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Effects of Media and Temperatures on Micro Stem Cutting of Dendrobium nobile 'Hamana Lake Dream' × 'No. 55' in Vitro  

Yoon, Jin-Young (Dept. of Horticultural Science and Landscape Architecture, College of Natural Science, Seoul Women's University)
Nam, Yu-Kyeong (Dept. of Horticultural Science and Landscape Architecture, College of Natural Science, Seoul Women's University)
Lee, Jong-Suk (Dept. of Horticultural Science and Landscape Architecture, College of Natural Science, Seoul Women's University)
Kim, Hyun-Jin (Dept. of Horticultural Science and Landscape Architecture, College of Natural Science, Seoul Women's University)
Publication Information
Journal of agriculture & life science / v.44, no.3, 2010 , pp. 23-30 More about this Journal
Abstract
The optimal growth condition of in vitro stem cutting of Dendrobium nobile 'Hamana Lake Dream' ${\times}$ 'No. 55' was investigated. Among various media and their concentrations, MS media had better effect on the growth of micro stem cutting than Hyponex media in all concentration levels except stem length. The activated charcoal concentration in MS media showed different effects on number of stem and root, leaf length, and fresh weight: the most effective in the range of 0.1 to 1.0 g/L and barely effective above 2.0 g/L. Addition of agar 5 g/L, sucrose at 40 g/L, and peptone at 1 g/L to MS media increased significantly stem length, leaf width, and fresh weight, internode length and number of roots, and the number of stem and leaves. On the other hand, addition of gelite with any concentration had no effect on the growth of micro stem cutting compare to that of control. The optimal temperature for growth of micro stem cutting was $28^{\circ}C$. Under the same temperature, MS medium was better than Hyponex medium for the growth of stem. In addition, sucrose at 40 g/L was the most effective on growth at $28^{\circ}C$.
Keywords
Activated charcoal; MS medium; Sucrose; Temperature;
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Times Cited By KSCI : 1  (Citation Analysis)
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