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http://dx.doi.org/10.5352/JLS.2002.12.3.369

Production of Microbial Pesticides by Soybean Curd Waste-water in Bacillus thuringiensis subsp. kurstaki HD-1  

Ok, Min (Faculty of Natural Resource and Life Science, Dong-A University)
Kim, Dae-Jin (Faculty of Food and Nutrition, Dong-A University)
Lee, Young-Chun (Faculty of Natural Resource and Life Science, Dong-A University)
Choi, Yong-Lak (Faculty of Natural Resource and Life Science, Dong-A University)
Cho, Young-Su (Faculty of Natural Resource and Life Science, Dong-A University)
Publication Information
Journal of Life Science / v.12, no.3, 2002 , pp. 369-373 More about this Journal
Abstract
The waste-water from the industry for production of a soybean curd (the soybean curd waste-water) was investigated to use for the substrate to produce the endotoxin of Bacillus thuringiensis subsp. kurstaki HD-1 used as one of well known microbial pesticides. The pH of the soybean curd waste-water was 9.8 and its chemical oxygen demand (COD), total nitrogen (TN) and phosphate (TP) were 276.0, 71.1 and 5.5mg/$\ell$, respectively. The higher was the concentration of the soybean curd waste-water in the medium, the more endotoxin was produced. Maximal sporulation occurred at which concentration of $K_2$HPO$_4$in the medium supplied with the soybean curd waste-water was 1% (w/v). Production of the endotoxin with the optimized medium supplied with the soybean curd waste-water was 1.5 times higher than that without the soybean curd waste-water. The soybean curd waste-water was found to be suitable substrate for production of the endotoxin of Bacillus thuringiensis subsp. kurstaki HD-1.
Keywords
endotoxin; icrobial pesticide; soybean curd waste-water; Bacillus thuringiensis kurstaki HD-1;
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