Journal of agriculture & life science (농업생명과학연구)

Institute of Agriculture & Life Science, Gyeongsang National University (경상대학교 농업생명과학연구원)
권호 표지

Effects of Media and Temperatures on Micro Stem Cutting of Dendrobium nobile 'Hamana Lake Dream' × 'No. 55' in Vitro

Dendrobium nobile 'Hamana Lake Dream' × 'No.55'의 기내 줄기삽목에 미치는 배지의 종류와 온도의 영향

  • Yoon, Jin-Young (Dept. of Horticultural Science and Landscape Architecture, College of Natural Science, Seoul Women's University) ;
  • Nam, Yu-Kyeong (Dept. of Horticultural Science and Landscape Architecture, College of Natural Science, Seoul Women's University) ;
  • Lee, Jong-Suk (Dept. of Horticultural Science and Landscape Architecture, College of Natural Science, Seoul Women's University) ;
  • Kim, Hyun-Jin (Dept. of Horticultural Science and Landscape Architecture, College of Natural Science, Seoul Women's University)
  • 윤진영 (서울여자대학교 자연과학대학 원예조경학과) ;
  • 남유경 (서울여자대학교 자연과학대학 원예조경학과) ;
  • 이종석 (서울여자대학교 자연과학대학 원예조경학과) ;
  • 김현진 (서울여자대학교 자연과학대학 원예조경학과)
  • Received : 2010.04.06
  • Accepted : 2010.06.15
  • Published : 2010.06.30
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Abstract

The optimal growth condition of in vitro stem cutting of Dendrobium nobile 'Hamana Lake Dream' ${\times}$ 'No. 55' was investigated. Among various media and their concentrations, MS media had better effect on the growth of micro stem cutting than Hyponex media in all concentration levels except stem length. The activated charcoal concentration in MS media showed different effects on number of stem and root, leaf length, and fresh weight: the most effective in the range of 0.1 to 1.0 g/L and barely effective above 2.0 g/L. Addition of agar 5 g/L, sucrose at 40 g/L, and peptone at 1 g/L to MS media increased significantly stem length, leaf width, and fresh weight, internode length and number of roots, and the number of stem and leaves. On the other hand, addition of gelite with any concentration had no effect on the growth of micro stem cutting compare to that of control. The optimal temperature for growth of micro stem cutting was $28^{\circ}C$. Under the same temperature, MS medium was better than Hyponex medium for the growth of stem. In addition, sucrose at 40 g/L was the most effective on growth at $28^{\circ}C$.

본 실험은 Dendrobium nobile 계통의 교잡종인 'Hamana Lake Dream' ${\times}$ 'No. 55' 유묘의 기내삽목 시 증식 및 생육에 알맞은 배지와 배양조건을 구명하고자 실시하였다. Hyponex배지보다 MS배지에서 생장이 잘되었는데 MS배지에 활성탄을 0.1과 1.0g/L 수준에서 첨가했을 때 줄기수와 엽장, 근수, 생체중이 양호 하였으나 2.0g/L이상에서는 감소하였으나 통계적 유의성은 없었다. MS배지에 agar, sucrose, peptone, gelite 를 농도별로 첨가한 실험에서는 agar 5g/L 첨가 시 줄기 길이와 엽폭, 생체중의 값이 높았고, 절간장과 근수는 sucrose 40g/L을 첨가했을 때 효과적이었다. 또한 줄기수와 엽수는 peptone 1g/L에서 효과적이었으나 gelite는 모든 농도에서 대조구에 비해 저조하였다. 기내삽목 시 배양온도는 $28^{\circ}C$가 알맞았고 동일한 온도조건에서는 MS배지가 Hyponex배지보다 더 효과적이었다. 또한 sucrose 농도별 실험에서는 $28^{\circ}C$에서 40 g/L를 첨가하는 것이 좋았다.

Keywords

Acknowledgement

Supported by : 농림수산식품부

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