDA CHINENSIS+CORNI FRUCTUS, B. infantis was THEAE FOLIUM+CHELIDONIUM MAJUS L.+ASTRAGALI RADIX+EPIMEDII HERBA, C. butyricum was EPIMEDII HERBA+PUERARIAE RADIX+NELUMBO NUCLFERA GAERTNER+PAEONIA JAPONICA,etc. These natural products will support an important role in bio-industry.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2003년도 생물공학의 동향(XII)
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• pp.249-252
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• 2003

Maximal production of heteropolysaccharide-7(PS-7) with a batch culture for 48 hr was $10.0\;g/{\ell}$ and its conversion rate from 2% (w/v) glucose to PS-7 was 50%. After substitution of media, production of PS-7 continued and reached its maximal production. The highest productivity occurred when the fresh medium, which contained all ingredients, for the production of PS-7 was substituted. Higher production of PS-7 was maintained at a dilution rate of 0.0125, which was established as the optimal dilution rate for the production of PS-7 by B. indica HS-2001.

• Journal of Microbiology and Biotechnology
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• 제11권1호
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• pp.56-60
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• 2001

A kerosene fungus of Cladosporium resinae NK-1 was examined for its ability to degrade individual n-alkanes and aromatic hydrocarbons by gas chromatography-mass spectrometry, and its organic solvent-tolerance was investigated by making use of the water-organic solvent suspension culture method. It grew on a wide range of solvents of varying hydrophobicities and it was found to have tolerance to various kinds of toxic organic solvents (10%, v/v) such as n-alkanes, cyclohexane, xylene, styrene, and toluene. A hydrocarbon degradation experiment indicated that NK-1 had a greater n-alkane degrading ability compared to that of the other selected strains. C. resinae NK-1, which could utilize 8-16 carbon chain-length n-alkanes of medium chain-length as a carbon source, could not assimilate the shorter chain-length n-alkanes and aromatic hydrocarbons tested so far. The n-alkane degrading enzyme activity was found in the mycelial extract of the organism.

• 한국자원리싸이클링학회:학술대회논문집
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• 한국자원리싸이클링학회 2001년도 정기총회 특별강연 및 춘계학술연구발표회(2)
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• pp.121-125
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• 2001

For the probiotic feed production, aerobic liquid fermentation of pulverized food wastes was attempted with a yeast Kluyveromyces marxianus. After grinding finely, optimal fermentation conditions of the substrate was investigated by shaking culture. The most active growth of the yeast was shown at solid content of 10%. The proper addition of urea(0.5g/l), o-phosphate(0.4g/l), molasses(4g/l), and yeast extract (1g/1) increased cell growth rate and viable cell count. For optimizing, the nutrients were all added to substrate and fermentation was carried in 2 litre jar fermenter. For the stimulation of hydrolyzing enzyme excretion, mixed culture with Aspersillus oryzae was also conducted. In 12 hours of fermentation, viable cell count of the yeast Kluyveromyces marxianus amounted to the number of 1.4 $\times$10$^{10}$ /1 in the culture medium.

• 대한기계학회논문집B
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• 제22권12호
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• pp.1796-1804
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• 1998

A numerical investigation has been performed to discuss the radiation-affected steady-laminar natural convection induced by a hot inner cylinder under a large temperature difference in the annuli filled with a gray gas. To examine the effects of thermal radiation on thermo-fluid dynamic behaviors in the eccentric geometry, the generalized body-fitted coordinate system is introduced while the finite volume method (FVM) is used for solving the radiative transport equation. After validating the numerical results for the case without radiation, the detailed radiation effect has been discussed. Based on the results of this study, when there exists a large temperature difference between two cylinders, the existence of radiatively participating medium is found to incur a distinct difference in fluid dynamic as well as thermal behavior.

• 한국염색가공학회지
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• 제7권2호
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• pp.47-54
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• 1995

Safflower is natural red dye largely used for dyeing on cotton. It contains yellowish safflower yellow and reddish carthamin, whose constitution is known. Safflower yellow is water-soluble dye and carthamin is solved in alkaline condition. Carthamon obtained by adding acidic solution to a carthamin shows the original hue of safflower. In present study, the dyeing behavior of natural dye known as safflower are examined in an aqueous acidic medium by use of the different dyeing methods such as traditional, modern and purifide-powder method. The relationship between the dye-uptake of cotton fabric investigated by the three methods and the various dyeing conditions is discussed.

• 한국응용과학기술학회지
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• 제16권1호
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• pp.67-73
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• 1999

Chitosan and Algin are known as a natural polymers. Biodegradable films were prepared by solution blend method in the weight ratio of natural polymer(low, medium, high-Chitosan, Algin) for the purpose of useful bioimplants. The possibility of bioimplants, which were prepared from natural polymers as a skin substitute and food wrapping materials were evaluated by measuring biodegradability. This biodegradable films were inserted in the back of rats and their biodegradability was investigated by hematological change evaluation as a function of time to biotransformation. It was found that these values of biodegradable films give some good results with short period test.