An Improved Selective Isolation of Rare Actinomycetes from Forest Soil

  • Seong, Chi-Nam (Department of Biology, College of Natural Sciences, Sunchon National University) ;
  • Park, Ji-Heok (Department of Biology, College of Natural Sciences, Sunchon National University) ;
  • Baik, Keun-Shik (Department of Biology, College of Natural Sciences, Sunchon National University)
  • 발행 : 2001.03.01

초록

Various pretreatment procedures and selective media were applied to assess the optimal conditions for the isolation of rare actinomycetes from soil. Pretreatment of wet-heating for 15 min at 70$^{\circ}C$ and phenol treatment of soil suspension were the most effective methods for the isolation of these microorganisms. Hair hydrolysate vitamin agar (HHVA) was the most suitable medium for the recovery of rare actinomycetes. Thirty-five rare actinomycete strains were chosen using selective isolation approaches, then morphological and chemical properties of the isolates were determined. The isolates belonged to one of the following genus, Micromonospora, Microbispora, Actinoplanes and Streptosporangium.

키워드

참고문헌

  1. K. J. Appl. Microbiol. Biotechnol. v.22 A new medium for the selective isolation of soil actinomycetes Cho S.H.;C.W. Hwang;H.K. Chung;C.S. Yang
  2. Analyst. v.101 Simplified method for the measurement of total non-starch polysaccharides by gas-chromatography of constituent sugars as alditol acetates Englyst H.N.;J.H. Cummings
  3. Bergey's manual of systematic bacteriology Suprageneric classification of actinomycetes Goodfellow M.;S.T. Williams(eds.);M.E. Sharpe(eds.);J.G. Holt(eds.)
  4. J. Ferment. Technol. v.65 Efficacy of artificial humic acid as a selective nutrient in HV agar used for the isolation of soil actinomycetes Hayakawa M.;H. Nonomura
  5. J. Ferment. Technol. v.65 Humic acid-vitamin agar, a new medium for the selective isolation of soil actinomycetes Hayakawa M.;H. Nonomura
  6. J. Ferment. Technol. v.72 New methods for the highly selective isolation of Micromonospora and Microbispora Hayakawa M.;T. Sadaka;T. Kajiura;H. Nonomura
  7. The Search for bioactive compounds from microorganisms Selection of microbial sources of bioactive comounds Iwai H.;Y. Takahashi;S. Oumra(ed.)
  8. Microbiol. Rev. v.55 Iso- and anteiso-fatty acids in bacteria; biosynthesis, function, and taxonomic significance Kaneda T.
  9. K. J. Appl. Microbiol. Biotechnol. v.23 Isolation of rare actinomycetes on various types of soil Kim C.J.;K.H. Lee;A. Shimazu;O.S. Kwon;D.J. Park
  10. K. J. Appl. Microbiol. Biotechnol. v.22 Selective isolation of actinomycetes by physical pretreatment of soil sample Kim C.J.;K.H. Lee;O.S. Kwon;A. Shimazu;I.D. Yoo
  11. J. Microbiol. v.34 Lipid analysis of streptomycetes isolated from volcanic soil Kim S.B.;M.Y. Kim;C.N. Seong;Y.C. Hah
  12. Ann. Rev. Microbiol. v.21 Biology of actinomycetes Lechevalier H.A.;M.P. Lechevalier
  13. J. Bacteriol. v.105 Lipid composition in the classification of nocardiae and mycobacteria Lechevalier M.P.;A.C. Horan;H.A. Lechevalier
  14. Actinomycete taxonomy, Society for Industrial Microbiology The chemotaxonomy of actinomycetes Lechevalier H.A.;M.P. Lechevalier
  15. Microbiological classification and identification Lipid composition in the classification and identification of acid-fast bacteria Minnikin D.E.;M. Goodfellow;M. Goodfellow(eds.);R.G. Board(eds.)
  16. J. Microbiol. Methods v.2 An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids Minnikin D.E.;A.G. O'Donnell;M. Goodfellow;G. Alderson;M. Athalye;A. Schaal;J.H. Parlett
  17. J. Chem. Technol. Biotechnol. v.32 Current strategies in the search for bioactive microbial metabolites Nisbet L.J.
  18. Actinomycetes in biotechnology Isolation and screening of actinomycetes Nolan R.D.;T. Cross;M. Goodfellow(eds.);S.T. Williams(eds.);M. Mordarski(eds.)
  19. Actinomycetol. v.3 Isolation, taxonomy and ecology of soil actinomycetes Nonomura H.
  20. Computer assisted bacterial systematics Numerical analysis of chemotaxanomic data O'Donnell A.G.;M. Goodfellow(eds.);;D. Jones(eds.);F.G. Priest(eds.)
  21. Actinomycetes in biotechnology Search and discovery of new antibiotics Okami Y.;K. Hotta;M. Goodfellow(eds.);S.T. Williams(eds.);M. Mordarski(eds.)
  22. J. Microorganism v.3 Rare actinomycetes -new breed of actinomycetes Okazaki T.
  23. D. Thesis Seong C.N.
  24. J. Microbiol. v.37 Mycolic acid-containing actinomycetes associated with activated sludge foam Seong C.N.;Y.S. Kim;K.S. Baik;S.D. Lee;Y.C. Hah;S.B. Kim;M. Goodfellow
  25. Int. J. Syst. Bacteriol. v.16 Methods for characterization of Streptomyces species Shirling E.B.;D. Gottlieb